CORE--CELLULAR NEUROSCIENCE FACILITY

核心——细胞神经科学设施

• 批准号: 6652277
• 负责人: Gabriel Corfas
• 金额: $ 8.62万
• 依托单位: BOSTON CHILDREN'S HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-07-01 至 2003-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6652277
• 关键词: animal tissue; biomedical facility; confocal scanning microscopy; histopathology; human tissue; immunocytochemistry; in situ hybridization; mental retardation; neurosciences; tissue /cell preparation

DESCRIPTION: The Cellular Neuroscience Core was formed from the previous Histology and Electron Microscopy Cores. The Histology Core originated under the leadership of Dr. Richard Sidman, with support from the Chief Technologist, Mr. Pieter Dikkes. The Electron Microscopy Core was initiated under the leadership of Dr. Hannah Kinney, who was the first Director of the combined Cellular Neuroscience core. The current director, Dr. Gabriel Corfas, has de-emphasized the electron microscopy aspect of the service in favor of providing more extensive, high throughput services that better meet the current needs of the 37 investigators routinely using this Core. These methods of tissue preparation for structural and molecular anatomical examination include preparation of tissues for standard histology, electron microscopy, confocal microscopy, in situ hybridization, and immunocytochemistry. In addition, this Core provides perfusion of rodents for tissue examination. An emphasis of the Core is to train Center investigators and their associates in methods of tissue preparation. The Cellular Neuroscience Core also provides morphological and neuropathological consultation to investigators. Staff includes the Chief Technologist with 35 years of experience, and a to-be-named technologist with less experience.

描述：细胞神经科学核心是从上一个形成的 组织学和电子显微镜核心。组织学核心起源于 理查德·西曼（Richard Sidman）博士的领导，在酋长的支持下 技术专家Pieter Dikkes先生。启动电子显微镜核心 在汉娜·金尼（Hannah Kinney）博士的领导下，他是 组合细胞神经科学核心。 现任董事加布里埃尔·科法斯（Gabriel Corfas）博士已取消了电子 服务的显微镜方面有利于提供更广泛的高度 吞吐量服务可以更好地满足37名调查人员的当前需求 通常使用此核心。这些组织制备方法的结构方法 和分子解剖学检查包括准备组织 标准组织学，电子显微镜，共聚焦显微镜原位 杂交和免疫细胞化学。此外，此核心提供 啮齿动物进行组织检查。 核心的重点是培训中心调查员及其同事 在组织制备方法中。细胞神经科学核心 向研究人员提供形态学和神经病理学咨询。 员工包括拥有35年经验的首席技术人员， 具有较少经验的名称的技术专家。