Core-- Administration

核心——行政管理

• 批准号: 6853157
• 负责人: Joseph Zabner
• 金额: $ 7.25万
• 依托单位: UNIVERSITY OF IOWA
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-04-01 至 2009-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6853157
• 关键词: biomedical facility; cell bank /registry; cell biology; cell line; cystic fibrosis; disease /disorder model; electrophysiology; genotype; human tissue; method development; model design /development; morphology; respiratory epithelium; tissue /cell culture; transfection

The In Vitro Models and Cell Culture Core is established as a resource for all projects in the PPG. The specific aims are: 1. Acquisition of normal and CF tissue. 2. Generation of in vitro models of human airway epithelia. 3. Characterization of in vitro models and native epithelia. a. Electrophysiologic analysis. b. Morphologic evaluation. c. Genotyping of CF cells. 4. Research and development of new methods and models for the study of gene transfer, airway epithelial, cell biology, and submucosal glands. 5. Production of cultured cells for investigators in the PPG. 6. Development of a cryogenic bank of airway epithelial cells. 7. Teaching investigators at the University of Iowa and other institutions the methods for developing in vitro model systems. 8. Record keeping relevant to tissue acquisition and cell culture. 9. Obtaining approval from regulatory committees and record keeping for cell and animal studies. 10. Provision of cells, plasmids, and viruses to investigators at other institutions. This Core brings the expertise of a highly motivated and technically sophisticated staff to the benefit of the PPG investigators. As a result, it allows PPG investigators to focus their unique expertise on questions of high relevance to CF gene transfer.

体外模型和细胞培养核心被确定为所有项目的资源 ppg。具体目的是： 1。采集正常和CF组织。 2。人类气道上皮的体外模型产生。 3。体外模型和天然上皮的表征。 一个。电生理分析。 b。形态评估。 c。 CF细胞的基因分型。 4。用于研究基因转移，气道上皮，细胞生物学和粘膜腺的新方法和模型的研究和开发。 5。在PPG中为研究人员生产培养细胞。 6。生气道上皮细胞的低温库。 7.爱荷华大学和其他机构的调查人员教授方法 开发体外模型系统。 8。记录与组织采集和细胞培养有关。 9。获得监管委员会的批准，并记录细胞和动物的记录 研究。 10。向其他机构的研究人员提供细胞，质粒和病毒。 这个核心将一位具有积极进取和技术成就的员工的专业知识带给 PPG调查人员的好处。结果，它使PPG调查人员可以集中精力 关于与CF基因转移高相关性问题的独特专业知识。