MODELING OF LASER INDUCED FLUORESCENCE SPECTROSCOPY IN TISSUE

组织中激光诱导荧光光谱的建模

• 批准号: 6611240
• 负责人: WARREN S GRUNDFEST
• 金额: $ 15.58万
• 依托单位: UNIVERSITY OF SOUTHERN CALIFORNIA
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-01 至 2003-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6611240
• 关键词: bioengineering /biomedical engineering; biomedical resource; communication behavior; nervous system; statistics /biometry; technology /technique

The nonlinear modeling methodology of "principal dynamic modes" (PDM) developed in Core Project #1, was applied to a spider mechanoreceptor. The fundamental question is the identification of the biophysical mechanisms that subserve mechanotransduction and the precise quantitative description of this process. The experiments (funded from other sources) employ broadband random stimuli and record either intracellularly or extracellularly. Results from the application of the PDM method to extracellular and intracellular recordings have shown excellent predictive ability of the resulting model, far exceeding the capabilities of previous models. Two PDM's have been identified in these preliminary studies, corresponding to two biophysical mechanisms: one that encodes intensity and another encoding rate of change. The latter PDM allows the mechanoreceptor to retain gain sensitivity at different operating points (adaptation). Comparisons between the results from intracellular and extracellular data were also made. This general approach can find application to other neural systems and, therefore, offers a powerful tool in a diverse and broad field of biological sciences.

“主动力模态”的非线性建模方法 (PDM) 在核心项目 #1 中开发，应用于蜘蛛 机械感受器。 根本问题是识别 促进机械转导的生物物理机制和 这个过程的精确定量描述。 实验 （由其他来源资助）采用宽带随机刺激并记录 无论是在细胞内还是在细胞外。 结果来自 PDM方法在细胞外和细胞内的应用 录音显示出对结果的出色预测能力 型号，远远超过以前型号的功能。 两个 PDM 在这些初步研究中已确定，对应于 两种生物物理机制：一种编码强度，另一种编码强度 编码变化率。 后者 PDM 允许机械感受器 保持不同工作点的增益灵敏度（适应）。 细胞内和细胞外结果的比较 还制作了数据。 这种通用方法可以应用于 其他神经系统，因此提供了一个强大的工具 生物科学的多样化和广泛领域。