Noninvsive Imaging of Chorio-retinal Oxygen Tension

脉络膜视网膜氧张力的无创成像

• 批准号: 6673945
• 负责人: Mahnaz Shahidi
• 金额: $ 15.59万
• 依托单位: UNIVERSITY OF ILLINOIS AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-08-01 至 2006-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6673945
• 关键词: hypoxia; imaging /visualization /scanning; laboratory rat; measurement; noninvasive diagnosis; oxygen tension; retina circulation; statistics /biometry; technology /technique development

DESCRIPTION (provided by applicant): Retinal hypoxia is implicated in the development of many major and common blinding human eye diseases, such as diabetic retinopathy and age-related macular degeneration. The metabolic heath and function of retinal cells is based on the availability of nutrients and oxygen. Two vasculature beds, the blood vessels within the retinal tissue and the choroid, a layer of vascular tissue underneath the retinal tissue, supply oxygen to the retina. Currently, the relative contribution of these vasculatures to the oxygenation of the retinal tissue in health and disease has not been adequately studied, and the role of oxygen in the development of retinal diseases, and their associated vascular pathologies, is not well understood. Therefore, technologies that allow understanding, diagnosis and treatment of diseases in which oxygen is implicated are greatly needed. Although techniques for measurement of blood flow in the large retinal blood vessels are available, they provide only an indirect measure of retinal oxygenation. Invasive methods for retinal oxygen tension measurements that utilize oxygen-sensitive microelectrodes are also available, but not clinically applicable. Recently, measurement of oxygen tension in the retina, with the use of a phosphorescence imaging technique, has been demonstrated. However, due to lack of depth discrimination, this technique does not allow discrete measurement of oxygen tension in the chorio-retinal vasculature and is limited for measurements in the microvasculature of the retina, where the first indication of disease is known to occur. In the proposed research study, we intend to overcome the limitations of the available techniques by establishing a novel system that combines optical section retinal imaging with oxygen-sensitive probe phosphorescence imaging for noninvasive and quantitative differentiation of oxygen tension in the chorio-retinal vasculature of the eye. The specific aims are to develop the system, establish its reliability and reproducibility, determine normal ranges, and detect changes in oxygen tension across the depth of the retinal tissue. Once refined, the system will serve as a valuable tool for three-dimensional retinal oxygen tension imaging. Such imaging will prompt responses to a vast number of eminent research questions on disease-related oxygen dynamics in retinal, choroidal, and intra-retinal vasculatures and thereby, improve diagnosis and understanding of the pathophysiology of retinal diseases.

描述（由申请人提供）：视网膜缺氧与许多主要和常见的致盲人类眼部疾病的发生有关，例如糖尿病性视网膜病变和年龄相关性黄斑变性。视网膜细胞的代谢健康和功能取决于营养物质和氧气的可用性。 两个脉管床，即视网膜组织内的血管和脉络膜（视网膜组织下方的一层血管组织），为视网膜提供氧气。 目前，这些脉管系统对健康和疾病状态下视网膜组织氧合的相对贡献尚未得到充分研究，并且氧在​​视网膜疾病发展中的作用及其相关的血管病理学尚未得到充分了解。因此，非常需要能够了解、诊断和治疗与氧气有关的疾病的技术。 尽管可以使用测量大视网膜血管中的血流量的技术，但它们仅提供视网膜氧合的间接测量。 利用氧敏感微电极进行视网膜氧分压测量的侵入性方法也是可用的，但不适用于临床。 最近，已经证明使用磷光成像技术测量视网膜中的氧张力。 然而，由于缺乏深度辨别，该技术不允许离散测量脉络膜视网膜脉管系统中的氧张力，并且仅限于视网膜微脉管系统中的测量，其中已知疾病的第一个迹象会发生。 在拟议的研究中，我们打算通过建立一种新颖的系统来克服现有技术的局限性，该系统将光学切片视网膜成像与氧敏感探针磷光成像相结合，以无创和定量区分脉络膜视网膜脉管系统中的氧张力。眼睛。 具体目标是开发该系统，建立其可靠性和再现性，确定正常范围，并检测视网膜组织深度的氧张力变化。 一旦完善，该系统将成为三维视网膜氧张力成像的有价值的工具。这种成像将促进对视网膜、脉络膜和视网膜内脉管系统中与疾病相关的氧动力学的大量重要研究问题的回答，从而改善对视网膜疾病病理生理学的诊断和理解。