NMR AND MR IMAGING STUDIES OF PLAQUE RUPTURE

斑块破裂的 NMR 和 MR 成像研究

• 批准号: 6642032
• 负责人: JAMES Athur HAMILTON
• 金额: $ 28.62万
• 依托单位: BOSTON UNIVERSITY MEDICAL CAMPUS
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-08-08 至 2006-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6642032
• 关键词: aorta; atherosclerosis; atherosclerotic plaque; bioimaging /biomedical imaging; biomarker; blood vessel occlusion; body temperature; calcium; chemical property; cholesterol; cholesterol esters; coronary occlusion /thrombosis; disease /disorder model; histochemistry /cytochemistry; laboratory rabbit; light microscopy; lipid structure; macrophage; magnetic resonance imaging; metalloendopeptidases; morphology; nuclear magnetic resonance spectroscopy; physical property; prognosis; thromboplastin

Although the advances have been made in the detection and treatment of vascular diseases, myocardial infarctions and strokes often strike apparently healthy persons without warning and produce disabilities or death. Atherosclerosis is the underlying cause of most heart attacks and strokes. Atherosclerotic plaques can grow slowly over time and gradually block blood flow, often producing symptoms that warn the patient of the underlying disease. However, less occlusive plaques can produce acute events within minutes by rupturing and abruptly forming an occlusive thrombus. These plaques appear to have certain physical characteristics, such as a thin fibrous cap and lipid-rich core, which distinguish them from less dangerous plaques. There is new urgency to evaluate vascular disease in humans by imaging methods that provide data about the ultrastructure of plaques, rather than invasive methods such as angiography that report only luminal narrowing. This project uses the modified Constantinides animal (rabbit) model of plaque rupture to compare plaque components and ultrastructure in non-ruptured and ruptured plaques. Magnetic resonance (MR) images of the aorta in rabbits (in vivo) will be obtained before and after triggering plaque rupture, and 9with higher resolution) after excision. Comparison of the MR images of ruptured and non-ruptured plaques will provide markers for plaque rupture and determine the value of MR imaging for predicting vulnerable plaques will provide markers for plaque rupture and determine the value of MR imaging for predicting vulnerable plaques in humans before rupture occurs. Magic angle spinning (MAS) NMR spectroscopy will be used to characterize in situ the composition of each lipid phase in excised plaques. MAS NMR allows quantitation of crystalline cholesterol, liquid and liquid-crystalline cholesteryl esters, and calcium salts in the intact plaque; each of these structures alone, or interactions between them, may play a role in plaque vulnerability. To enhance the interpretation of MR images, the detailed physical chemical information from MAS NMR will be integrated with the spatial information about lipid and protein components determined by magnetic resonance (MR) imaging and light microscopy/histology. Because the ultrastructure of plaques appears to be key to their stability and potential for regression, MR imaging has the potential for being a more reliable predictor of acute pathological events (heart attack and stroke).

尽管在检测和治疗方面取得了进展 血管疾病、心肌梗塞和中风经常发作 表面健康的人在没有任何警告的情况下造成残疾或死亡。 动脉粥样硬化是大多数心脏病发作和中风的根本原因。 动脉粥样硬化斑块会随着时间的推移缓慢生长并逐渐阻塞血液 流动，通常会产生警告患者潜在疾病的症状。 然而，较少闭塞的斑块可能会在几分钟内产生急性事件 破裂并突然形成闭塞性血栓。这些牌匾似乎 具有某些物理特征，例如薄的纤维帽和 富含脂质的核心，这将它们与危险性较低的斑块区分开来。有 通过成像方法评估人类血管疾病的新紧迫性 提供有关斑块超微结构的数据，而不是侵入性方法 例如仅报告管腔狭窄的血管造影。该项目使用 改良Constantinides动物（兔）斑块破裂模型进行比较 未破裂和破裂斑块中的斑块成分和超微结构。 兔子主动脉（体内）的磁共振 (MR) 图像将 在触发斑块破裂之前和之后获得的结果，以及 9 具有更高的 分辨率）切除后。破裂和破裂的 MR 图像比较 未破裂的斑块将为斑块破裂提供标记并确定 MR 成像在预测易损斑块方面的价值将为 斑块破裂并确定 MR 成像对预测易损性的价值 破裂发生之前人体中的斑块。魔角旋转 (MAS) NMR 光谱将用于原位表征每种脂质的组成 切除斑块的阶段。 MAS NMR 可以对晶体进行定量 胆固醇、液体和液晶胆固醇酯以及钙 完整斑块中的盐；这些结构中的每一个单独或相互作用 它们之间，可能在牙菌斑脆弱性中发挥作用。为了增强 MR 图像的解释，详细的物理化学信息 MAS NMR 将与脂质和蛋白质的空间信息集成 由磁共振 (MR) 成像和光确定的成分 显微镜/组织学。因为斑块的超微结构似乎是关键 由于其稳定性和回归潜力，MR 成像具有潜力 作为急性病理事件（心脏病发作）更可靠的预测因子 和中风）。