iDQC MR Imaging of Tumor Pathophysiology

肿瘤病理生理学的 iDQC MR 成像

• 批准号: 6622112
• 负责人: JIANHUI ZHONG
• 金额: $ 14.4万
• 依托单位: UNIVERSITY OF ROCHESTER
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-04-05 至 2005-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6622112
• 关键词: angiogenesis; bioimaging /biomedical imaging; biophysics; blood vessels; diagnosis design /evaluation; disease /disorder model; image enhancement; immunocytochemistry; laboratory mouse; magnetic resonance imaging; morphometry; necrosis; neoplasm /cancer classification /staging; neoplasm /cancer diagnosis; neoplastic process; phantom model; respiratory oxygenation; statistics /biometry; technology /technique development

DESCRIPTION (provided by applicant): MR imaging has been used extensively in clinical practice for tumor diagnosis. A wide variety of functional MRI techniques have also been developed to non-invasively assess tumor flow and oxygenation. In particular dynamic contrast enhancement (DCE) MRI has been shown to correlate well with tumor angiogenesis. BOLD effects have also been used to measure changes due to blood oxygenation and flow in tumor studies. With careful experimental design, BOLD effects may also provide quantitative information about oxygen saturation. Recently we have been developing a novel MR imaging technique based on intermolecular double-quantum coherences (iDQCs), which is inherently more sensitive to some physical processes relevant to tumor physiological characterization. In this application, we propose three different types of MRI measurements based on iDQCs, which will provide more sensitive and specific characterization of tumors in terms of: (1) oxygenation; (2) spatial distributions of microvessels at spatial resolutions far below conventional MRI; and (3) necrotic fraction. In the this phase, iDQC imaging methods suitable for tumor characterization will be developed and compared with conventional SQC MRI. Experimental murine tumor models, MCA-35 and MCA-4, which have been extensively characterized by the co-PI, Dr. Fenton, with immunohistochemical techniques, will be used for iDQC MR imaging on a 9.4T MR scanner. Histograms of physiological parameters measured with iDQC imaging will be compared to results from immunohistochemical measurements and conventional SQC MR/. Correlation will be sought in selected regions in the tumor core and periphery, as well as normal tissues. In the next phase, methods for multispectral analysis of images will be developed to address the multi-dimensional nature of tumor pathophysiology with integrated data from oxygen saturation, microvascular distribution, and tumor necrotic fraction measurements. Spatial correlation between MRI and immunohistochemical images for quantifying blood vessel distribution and oxygen saturation will also be pursued in this phase. Response to increased oxygen and effects of radiation therapy on tumor oxygenation and angiogenesis will be studied to validate the utility of new iDQC imaging techniques. The novel iDQC MR imaging proposed in this study will provide enhanced sensitivity and specificity for measurements based on the BOLD effect, and provide a new measurement for tumor vascularity complementary to conventional DCE techniques. Since the iDQC techniques proposed in this application do not involve changes in hardware, they can be translated into clinical applications relatively quickly once the methods are carefully validated.

描述（由申请人提供）： MR成像已在临床实践中广泛用于肿瘤诊断。 还开发了多种功能性MRI技术 非侵入性评估肿瘤流量和氧合。特别是动态 对比度增强（DCE）MRI已显示与肿瘤良好相关 血管生成。大胆的效果也已用于测量由于血液而导致的变化 肿瘤研究中的氧合和流量。仔细的实验​​设计，大胆 效果还可以提供有关氧饱和度的定量信息。 最近，我们一直在开发一种基于新颖的MR成像技术 分子间双量子相干（IDQC），它本质上是更多 对某些与肿瘤生理有关的物理过程敏感 表征。在此应用中，我们提出了三种不同类型的MRI 基于IDQC的测量值，该测量将提供更敏感和更具体的 肿瘤的表征：（1）氧合； （2）空间 在空间分辨率上的微血管的分布远低于常规分辨率 MRI; （3）坏死分数。在此阶段，IDQC成像方法 适用于肿瘤表征并将其与 常规的SQC MRI。实验性鼠肿瘤模型，MCA-35和MCA-4， 由Co-Pi广泛特征的Fenton博士， 免疫组织化学技术将用于9.4T MR上的IDQC MR成像 扫描器。用IDQC成像测量的生理参数的直方图 将与免疫组织化学测量结果和 常规SQC MR/。将在选定地区寻求相关性 肿瘤核心和周围以及正常组织。在下一个阶段，方法 为了进行多光谱分析图像，以解决 肿瘤病理生理学的多维性质以及来自 氧饱和度，微血管分布和肿瘤坏死部分 测量。 MRI与免疫组织化学图像之间的空间相关性 用于量化血管分布和氧饱和度也将是 在此阶段追求。对氧气增加和辐射的影响的反应 将研究有关肿瘤氧合和血管生成的治疗 新的IDQC成像技术的实用性。小说IDQC MR成像提出 这项研究将为测量提供增强的灵敏度和特异性 基于大胆的效果，并为肿瘤血管提供新的测量 互补的常规DCE技术。由于IDQC技术 在此应用程序中提出的不涉及硬件的更改，它们可以是 一旦方法是相对较快地转换为临床应用 经过仔细的验证。