Diamond and Hydrogenated Carbon Microelectrodes

金刚石和氢化碳微电极

• 批准号: 6631452
• 负责人: GREG M SWAIN
• 金额: $ 11.21万
• 依托单位: MICHIGAN STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-08-01 至 2005-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6631452
• 关键词: Raman spectrometry; X ray spectrometry; atomic force microscopy; bioassay; bioengineering /biomedical engineering; biomaterial development /preparation; biomaterial evaluation; biomaterial interface interaction; biotechnology; capillary electrophoresis; carbon; chemical kinetics; electrical measurement; electrochemistry; hydrogen; liquid chromatography; mass spectrometry; microelectrodes; neurotransmitters; oxidation reduction reaction; scanning electron microscopy; surface coating; surface property

DESCRIPTION (provided by applicant): The proposed research will fabricate, evaluate and develop diamond and hydrogenated carbon fiber microelectrodes for use in the measurement and detection of electroactive neurotransmitters, related metabolites and other bioanalytes. These new, low oxide and chemically stable microelectrodes have the potential to significantly impact in vitro and in vivo neuroelectrochemical measurements because they solve several problems/complications often encountered with common sp2 carbon fiber microelectrodes. These problems/complications exist because of the presence of electroactive and ionizable surface carbon-oxygen functionalities. Boron-doped microcrystalline and nitrogen-incorporated nanocrystalline diamond films conformally deposited on 20-100 micron diameter W and Mo fibers, and hydrogen plasma treated carbon fibers (pitch and pan-based, 10-40 micron diameter) will be the microelectrodes investigated. These electrode materials offer advantages compared to commonly used sp2 carbon electrodes including a lower voltammetric and amperometric background current; no background voltammetnc features associated with electroactive surface carbon-oxygen functionalities; no variations in the response sensitivity for charged solution analytes due to the absence of ionizable carbon-oxygen functionalities; resistance to fouling due to the nonpolar, hydrogen-terminated surface; low limits of detection; and improved response precision and stability.The goals of the research are (i) to prepare and comprehensively characterize the microelectrodes, (ii) to demonstrate their efficacy for the measurement of neurotransmitters, metabolites and bioanalytes, (iii) to verify that the low oxide surfaces solve the problems/complications that exist in fast scan voltammetric measurements when oxygen functionalized electrodes are used, (iv) to develop a detailed understanding of the electrode reaction kinetics and mechanisms at the hydrogen-terminated surfaces and (v) to apply the electrodes in FIA-EC, LC-EC and CE-EC assays with the objective of significantly improving the analytical detection figures of merit.

描述（由申请人提供）：拟议的研究将制造，评估和开发钻石和氢化的碳纤维微电极，以用于测量和检测电活性神经递质，相关代谢物和其他生物分析。这些新的，低氧化物和化学稳定的微电极具有显着影响体外和体内神经电性化学测量的潜力，因为它们解决了常见的SP2碳纤维微电极经常遇到的几个问题/并发症。这些问题/并发症的存在是由于存在电活性和可离子的表面碳氧功能。在20-100微米直径W和mo纤维上，掺杂硼掺杂的微晶和氮含量的纳米晶钻膜以及氢等离子体处理的碳纤维（螺距和锅基，基于底盘和锅基，10-40微米直径）将是微电极的研究。与常用的SP2碳电极相比，这些电极材料具有优势，包括降低伏安和安培背景电流。没有与电活性表面碳氧功能相关的伏安NC的特征；由于缺乏电离碳 - 氧功能，带电溶液分析物的响应灵敏度没有变化。由于非极性，氢终止的表面引起的污垢的抗性；较低的检测；并提高了响应精度和稳定性。研究的目标是（i）准备并全面地表征微电极，（ii）证明其对测量神经递质，代谢物和生物分析的测量的功效，以验证低氧氧化当使用氧官能化电极时，表面解决了快速扫描伏安测量测量中存在的问题/并发症，（iv）以详细了解电极反应动力学和氢终止表面和（v）的电极反应动力学和机制，以将电极施加FIA-EC，LC-EC和CE-EC分析的目的是显着改善优异的分析检测数字。