Novel, Noninvasive Biomarker of Fruit & Vegetable Intake

新型非侵入性水果生物标志物

• 批准号: 6745587
• 负责人: SUSAN T MAYNE
• 金额: $ 29.67万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-05-08 至 2006-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6745587
• 关键词: Raman spectrometry; biomarker; biopsy; carotenoids; clinical research; fruit; high performance liquid chromatography; human middle age (35-64); human subject; nutrient intake activity; questionnaires; skin; technology /technique development; tomato; vegetables; young adult human (21-34)

Epidemiologic studies of fruits/vegetables and chronic disease have been limited by the lack of non-invasive objective biomarkers of dietary intake/dietary adherence to interventions. Blood concentrations of carotenoids are considered the best biological markers of fruit and vegetable intake, but require venipuncture and are prohibitively expensive for large cohort studies and intervention trials. Carotenoids are found in many human tissues including skin, and skin concentrations of carotenoids (from dermal biopsies) have been shown to be strongly and significantly correlated with plasma concentrations of the same carotenoids (e.g., beta-carotene, r=0.75). We propose to adapt an existing technology, Raman spectroscopy, for the assessment of the carotenoid status of human tissues in vivo as a biomarker of fruit and vegetable intake. More specifically, we propose to (i) refine our current instrument such as to have a rugged, portable device for rapidly assessing carotenoid status ill human skin in vivo. Once our protype instrument is appropriately refined, we then propose to (ii) evaluate the reproducibility, intra-subject variability, and inter-subject variability in dermal cartenoid levels as estimated by Raman spectroscopy at one time and over time. Upon completion of this aim, our final aim is to (iii) determine the correlation between carotenoid status as assessed by Raman spectroscopy and other measures of carotenoid status including dietary intake of fruits/vegetables and specific carotenoids, plasma carotenoid concentrations and carotenioid concentrations in dermal biopsies. This promising technology is currently being developed/refined for the assessment of carotenoids in human retinal tissue (National Eye Institute); the current proposal would greatly broaden the potential use of this noninvasive technology for cancer epidemiology and prevention studies. Raman spectroscopic technology has tremendous potential as a breakthrough method for rapid screening of carotenoid levels in large populations, and development and validation are critically required to move this technology into human population research and surveillance.

由于缺乏饮食摄入/饮食遵守干预措施的非侵入性客观生物标志物，水果/蔬菜和慢性病的流行病学研究受到限制。类胡萝卜素的血液浓度被认为是果实和蔬菜摄入量的最佳生物学标志物，但需要静脉穿刺，并且对于大型队列研究和干预试验而言，其昂贵。在包括皮肤在内的许多人体组织中发现了类胡萝卜素，皮肤浓度（来自真皮活检）已显示出强烈的，并且 与同一类胡萝卜素的血浆浓度显着相关（例如β-胡萝卜素，r = 0.75）。我们建议适应现有技术，拉曼光谱法，以评估体内人体组织的类胡萝卜素状态，作为水果和蔬菜摄入的生物标志物。更具体地说，我们建议（i）完善当前的仪器，例如拥有一个坚固的便携式设备，用于快速评估体内类胡萝卜素状态不适。 一旦我们的原型仪器得到适当完善，我们就建议（ii）评估可重复性， 根据拉曼光谱法估计，受试者内的变异性和皮肤肉体肉质水平的受试者间变异性一次和随着时间的流逝。完成此目标后，我们的最终目的是（iii）确定由拉曼光谱法评估的类胡萝卜素状态与类胡萝卜素状态的其他度量，包括水果/蔬菜的饮食摄入量和特定的类胡萝卜素，血浆类胡萝卜素的浓度和类胡萝卜素的浓度。目前正在开发/完善这项有希望的技术以评估 人类视网膜组织中的类胡萝卜素（国家眼科研究所）；当前的提案将大大扩展这种非侵入性技术在癌症流行病学和预防研究中的潜在使用。拉曼光谱技术具有巨大的潜力，作为大量人群中类胡萝卜素水平快速筛查的突破性方法，并且需要将这项技术转移到人群研究和监视中。