IMAGING USING HYPERPOLARIZED 3HE & ARTERIAL SPIN TAGGING MRI

使用超偏振 3HE 成像

• 批准号: 6657643
• 负责人: RAHIM R RIZI
• 金额: $ 22.55万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-01 至 2003-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6657643
• 关键词: Mammalia; animal tissue; bioimaging /biomedical imaging; biomedical resource; carbohydrates; diabetes mellitus; human tissue; magnetic resonance imaging; neoplasm /cancer; radiology; technology /technique; Mammalia; animal tissue; bioimaging /biomedical imaging; biomedical resource; carbohydrates; diabetes mellitus; human tissue; magnetic resonance imaging; neoplasm /cancer; radiology; technology /technique

Nuclear magnetic resonance spectroscopy (NMR) provides a method of noninvasively monitoring flux through the various pathways of energy metabolism. This is accomplished by examining the kinetics of 13C transfer from a suitable, 13C labeled substrate, and fitting data to an appropriate metabolic network model. In 1983, Chance et al. developed such a model for determining flux through the tricarboxylic acid (TCA) cycle of the heart from the kinetics of 13C glutamate labeling. We recently validated this model by comparing rates of oxygen comsumption by Langendorff perfused rat hearts with oxygen comsumption rates predicted from the model, glucose, acetate and b-hydroxybutyrate were substrates. The results were in excellent agreement after the original model was modified to include the malate-aspartate and glycerol-3-phosphate shuttles. We now propose to extend this model to incorporate other pathways of energy metabolism and to apply this model to other tissues such as tumo r, skeletal muscle, brain and liver.

核磁共振光谱（NMR）提供了一种方法 通过各种能量途径非侵入性监测通量 代谢。 这是通过检查13c的动力学来完成的 从合适的13C标记的底物转移，然后将数据拟合到 适当的代谢网络模型。 1983年，Chance等。 开发了这样的模型，用于确定通过三羧基的通量 13C谷氨酸动力学的心脏酸（TCA）循环 标签。 我们最近通过比较速率来验证了该模型 Langendorff的氧气消耗含氧 从模型，葡萄糖，乙酸盐和 B-羟基丁酸是底物。 结果表现出色 修改原始模型后的协议以包括 苹果酸 - 天冬氨酸和甘油-3-磷酸盐班车。 我们现在建议 扩展该模型以结合其他能量代谢途径 并将此模型应用于其他组织，例如tumo r，骨骼 肌肉，大脑和肝脏。