IMAGING VIRUS PARTICLES W/ 300 KEV ELECTRON CRYOMICROSCOPE

使用 300 KEV 电子低温显微镜对病毒颗粒进行成像

• 批准号: 6611296
• 负责人: Wah Chiu
• 金额: $ 13.47万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-12-01 至 2002-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6611296
• 关键词: biomedical resource; infection; technology /technique; virus

This project is aimed at evaluating the potential of a 300 keV electron cryomicroscope with a field emission gun and a liquid helium cryostage. Such an instrument is only available in Japan. We froze samples of P22 phage on EM grids in Houston and shipped them under liquid nitrogen to Dr. Y. Fujiyoshi s laboratory in Japan. Joanita Jakana and Wah Chiu performed the imaging in Japan. A few sets of quality images were obtained in a week of intensive work. Our subsequent computer analysis indicated that the damping of the computed transform of the images taken in Japan was not as steep as that of the 400 keV images taken with equivalent defocus in Houston. This preliminary data has provided the results needed to develop a grant proposal submitted to NSF for acquisition of such an instrument, which costs ~US$2 million, in the US. We have obtained a strong matching fund commitment from Baylor s administration to support this grant application. Notice of funding of $ 1 million from NSF has just been received today.

该项目旨在评估300 KEV的潜力 带有田间发射枪和液氦的电子冷冻显微镜 低温量。 这样的乐器仅在日本可用。 我们冻结 休斯顿的EM网格上的P22噬菌体样品并将其运送到下面 日本Y. Fujiyoshi的实验室的液氮。 Joanita Jakana和Wah Chiu在日本进行了成像。 几套 高质量的图像是在一周的大量工作中获得的。 我们的 随后的计算机分析表明 日本拍摄的图像的计算转换不像 休斯顿与同等散热器拍摄的400 keV图像中。 该初步数据提供了开发的结果 提交给NSF的赠款提案以获取此类工具， 在美国，这要花费约200万美元。 我们已经获得了强大的 匹配贝勒政府的基金承诺支持这一点 授予申请。 来自NSF的100万美元的资金通知仅 今天收到。