DVLP WWW BASED NEURAL NETWORK ANALYZER FOR GXM DATABASE: CRYPTOCOCCUS, AIDS

用于 GXM 数据库的基于 DVLP WWW 的神经网络分析仪：隐球菌、艾滋病

• 批准号: 6493645
• 负责人: FARAMARZ VALAFAR
• 金额: $ 28.8万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-08-01 至 2002-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6493645
• 关键词: biological products; biomedical resource; carbohydrates; spectrometry; technology /technique

Fourier transform-infrared (FT-IR) spectroscopy is being developed as a stand-alone method for glycosyl composition analysis and as an adjunct to capillary electrophoretic (CE) separation of the monosaccharide components of glycoconjugates. Early results indicated that FT-IR spectroscopy can be used to differentiate among a wide spectrum of monosaccharide isomers, and that buffers typically used for CE separations do not interfere with IR detection. More recently, a new experimental method has been developed for FT-IR quantitative analysis with the use of FT-IR microscopy. Film spectra of sample solutions deposited and dried on a ZnSe plate were collected with an FT-IR microscope and a high-sensitivity mercury cadmium telluride detector. A fairly extensive library of monosaccharide IR spectra has been collected at high resolution under different conditions. The library is being extended to include di- and higher oligosaccharides. Recent work has focused on the pote ntial of performing glycosyl composition analysis by FT-IR spectroscopy of per-N,O-acetylated derivatives of the monosaccharide methylglycoside mixtures obtained after acidic methanolysis of mono- and oligosaccharides. The peracetylation step was introduced after methanolysis because the monosaccharide methylglycosides were observed to have poor deposition characteristics. Since succesful application of the method will depend on prior acquisition of training spectra from a wide distribution of known samples, including pure monosaccharides as well as standard mixtures, a large number of these samples were generated from combinations of sugars known to comprise N- and O-linked oligosaccharides of mammalian glycoproteins. Spectra from 145 standards of known composition have been acquired, and the method is now being tested on unknown oligosaccharides and monosaccharide mixtures.

傅里叶变换红外 (FT-IR) 光谱学正在开发中 作为糖基组成分析的独立方法和 毛细管电泳 (CE) 分离的辅助手段 复合糖的单糖成分。 早期结果表明 FT-IR 光谱可用于区分广泛的 单糖异构体谱以及通常使用的缓冲液 CE 分离不会干扰 IR 检测。 最近， 开发了一种新的 FT-IR 定量实验方法 使用 FT-IR 显微镜进行分析。 样品的薄膜光谱 用收集器收集沉积在 ZnSe 板上并干燥的溶液 FT-IR 显微镜和高灵敏度碲化汞镉 探测器。 一个相当广泛的单糖红外光谱库 在不同条件下以高分辨率采集。 这 文库正在扩展以包括二糖和高级寡糖。 最近的工作重点是进行糖基化的潜力 通过 FT-IR 光谱法对全 N,O-乙酰化物进行成分分析 获得的单糖甲基糖苷混合物的衍生物 单糖和寡糖的酸性甲醇分解后。 这 全乙酰化步骤是在甲醇解之后引入的，因为 观察到单糖甲基糖苷沉积不良 特征。 由于该方法的成功应用将 取决于事先从广泛的训练光谱中获取 已知样品的分布，包括纯单糖 作为标准混合物，生成了大量这些样品 来自已知包含 N- 和 O- 连接的糖的组合 哺乳动物糖蛋白的寡糖。 145 的光谱 已获得已知成分的标准，方法是 现在正在对未知的寡糖和单糖进行测试 混合物。