Analysis of Nitric Oxide During Meridian Practices

经络练习过程中一氧化氮的分析

• 批准号: 6838335
• 负责人: SHENG-XING MA
• 金额: $ 20.06万
• 依托单位: LUNDQUIST INSTITUTE FOR BIOMEDICAL INNOVATION AT HARBOR-UCLA MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-29 至 2008-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6838335
• 关键词: acupuncture /acupressure; alternative medicine; biosynthesis; clinical research; electrical conductance; electrical impedance; electrodes; electrostimulus; heat stimulus; human subject; inhibitor /antagonist; meditation; nitric oxide

DESCRIPTION (provided by applicant): The meridian system is the central theory and guiding principle for therapies related to mind-body interactions such as acupuncture, Qi Gong and meditation. Investigators have demonstrated in both humans and animals that acupoints/meridians possess characteristics of low electric resistance. We have found that nitric oxide (NO) contents and neuronal NO synthase expressions are consistently increased in three meridian skin regions in rats. Our recent studies reveal that NO contents are increased over PC 4 to 7 containing 4 acupoints and tended to increase in BL 56 to 57 along the region with two acupoints compared to nonmeridian control in humans. Consistent with the postulated role of nonenzymatic NO generation, enhanced NO levels in the acupoints/meridians are reduced by using sodium hypochlorite to reduce bacteria on skin surface. Furthermore, preliminary data show that NO contents are increased over PC acupoints and meridian during meditation practice. I hypothesize that 1) Nonenzymatic NO production and neuronal NO synthesis contribute to enhanced NO generation in acupoint/meridians; and 2) Meridian practices induce NO generation which mediates chemical-electrical activity for the biophysical characteristics of acupoints/meridians. In view of the critical importance and unexplored territory of the meridian system, our major aims are: 1) Define whether local NO levels (NOx- concentrations) are selectively increased in the acupoints and/or in the meridian regions; 2) Determine if nonenzymatic NO generation and/or neuronal NO synthesis contribute to enhanced NO levels in acupoints/meridians; 3) Identify if electrical and temperature stimulation induces NO production in acupoints/meridians paralleled to low electrical resistance; 4) Examine whether acupuncture induces NO production/release along dual directions of meridian pathways; and 5) Investigate whether Qi Gong and/or meditation practices predominantly increase NO generation in the acupoints and meridians. Quantification of NO metabolites collected from the skin surface of acupoints and meridian lines with a special device will be integrated with physiological/pharmacological manipulations during meridian practices to test the hypotheses in humans. The results should advance our understanding of the roles of endogenous NO generation/release in responses to acupuncture, Qi Gong and meditation practices m the acupoints/meridians, and yield new insights regarding the effects of non-enzymatic NO generation and neuronal NO synthesis on chemical-electrical activity of the low skin resistance function of meridians.

描述（由申请人提供）：子午系统是与身心相互作用有关的疗法的中心理论和指导原则，例如针灸，气锣和冥想。研究人员在人类和动物中都证明，尖端/子午线具有低电阻的特征。我们发现，一氧化氮（NO）含量（NO）和神经元NO合酶表达在大鼠的三个子午皮肤区域始终增加。我们最近的研究表明，与人类的非占控制者相比，在该地区的PC 4到7中，PC 4至7中没有任何内容物增加，并倾向于增加BL 56至57，而有两个兆头。与非酶无产生的假设作用一致，通过使用次氯酸钠来减少皮肤表面上的细菌，可以降低尖端/子午线中没有水平。此外，初步数据表明，在冥想练习期间，PC螺旋线和子午线的内容没有增加。我假设1）非酶无生产和神经元无合成有助于增强穴位/子午线的产生； 2）子午线实践不会引起任何介导的化学电活动的产生，以促成穴位/子午线的生物物理特征。 鉴于子午系统的重要性和未开发的领域，我们的主要目的是：1）定义兆头和/或子午线区域中是否有选择地增加局部无水平（NOX-浓度）； 2）确定非酶无产生和/或神经元无合成是否有助于提高穴位/子午线的NO水平； 3）确定电刺激和温度刺激是否诱导与低电阻平行的穴位/子午线中没有产生； 4）检查针灸是否沿着子午途径的双向诱导/释放没有产生/释放； 5）调查智锣和/或冥想实践是否主要增加兆头和子午线的产生。在子午线实践期间，将与特殊装置的穴位表面和子午线线的皮肤表面收集的无代谢产物进行定量，以测试人类的假设。结果应提高我们对内源性无产生/释放在对针灸，Qi锣和冥想中的作用的理解，从而在尖端/子午线中实践，并就非酶无产生和神经元的NO合成对子属耐药性功能的化学效果的非酶无产生和神经元合成的影响产生新的见解。