Local Tissue Biomechanics in Low Back & Radicular Pain

腰部局部组织生物力学

• 批准号: 6660778
• 负责人: Beth A Winkelstein
• 金额: $ 12.22万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-19 至 2005-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6660778
• 关键词: RNase protection assay; back injury; backache; behavior test; biomechanics; chronic pain; enzyme linked immunosorbent assay; inflammation; laboratory rat; nerve injury; neural plasticity; neurochemistry; neuroimmunomodulation; pain threshold; spinal nerves; spine injury; statistics /biometry

DESCRIPTION (provided by the applicant): This application aims to test hypotheses regarding low back pain while also increasing the candidate?s skills in pain and neurophysiologic research. As an independent researcher, she proposes a plan that will help in the transition to an independent researcher, combining both focused research and career development activities. Her long-term career goal is the development of an independent research program studying painful neck injury biomechanics. Immediate goals for achieving this are provided in a career development plan for this award that include both courses related to neurobiology and research directly related to low back pain. Under the mentorship of Dr. DeLeo, she proposes to incorporate these activities in her training and research. Dartmouth is said to be an ideal environment for such activities due to Dr. DeLeo?s leadership in the pain field and the institution as a whole having much collaboration between Anesthesiology, the Spine Center, and the Thayer School of Engineering. The proposed research incorporates aspects of biomechanics and the neuroimnmunology of pain to test the central hypothesis that lumbar radioculopathy depends on local nerve root deformation and central neuroplasticity that is directly influenced by the initial tissue deformation magnitude. While mechanisms of low back and radicular pain are believed to involve a mechanical component, there is an incomplete understanding of the local mechanical response of neural tissue in these diseases. The proposed work combines studying neuroinflammatory mechanisms leading to spinal sensitization with bioengineering analysis of tissue injury. An existing rat model of lumbar radioculopathy is utilized to: (1) quantify and correlate differences in mechanical and spinal neuroimmune activation responses for non-inflammatory and inflammatory compressive insults, (2) quantitatively assess reproducibility in applying a nerve root injury, (3) determine local tissue mechanics and subsequent neuroinflammation associated with acute and chronic radicular pain states, and (4) use these data to develop both strain-based and load-based criteria for painful nerve root injury. To accomplish this, in vivo tissue strains will be correlated with mechanical allodynia and neuroimmune mediators of persistent pain.

描述（由申请人提供）：本申请旨在测试 关于腰痛的假设，同时也提高了候选人的技能 从事疼痛和神经生理学研究。作为一名独立研究员，她 提出了一项有助于向独立研究员过渡的计划， 将重点研究和职业发展活动结合起来。她 长期职业目标是开发独立的研究计划 研究疼痛性颈部损伤生物力学。实现这一目标的近期目标 该奖项的职业发展计划中提供了以下内容： 与神经生物学相关的课程以及与腰痛直接相关的研究。 在 DeLeo 博士的指导下，她建议将这些活动纳入其中 在她的训练和研究中。达特茅斯据说是一个理想的环境 这些活动归功于 DeLeo 博士在疼痛领域的领导地位以及 整个机构在麻醉学、 脊柱中心和塞耶工程学院。拟议的研究 结合了疼痛的生物力学和神经免疫学方面进行测试 腰椎放射性病变依赖于局部神经根的中心假设 变形和中枢神经可塑性直接受 初始组织变形幅度。虽然腰背和 根性疼痛被认为涉及机械成分，有一个 对神经组织局部机械反应的不完全了解 这些疾病。拟议的工作结合了神经炎症研究 通过生物工程分析导致脊柱过敏的机制 组织损伤。现有的腰椎放射性病变大鼠模型用于： (1) 量化并关联机械和脊髓神经免疫的差异 非炎症和炎症压迫损伤的激活反应， (2) 定量评估施加神经根损伤的再现性，(3) 确定局部组织力学和随后相关的神经炎症 急性和慢性根性疼痛状态，以及（4）使用这些数据来制定 基于应变和基于负荷的疼痛性神经根损伤标准。到 为了实现这一点，体内组织应变将与机械力相关 持续性疼痛的异常性疼痛和神经免疫介质。