B-CLL Proteins Quantified by Mass Spectrometry

通过质谱法定量 B-CLL 蛋白质

• 批准号: 6784208
• 负责人: David Robert Barnidge
• 金额: $ 13.49万
• 依托单位: MAYO CLINIC ROCHESTER
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-08-01 至 2005-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6784208
• 关键词: B lymphocyte; affinity chromatography; biomarker; chronic lymphocytic leukemia; clinical research; cytogenetics; electrospray ionization mass spectrometry; flow cytometry; gene expression; human subject; infrared spectrometry; ion cyclotron resonance spectrometry; liquid chromatography mass spectrometry; mass spectrometry; messenger RNA; microarray technology; neoplasm /cancer diagnosis; neoplasm /cancer genetics; neoplasm /cancer invasiveness; neoplasm /cancer therapy; oncoproteins; polymerase chain reaction; prognosis; protein purification; protein quantitation /detection; proteomics

DESCRIPTION (provided by applicant): The proposed K25 mentored quantitative research career development award will allow Dr. David R. Barnidge to learn how to apply mass spectrometry and molecular biology to the identification and quantification of proteins associated with B cell chronic lymphocytic leukemia (B-CLL). B-CLL is the most common form of leukemia in the adult population of the United States and treatment of the disease is complicated by the diverse response of patients to treatment. The proposed work seeks to provide confirmation and quantification of possible biomarkers specific for B-CLL, aiding clinicians in selecting the proper course of treatment. The proteome of normal and B-CLL B cells will be studied by 2D-LC/MS/MS and gene microarray analysis to look for similarities and differences between expressed genes and protein product. Quantification of specific protein biomarkers will be done by LC/MS/MS while the population of leukemic B cells will be monitored using the accurate mass of affinity purified Ig light chains determined by FTICR MS. The future goals of the candidate are to combine mass spectrometry and molecular biology in the pursuit of quantitative analysis of gene products related to cancer. To this end the proposed experiments incorporate the candidate's knowledge of quantitative analysis, mass spectrometry, and protein chemistry and merge them with molecular biology and clinical biomedicine, areas where the candidate has limited knowledge. The mentors for this proposal are eager to assist the candidate in developing his knowledge of FTICR MS, gene microarray analysis, and quantitative PCR as part of his training in the Mayo Proteomics Research Center and the Mayo Department of Immunology. As part of this proposal the candidate will take courses offered in the Mayo Graduate School on Techniques in Molecular Biology, Human Cytogenetics, and Immunology.

描述（由申请人提供）：拟议的K25指导的定量研究职业发展奖将使David R. Barnidge博士能够学习如何将质谱和分子生物学应用于与B细胞慢性淋巴细胞性白血病（B-CLL）相关的蛋白质鉴定和定量。 B-CLL是美国成年人口中白血病最常见的形式，患者对治疗的多种反应使该疾病的治疗变得复杂。 拟议的工作旨在提供针对B-CLL的可能生物标志物的确认和量化，这是帮助临床医生选择适当治疗过程的。 正常和B-CLL B细胞的蛋白质组将通过2D-LC/MS/MS/MS和基因微阵列分析研究，以寻找表达基因和蛋白质产物之间的相似性和差异。 特异性蛋白质生物标志物的定量将由LC/MS/MS进行，而白血病B细胞的群体将使用由FTICR MS确定的准确纯化的IG光链进行监测。 候选人的未来目标是将质谱和分子生物学结合起来，以追求与癌症相关的基因产物的定量分析。 为此，提出的实验结合了候选人的定量分析，质谱和蛋白质化学的知识，并将其与分子生物学和临床生物医学合并，该地区的知识有限。 该提案的导师渴望帮助候选人发展他对FTICR MS，Gene Microaray分析和定量PCR的了解，这是他在Mayo蛋白质组学研究中心和Mayo免疫学系培训的一部分。 作为该提案的一部分，候选人将在Mayo研究生院接受有关分子生物学，人类细胞遗传学和免疫学技术的课程。