Novel, Noninvasive Biomarker of Fruit & Vegetable Intake

新型非侵入性水果生物标志物

• 批准号: 6745587
• 负责人: SUSAN T MAYNE
• 金额: $ 29.67万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-05-08 至 2006-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6745587
• 关键词: Raman spectrometry; biomarker; biopsy; carotenoids; clinical research; fruit; high performance liquid chromatography; human middle age (35-64); human subject; nutrient intake activity; questionnaires; skin; technology /technique development; tomato; vegetables; young adult human (21-34)

Epidemiologic studies of fruits/vegetables and chronic disease have been limited by the lack of non-invasive objective biomarkers of dietary intake/dietary adherence to interventions. Blood concentrations of carotenoids are considered the best biological markers of fruit and vegetable intake, but require venipuncture and are prohibitively expensive for large cohort studies and intervention trials. Carotenoids are found in many human tissues including skin, and skin concentrations of carotenoids (from dermal biopsies) have been shown to be strongly and significantly correlated with plasma concentrations of the same carotenoids (e.g., beta-carotene, r=0.75). We propose to adapt an existing technology, Raman spectroscopy, for the assessment of the carotenoid status of human tissues in vivo as a biomarker of fruit and vegetable intake. More specifically, we propose to (i) refine our current instrument such as to have a rugged, portable device for rapidly assessing carotenoid status ill human skin in vivo. Once our protype instrument is appropriately refined, we then propose to (ii) evaluate the reproducibility, intra-subject variability, and inter-subject variability in dermal cartenoid levels as estimated by Raman spectroscopy at one time and over time. Upon completion of this aim, our final aim is to (iii) determine the correlation between carotenoid status as assessed by Raman spectroscopy and other measures of carotenoid status including dietary intake of fruits/vegetables and specific carotenoids, plasma carotenoid concentrations and carotenioid concentrations in dermal biopsies. This promising technology is currently being developed/refined for the assessment of carotenoids in human retinal tissue (National Eye Institute); the current proposal would greatly broaden the potential use of this noninvasive technology for cancer epidemiology and prevention studies. Raman spectroscopic technology has tremendous potential as a breakthrough method for rapid screening of carotenoid levels in large populations, and development and validation are critically required to move this technology into human population research and surveillance.

水果/蔬菜和慢性病的流行病学研究因缺乏饮食摄入/饮食干预坚持的非侵入性客观生物标志物而受到限制。类胡萝卜素的血液浓度被认为是水果和蔬菜摄入量的最佳生物标志物，但需要静脉穿刺，并且对于大型队列研究和干预试验来说费用过高。类胡萝卜素存在于包括皮肤在内的许多人体组织中，并且类胡萝卜素（来自真皮活检）的皮肤浓度已被证明是强烈和 与相同类胡萝卜素（例如 β-胡萝卜素，r=0.75）的血浆浓度显着相关。我们建议采用现有的拉曼光谱技术来评估体内人体组织的类胡萝卜素状态，作为水果和蔬菜摄入量的生物标志物。更具体地说，我们建议（i）改进我们现有的仪器，例如拥有一种坚固耐用的便携式设备，用于快速评估体内人类皮肤的类胡萝卜素状态。 一旦我们的原型仪器得到适当的改进，我们就会建议（ii）评估再现性， 通过拉曼光谱一次和一段时间内估计的真皮类胡萝卜素水平的受试者内部变异性和受试者间变异性。完成这一目标后，我们的最终目标是 (iii) 确定拉曼光谱评估的类胡萝卜素状态与其他类胡萝卜素状态测量之间的相关性，包括水果/蔬菜和特定类胡萝卜素的膳食摄入量、血浆类胡萝卜素浓度和真皮中类胡萝卜素浓度活检。这项有前景的技术目前正在开发/完善，用于评估 人类视网膜组织中的类胡萝卜素（国家眼科研究所）；目前的提案将大大拓宽这种非侵入性技术在癌症流行病学和预防研究中的潜在用途。拉曼光谱技术作为快速筛查大量人群中类胡萝卜素水平的突破性方法具有巨大的潜力，并且迫切需要开发和验证将该技术应用于人群研究和监测。