CRYSTALLOGRAPHIC INVESTIGATIONS OF METAL ORGANIC NETWORK SOLIDS

金属有机网络固体的晶体学研究

• 批准号: 6667833
• 负责人: PETER T WOLCZANKI
• 金额: $ 14.27万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-30 至 2003-08-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6667833
• 关键词: bioimaging /biomedical imaging; biological products; biomedical resource; proteins; radiography; structural biology

Two projects involving structure determinations of proteins that are drug design targets are described. The proteins are the microsomal triglyceride transfer protein (MTP), and human cytomegalovirus (HCMV) protease. Each of these projects involves the use of three-dimensional structural information to aid in the design of new therapeutic agents. In each case, the goal is to inhibit a novel protein target. Each of these structures projects presents special challenges that require the use of a high brilliance synchrotron radiation source for data collection. In the case of MTP, limits of resolution of native and derivative data from crystals of this large protein (150 kDa) must be extended to solve the structure. In the case of HCMV protease, where two useful derivatives have been identified, limits of resolution, which are favorable along c*, must be extended in the rest of the sphere of reflection in order to produce an interpretable map.

两个涉及蛋白质结构确定的项目 描述了药物设计目标。 蛋白质是 微粒体甘油三酸酯转移蛋白（MTP）和人类 巨细胞病毒（HCMV）蛋白酶。 这些项目中的每一个都涉及 使用三维结构信息来帮助设计 新的治疗剂。 在每种情况下，目标是抑制 新型蛋白质靶标。 这些结构中的每一个项目都提出 需要使用高光彩的特殊挑战 数据收集的同步子辐射源。 在MTP的情况下 从晶体中分辨出天然和衍生数据的限制 必须扩展这种大蛋白（150 kDa）以解决结构。 在HCMV蛋白酶的情况下，有两个有用的衍生物是 确定的，分辨率的限制，沿C*有利，必须 在其余的反射范围内延伸，以便为了 产生一个可解释的地图。