Core--Essential Laboratory Services

核心——必要的实验室服务

• 批准号: 6758079
• 负责人: Judith Anne Berliner
• 金额: $ 29.73万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-08-01 至 2008-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6758079
• 关键词: atherosclerosis; biomedical facility; blood cells; blood chemistry; blood donor; blood lipoprotein; blood lipoprotein metabolism; clinical research; human subject; leukocytes; lipid metabolism; tissue /cell culture; atherosclerosis; biomedical facility; blood cells; blood chemistry; blood donor; blood lipoprotein; blood lipoprotein metabolism; clinical research; human subject; leukocytes; lipid metabolism; tissue /cell culture

The CHS portion of Core A provides human lipoproteins and leukocytes and general services to the Projects and other Cores. The MRL portion of Core A provides analytical chemistry assays for the Projects and other Cores. The CHS portion of Core A has provided essential laboratory services to our Program Project since its inception. All Projects and Cores have made extensive use of this Core. A major service provided by this Core is the preparation of blood products including human monocytes, lymphocytes and occassionally platelets as well as LDL, HDL and lipoprotein deficient serum (LPDS). Over the last year alone this Core isolated 10 billion monocytes, ten grams of lipoproteins, and 38 grams of LPDS. In addition, the Core provides standard assays, centralized glassware washing, autoclaving, maintenance of common facilities and radioisotope monitoring. This Core also isolates human aortic endothelial cells and smooth muscle cells and provides these as stock cultures to the Program's Investigators.

核心A的CHS部分为项目和其他核心提供了人类脂蛋白和白细胞以及一般服务。核心A的MRL部分为项目和其他核心提供了分析化学测定。核心A的CHS部分自成立以来为我们的计划项目提供了重要的实验室服务。所有项目和核心都广泛使用了该核心。该核心提供的主要服务是制备血液产品，包括人单核细胞，淋巴细胞和偶尔血小板以及LDL，HDL和脂蛋白缺乏血清（LPD）。仅在过去的一年中，这个核心就孤立了100亿个单核细胞，十克脂蛋白和38克LPD。此外，核心提供标准测定，集中式玻璃器皿洗涤，高压灭菌，公共设施的维护和放射性同位素监测。该核心还可以分离人类主动脉内皮细胞和平滑肌细胞，并将其作为该计划的研究人员提供库存培养物。