Core--Genetics

核心--遗传学

• 批准号: 6943670
• 负责人: Phillip Frazier CHANCE
• 金额: $ 10.21万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-07-01 至 2009-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6943670
• 关键词: biomedical equipment resource; biomedical facility; cell line; chromosome disorders; computer assisted sequence analysis; computer program /software; congenital brain disorder; cytogenetics; gene expression profiling; genetic mapping; genetic regulation; mental retardation; microarray technology; molecular genetics; polymerase chain reaction; tissue /cell culture; tissue resource /registry

DESCRIPTION: This core consists of three components: microarray, cell storage, and shared equipment. The microarray component will utilize the microarray facility at the Center for Ecogenetics and Environmental Health located adjacent to the main University of Washington campus. The facility will help the Center investigators to perform gene expression profiling studies using both cDNA and oligonucleotide microarrays. It provides experimental design consultation, RNA quality control, probe labeling, hybridization, and scanning services to the Center investigators. It also provides bioinformatics resources, including several software programs for data analyses. This component is well staffed. It consists of a coordinator, Dr. Philip Mirkes, Research Professor of Pediatrics, Microarray Laboratory Director Dr. Fred Farin, microarray design and analysis coordinator Dr. Theo Bammler, Microarray Research Scientist Sean Quigley, and Bioinformatics Consultant Dr. Richard Beyer. The cell storage facility consists of a liquid nitrogen tank with a capacity of 25,000 vials. It is for the Center investigators to store fibroblast and lymphoblast cell lines derived from patients and relatives with rare genetic diseases. It has a computerized log of all cell lines for the investigators to keep track of the location and usage of the cell lines. The shared equipment component contains several large pieces of equipment, including an ABI Prism 310 Genetic Analyzer for genotyping and sequencing, the Varian Helix dHPLC system for the detection and analysis of single base pair mutations, a new Mini-Med film processor. It also houses several pieces of basic laboratory equipment, such as ultra and high speed centrifuges, orbital shaker, drying oven, X-ray cross linker, gel dryer, vacuum concentrators, water distill, deionizer, spectrophotometer, a Bio-Rad Gel Doc 2000, and a Bio-Rad Gene Pulser II. The cell storage and shared equipment components are maintained and coordinated by Dr. Thomas Cotner, Research Associated Professor of Pediatrics. A total of 27 Center investigators and 37 projects are listed to use the various components of the core.

描述：该核心由三个部分组成：微阵列、细胞存储和共享 设备。微阵列组件将利用位于华盛顿大学主校区附近的生态遗传学和环境健康中心的微阵列设施。该设施将帮助中心研究人员使用 cDNA 和寡核苷酸微阵列进行基因表达谱研究。它为中心研究人员提供实验设计咨询、RNA质量控制、探针标记、杂交和扫描服务。它还提供生物信息学资源，包括多个用于数据分析的软件程序。该部分人员配备齐全。它由协调员 Philip Mirkes 博士、儿科研究教授、微阵列实验室主任 Fred Farin 博士、微阵列设计和分析协调员 Theo Bammler 博士、微阵列研究科学家 Sean Quigley 和生物信息学顾问 Richard Beyer 博士组成。细胞储存设施由一个可容纳 25,000 个小瓶的液氮罐组成。中心研究人员储存来自患有罕见遗传病的患者和亲属的成纤维细胞和淋巴母细胞系。它有所有细胞系的计算机化日志，供研究人员跟踪 细胞系的位置和用途。共享设备组件包含几台大型设备，包括用于基因分型和测序的 ABI Prism 310 遗传分析仪、用于检测和分析单碱基对突变的 Varian Helix dHPLC 系统、新型 Mini-Med 胶片处理器。它还配备了多台基本实验室设备，如超高速离心机、轨道摇床、干燥箱、X 射线交联器、凝胶干燥器、真空浓缩器、蒸馏水、去离子器、分光光度计、Bio-Rad Gel Doc 2000和 Bio-Rad Gene Pulser II。细胞存储和共享设备组件由儿科研究副教授 Thomas Cotner 博士维护和协调。共有 27 名中心研究人员和 37 个项目被列出使用该核心的各个组件。