iDQC MR Imaging of Tumor Pathophysiology

肿瘤病理生理学的 iDQC MR 成像

• 批准号: 6622112
• 负责人: JIANHUI ZHONG
• 金额: $ 14.4万
• 依托单位: UNIVERSITY OF ROCHESTER
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-04-05 至 2005-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6622112
• 关键词: angiogenesis; bioimaging /biomedical imaging; biophysics; blood vessels; diagnosis design /evaluation; disease /disorder model; image enhancement; immunocytochemistry; laboratory mouse; magnetic resonance imaging; morphometry; necrosis; neoplasm /cancer classification /staging; neoplasm /cancer diagnosis; neoplastic process; phantom model; respiratory oxygenation; statistics /biometry; technology /technique development

DESCRIPTION (provided by applicant): MR imaging has been used extensively in clinical practice for tumor diagnosis. A wide variety of functional MRI techniques have also been developed to non-invasively assess tumor flow and oxygenation. In particular dynamic contrast enhancement (DCE) MRI has been shown to correlate well with tumor angiogenesis. BOLD effects have also been used to measure changes due to blood oxygenation and flow in tumor studies. With careful experimental design, BOLD effects may also provide quantitative information about oxygen saturation. Recently we have been developing a novel MR imaging technique based on intermolecular double-quantum coherences (iDQCs), which is inherently more sensitive to some physical processes relevant to tumor physiological characterization. In this application, we propose three different types of MRI measurements based on iDQCs, which will provide more sensitive and specific characterization of tumors in terms of: (1) oxygenation; (2) spatial distributions of microvessels at spatial resolutions far below conventional MRI; and (3) necrotic fraction. In the this phase, iDQC imaging methods suitable for tumor characterization will be developed and compared with conventional SQC MRI. Experimental murine tumor models, MCA-35 and MCA-4, which have been extensively characterized by the co-PI, Dr. Fenton, with immunohistochemical techniques, will be used for iDQC MR imaging on a 9.4T MR scanner. Histograms of physiological parameters measured with iDQC imaging will be compared to results from immunohistochemical measurements and conventional SQC MR/. Correlation will be sought in selected regions in the tumor core and periphery, as well as normal tissues. In the next phase, methods for multispectral analysis of images will be developed to address the multi-dimensional nature of tumor pathophysiology with integrated data from oxygen saturation, microvascular distribution, and tumor necrotic fraction measurements. Spatial correlation between MRI and immunohistochemical images for quantifying blood vessel distribution and oxygen saturation will also be pursued in this phase. Response to increased oxygen and effects of radiation therapy on tumor oxygenation and angiogenesis will be studied to validate the utility of new iDQC imaging techniques. The novel iDQC MR imaging proposed in this study will provide enhanced sensitivity and specificity for measurements based on the BOLD effect, and provide a new measurement for tumor vascularity complementary to conventional DCE techniques. Since the iDQC techniques proposed in this application do not involve changes in hardware, they can be translated into clinical applications relatively quickly once the methods are carefully validated.

描述（由申请人提供）： 磁共振成像已广泛应用于临床实践中的肿瘤诊断。 还开发了多种功能性 MRI 技术 非侵入性评估肿瘤流量和氧合。特别是动态 对比增强 (DCE) MRI 已被证明与肿瘤密切相关 血管生成。 BOLD 效果也被用来测量血液引起的变化 肿瘤研究中的氧合和血流。通过精心的实验设计，BOLD 效应还可以提供有关氧饱和度的定量信息。 最近，我们一直在开发一种基于 分子间双量子相干性（iDQC），本质上更 对一些与肿瘤生理相关的物理过程敏感 表征。在此应用中，我们提出了三种不同类型的 MRI 基于 iDQC 的测量，这将提供更灵敏、更具体的测量 肿瘤的特征包括：（1）氧合； (2)空间 微血管的空间分辨率远低于传统分布 核磁共振成像； (3)坏死分数。在此阶段，iDQC 成像方法 将开发适合肿瘤表征的方法并与 常规 SQC MRI。实验性小鼠肿瘤模型 MCA-35 和 MCA-4， 共同 PI Fenton 博士对这些问题进行了广泛的描述 免疫组织化学技术，将用于 9.4T MR 上的 iDQC MR 成像 扫描器。使用 iDQC 成像测量的生理参数直方图 将与免疫组织化学测量的结果进行比较 传统SQC MR/。将在选定的地区寻求相关性 肿瘤核心和周围，以及正常组织。下一阶段，方法 将开发用于图像多光谱分析的技术来解决 肿瘤病理生理学的多维性质与综合数据 氧饱和度、微血管分布和肿瘤坏死分数 测量。 MRI 和免疫组织化学图像之间的空间相关性 用于量化血管分布和氧饱和度也将 这一阶段所追求的。对增加氧气和辐射影响的反应 将研究肿瘤氧合和血管生成的治疗方法以验证 新 iDQC 成像技术的实用性。提出的新型 iDQC MR 成像 这项研究将提高测量的灵敏度和特异性 基于BOLD效应，为肿瘤血管分布提供新的测量方法 对传统 DCE 技术的补充。由于 iDQC 技术 本申请中提出的不涉及硬件的改变，它们可以是 一旦这些方法被采用，就可以相对较快地转化为临床应用 仔细验证。