HIGH SPEED HIGH RESOLUTION PHASE RESOLVED OCT/ODT

高速高分辨率相位分辨 OCT/ODT

• 批准号: 6682871
• 负责人: ZHONGPING CHEN
• 金额: $ 26.33万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-01-18 至 2006-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6682871
• 关键词: animal tissue; bioimaging /biomedical imaging; biomedical equipment development; blood flow measurement; chick embryo; clinical research; heterodyning; human subject; image enhancement; image processing; laboratory mouse; light scattering; noninvasive diagnosis; optics; skin neoplasms; tomography

Direct visualization of tissue physiology and anatomy provides important information to the physician for the diagnosis and management of disease. High spatial resolution noninvasive techniques for imaging in vivo tissue structure and blood flow dynamics are currently not available as a diagnostic tool in clinical medicine. Such techniques could have a significant impact for biomedical research and patient treatment. The objective of the proposed research is to develop a high speed noninvasive optical technique, optical coherence tomography (OCT) and optical Doppler tomography (ODT), for imaging in vivo tissue structure and blood flow with high spatial resolution (2-10mum) in biological tissues. Preliminary results obtained in our laboratory have demonstrated the potential of this technology for a number of clinical applications where imaging tissue structure and monitoring hemodynamics are important. However, there are four limitations in our current OCT/ODT system: speed, resolution, penetration depth and speckle noise. The proposed research is directed toward the development of a high speed, high resolution, phase resolved OCT/ODT system for imaging tissue structure and microcirculation that overcomes these limitations. The specific aims of this proposal are to: (1) design and develop a high speed high resolution phase resolved OCT/ODT system for tomographic imaging of in vivo tissue structure and blood flow dynamics in highly scattering biological tissues; (2) develop signal processing and image reconstruction software and hardware for phase resolved OCT/ODT; (3) image blood flow in vitro using reconstituted canine blood and in vivo using the chick chorioallantoic membrane (CAM) model to verify and optimize OCT/ODT system operation and spatial resolution; and (4) demonstrate in animal models and clinical studies how OCT/ODT can assist in diagnosis and treatment of skin tumors and port wine stain birthmarks where imaging tissue structure and monitoring blood flow are important.

组织生理学和解剖学的直接可视化为医生诊断和治疗疾病提供了重要信息。 用于体内组织结构和血流动力学成像的高空间分辨率无创技术目前还不能用作临床医学的诊断工具。 此类技术可能对生物医学研究和患者治疗产生重大影响。 本研究的目的是开发一种高速无创光学技术、光学相干断层扫描（OCT）和光学多普勒断层扫描（ODT），用于在生物体内以高空间分辨率（2-10mum）对体内组织结构和血流进行成像。组织。 我们实验室获得的初步结果证明了该技术在许多临床应用中的潜力，在这些应用中，组织结构成像和血流动力学监测非常重要。 然而，我们当前的 OCT/ODT 系统存在四个限制：速度、分辨率、穿透深度和散斑噪声。 拟议的研究旨在开发一种高速、高分辨率、相分辨 OCT/ODT 系统，用于对组织结构和微循环进行成像，从而克服这些限制。该提案的具体目标是：（1）设计和开发高速高分辨率相分辨OCT/ODT系统，用于高散射生物组织中的体内组织结构和血流动力学的断层成像； (2) 开发用于相分辨OCT/ODT的信号处理和图像重建软硬件； (3) 使用重建的犬血液对体外血流进行成像，并使用鸡绒毛尿囊膜（CAM）模型对体内血流进行成像，以验证和优化 OCT/ODT 系统操作和空间分辨率； (4) 在动物模型和临床研究中证明 OCT/ODT 如何协助诊断和治疗皮肤肿瘤和鲜红斑痣，其中组织结构成像和血流监测非常重要。