Local Tissue Biomechanics in Low Back & Radicular Pain

腰部局部组织生物力学

• 批准号: 6660778
• 负责人: Beth A Winkelstein
• 金额: $ 12.22万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-19 至 2005-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6660778
• 关键词: RNase protection assay; back injury; backache; behavior test; biomechanics; chronic pain; enzyme linked immunosorbent assay; inflammation; laboratory rat; nerve injury; neural plasticity; neurochemistry; neuroimmunomodulation; pain threshold; spinal nerves; spine injury; statistics /biometry

DESCRIPTION (provided by the applicant): This application aims to test hypotheses regarding low back pain while also increasing the candidate?s skills in pain and neurophysiologic research. As an independent researcher, she proposes a plan that will help in the transition to an independent researcher, combining both focused research and career development activities. Her long-term career goal is the development of an independent research program studying painful neck injury biomechanics. Immediate goals for achieving this are provided in a career development plan for this award that include both courses related to neurobiology and research directly related to low back pain. Under the mentorship of Dr. DeLeo, she proposes to incorporate these activities in her training and research. Dartmouth is said to be an ideal environment for such activities due to Dr. DeLeo?s leadership in the pain field and the institution as a whole having much collaboration between Anesthesiology, the Spine Center, and the Thayer School of Engineering. The proposed research incorporates aspects of biomechanics and the neuroimnmunology of pain to test the central hypothesis that lumbar radioculopathy depends on local nerve root deformation and central neuroplasticity that is directly influenced by the initial tissue deformation magnitude. While mechanisms of low back and radicular pain are believed to involve a mechanical component, there is an incomplete understanding of the local mechanical response of neural tissue in these diseases. The proposed work combines studying neuroinflammatory mechanisms leading to spinal sensitization with bioengineering analysis of tissue injury. An existing rat model of lumbar radioculopathy is utilized to: (1) quantify and correlate differences in mechanical and spinal neuroimmune activation responses for non-inflammatory and inflammatory compressive insults, (2) quantitatively assess reproducibility in applying a nerve root injury, (3) determine local tissue mechanics and subsequent neuroinflammation associated with acute and chronic radicular pain states, and (4) use these data to develop both strain-based and load-based criteria for painful nerve root injury. To accomplish this, in vivo tissue strains will be correlated with mechanical allodynia and neuroimmune mediators of persistent pain.

描述（申请人提供）：此申请旨在测试 关于腰痛的假设，同时也提高了候选人技能 在疼痛和神经生理学研究中。作为一名独立研究人员，她 提出一项计划，该计划将有助于向独立研究人员过渡， 结合重点研究和职业发展活动。她 长期职业目标是制定独立研究计划 研究疼痛的颈部损伤生物力学。实现这一目标的直接目标 在该奖项的职业发展计划中提供，其中包括 与神经生物学和研究有关的课程与腰痛直接相关。 在Deleo博士的指导下，她建议将这些活动纳入 在她的培训和研究中。据说达特茅斯是理想的环境 由于Deleo博士在疼痛领域的领导和 整体机构在麻醉学之间进行了很多合作， 脊柱中心和Thayer工程学院。拟议的研究 结合生物力学的各个方面和疼痛的神经肌无力进行测试 腰部辐射病的中心假设取决于局部神经根 变形和中央神经塑性直接影响 初始组织变形幅度。而腰背机制和 据信根部疼痛涉及机械成分，有一个 对神经组织的局部机械反应不完全理解 这些疾病。拟议的工作结合了研究神经炎症性 通过生物工程分析的机制导致脊柱敏化 组织损伤。现有的腰椎放射性肿瘤模型用于： （1）量化和相关的机械和脊髓神经免疫性的差异 非炎症和炎症压缩侮辱的激活反应， （2）定量评估施加神经根损伤时的可重复性，（3） 确定局部组织力学和随后的神经炎症相关 使用急性和慢性辐射疼痛状态，（4）使用这些数据来发展 基于应变和基于负荷的疼痛神经根损伤的标准。到 这样做，体内组织菌株将与机械相关 持续性疼痛的异常性痛和神经免疫介质。