Dynamic MRI and MRS studies of focal neural activation

局灶性神经激活的动态 MRI 和 MRS 研究

• 批准号: 6701757
• 负责人: ROLF GRUETTER
• 金额: $ 34.61万
• 依托单位: UNIVERSITY OF MINNESOTA
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-04-10 至 2007-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6701757
• 关键词: Krebs' cycle; aspartate; beta hydroxybutyrate; bioenergetics; bioimaging /biomedical imaging; brain circulation; brain metabolism; clinical research; functional magnetic resonance imaging; glia; glucose metabolism; glucose transport; glycogen; glycogenolysis; human subject; laboratory rat; malates; neural transmission; neurochemistry; neurons; nuclear magnetic resonance spectroscopy; phosphorylases; pyruvate dehydrogenase; squirrel; Krebs' cycle; aspartate; beta hydroxybutyrate; bioenergetics; bioimaging /biomedical imaging; brain circulation; brain metabolism; clinical research; functional magnetic resonance imaging; glia; glucose metabolism; glucose transport; glycogen; glycogenolysis; human subject; laboratory rat; malates; neural transmission; neurochemistry; neurons; nuclear magnetic resonance spectroscopy; phosphorylases; pyruvate dehydrogenase; squirrel

DESCRIPTION (provided by applicant): In contrast to the hemodynamic and glucose/oxygen metabolic consequences of altered electrical activity, the neurochemical, neuroenergetic and metabolic basis of brain activation at the neuroglial level is not as well researched in vivo, It is our long-term objective to obtain a comprehensive assessment of cerebral carbohydrate metabolism as it relates to increased brain activity and neuronal/glial compartmentation in the intact brain. Our central hypothesis is that glutamate neurotransmission represents a fraction of total brain glucose consumption and that the malate-aspartate shuttle is a major rate-determining mechanism in oxidative brain glucose metabolism. The overall strategy is to measure glial and neuronal energy metabolism, malate-aspartate shuttle (MAS) activity, brain glycogen metabolism and glucose transport in rats, hibernating squirrels and humans using an emerging new tool, state-of-the-art 13C and 1H NMR spectroscopy at ultra-high magnetic fields, combined with functional MR imaging. The project represents the continuation of our ongoing effort, the outcome of which will be to provide an unprecedented comprehensive understanding of the neurochemical basis of brain function in vivo at the cellular level, with the potential to characterize disease progression long before structural changes are evident in many neurodegenerative and other diseases of the brain, where glutamate has been implicated.

描述（由申请人提供）： 与改变电活动改变的血液动力学和葡萄糖/氧代谢后果相反，在神经群水平上的神经化学，神经化学和代谢基础的神经化学，神经能和代谢基础并不是对体内研究的良好研究，这是我们的长期客观的研究，即获得脑部脑循环/脑膜化的脑膜化和脑膜化的全面评估。在完整的大脑中。我们的中心假设是，谷氨酸神经传递代表了总脑葡萄糖消耗的一部分，而苹果酸 - 天冬氨酸班车是氧化性脑葡萄糖代谢的主要速率确定机制。总体策略是测量大鼠的神经胶质和神经元能量代谢，牛酸天冬氨酸班车（MAS）活性，大脑糖原代谢和葡萄糖的转运，冬眠的松鼠和人类使用Emerging New工具，最先进的工具，最先进的13C和1H NMR光谱型在Ultra-High-High-High-High-High磁性领域，并具有功能的功能。该项目代表了我们持续不断的努力的延续，其结果将是在细胞水平上对体内的大脑功能的神经化学基础提供前所未有的全面理解，并且在许多神经退行性和其他大脑的疾病中显而易见的是疾病进展的潜力，在结构变化之前很明显。