Spatiotemporal Brain Imaging: Microscopic & System Level

时空脑成像：显微镜

• 批准号: 6495886
• 负责人: ANDERS M DALE
• 金额: $ 206.38万
• 依托单位: MASSACHUSETTS GENERAL HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-05 至 2007-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6495886
• 关键词: Macaca mulatta; bioengineering /biomedical engineering; biomedical equipment development; biophysics; brain electrical activity; brain imaging /visualization /scanning; confocal scanning microscopy; image enhancement; image processing; laboratory rat; magnetic resonance imaging; migraine; neural inhibition; noninvasive diagnosis; optical tomography; somesthetic sensory cortex; spreading cortical depression; stroke; temporal lobe /cortex; vibrissae; visual cortex; Macaca mulatta; bioengineering /biomedical engineering; biomedical equipment development; biophysics; brain electrical activity; brain imaging /visualization /scanning; confocal scanning microscopy; image enhancement; image processing; laboratory rat; magnetic resonance imaging; migraine; neural inhibition; noninvasive diagnosis; optical tomography; somesthetic sensory cortex; spreading cortical depression; stroke; temporal lobe /cortex; vibrissae; visual cortex

Description (provided by application): The last decade has brought revolutionary new techniques allowing visualization of the working brain in humans at the systems level. However, a large gap remains between the spatiotemporal resolution of tomographic techniques (fMRI, PET), and the circuit level where animal studies permit mechanistic neural models. It is the overall goal of this proposal to develop an integrated suite of technologies to bridge this critical gap. Two interrelated themes are found throughout this proposal: (1) to improve the spatial and temporal resolution of non-invasive technologies, which will enable direct imaging of discrete (e g column and laminar level) neural units which bridge the systems and cellular levels and (2) to clarify the mechanisms which relate the biophysics of neuronal activity "observables" in our imaging measurements. The two key technologies to be investigated are: (1) extremely high resolution MRI and fMRI, using very high strength gradients, phased-array coils, and other advances at 3T and 7T non-human primates, and 9.4T rats and (2) tomographic optical imaging, increasing the resolution and physiological range using three different optical technologies: direct reflectance imaging, optical scanning microscopy, and diffuse optical tomography. These technologies will be validated against invasive "gold standard" techniques in studies of rat whisker barrel cortex and macaque visual cortex, and further applied to animal models spreading depression in migraine and stroke. Each of these experiments is designed to allow us to serially step from more to less invasive, and move from systems where much is already known through to studies in humans that have not heretofore been explored within the spatiotemporal domains our newly developed tools will afford.

描述（申请提供）：最后十年带来 革命性的新技术允许在工作中可视化工作大脑 人类在系统层面。但是，在 断层技术的时空分辨率（fMRI，PET）和 动物研究允许机械神经模型的电路水平。是 该提案的总体目标是开发一套综合技术 弥合了这个关键的差距。在整个过程中都发现了两个相互关联的主题 建议：（1）改善非侵入性的空间和时间分辨率 技术，它将可以直接对离散成像（E G列和 层流水平）神经单位，桥接系统和细胞水平，以及 （2）阐明与神经元活性生物物理学相关的机制 在我们的成像测量中，“可观察到”。这两个关键技术是 调查的是：（1）使用非常高的分辨率MRI和fMRI，使用非常高 强度梯度，分阶段阵列线圈以及3T和7T的其他进步 非人类灵长类动物，9.4T大鼠和（2）层析成像， 使用三种不同的光学来增加分辨率和生理范围 技术：直接反射成像，光学扫描显微镜和 弥漫性光学断层扫描。这些技术将被验证 大鼠晶须桶皮层研究中的侵入性“黄金标准”技术 猕猴的视觉皮层，并进一步应用于动物模型扩散 偏头痛和中风的抑郁症。这些实验中的每一个都设计为 允许我们从更多的侵入性逐步逐步发展，然后从系统中移动 在没有的研究中已经知道了很多 在我们新开发的时空域中探索了迄今探索 工具将负担得起。