PLASTICITY OF NEURONAL FUNCTION AND FORM

神经元功能和形式的可塑性

• 批准号: 6477324
• 负责人: CHUN-FANG WU
• 金额: $ 21.16万
• 依托单位: UNIVERSITY OF IOWA
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-07-01 至 2003-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6477324
• 关键词: Drosophilidae; animal genetic material tag; behavioral /social science research tag; calmodulin dependent protein kinase; cell differentiation; cyclic AMP; developmental genetics; developmental neurobiology; embryogenesis; enzyme activity; gene mutation; invertebrate embryology; larva; neural plasticity; neural transmission; neurogenesis; neurogenetics; potassium channel; synapses; tissue /cell culture

DESCRIPTION: Neurogenetic studies in Drosophila have contributed significantly to our understanding of behavioral plasticity and the underlying molecular mechanisms. This project is a continuation of our long-term efforts to bridge the gap between these two levels of approach by elucidating the associated modifications in neuronal function and structure and in neural circuit performance. A combination of genetic, molecular, morphological and physiological techniques will be employed to analyze a collection of mutants selected for distinct behavioral alterations with identified molecular defects. Previous studies from this lab have demonstrated altered neuronal firing patterns and synaptic plasticity, as well as the abnormal habituation process of an escape circuit, in the memory mutants dnc and rut with defective cAMP metabolism and in the K+channel mutants Hk and eag. This grant proposes to continue to investigate the roles of second messenger cascades at the neuronal and circuit levels, extending from the cAMP cascade (dnc and rut) to mutations that affect CaM kinase (ala), and cGMP-dependent kinase (for). Like dnc and rut, ala mutants show learning disabilities, while for exhibits altered foraging behavior. Double mutants have been constructed for analyzing effects derived from convergence and cross talk between different signal transduction pathways. In addition, K+ channels control neuronal firing properties and are known as the targets of modulation by second messenger cascades. Mutations of different K+channel subunits, Sh and slo, as well as Hk and eag, will be examined in parallel to determine how they affect synaptic plasticity and circuit performance. This study will include the habituation, a non-associative conditioning process, in the adult escape circuit, and the plasticity of synaptic efficacy and terminal sprouting in the larval neuromuscular junctions. Analysis of firing properties and synaptic function in central neurons will be facilitated by the giant neuron culture system.

描述：果蝇的神经遗传学研究做出了贡献 对我们对行为可塑性的理解和 潜在的分子机制。 这个项目是我们的延续 通过长期努力来弥合这两个层面的方法之间的差距 阐明神经元功能和结构的相关修饰 以及神经回路的性能。 遗传、分子、 将采用形态学和生理学技术来分析 选择具有不同行为改变的突变体集合 识别出分子缺陷。 该实验室之前的研究表明 表现出改变的神经元放电模式和突触可塑性， 以及记忆中逃逸回路的异常习惯过程 突变体 dnc 和 rut 具有 cAMP 代谢缺陷和 K+通道缺陷 突变体 Hk 和 eag。 这笔赠款建议继续调查 第二信使级联在神经元和回路水平上的作用， 从 cAMP 级联（dnc 和 rut）延伸到影响 CaM 的突变 激酶 (ala) 和 cGMP 依赖性激酶 (for)。 就像 dnc 和 rut 一样，阿拉 突变体表现出学习障碍，而对于展品则改变了觅食方式 行为。 构建双突变体用于分析效果 来自不同信号之间的收敛和串扰 转导途径。 此外，K+通道控制神经元放电 属性并被称为第二信使调制的目标 级联。 不同 K+ 通道亚基 Sh 和 slo 的突变，以及 HK 和 eag 将同时进行检查以确定它们如何影响 突触可塑性和电路性能。 这项研究将包括 习惯化，一种非联想调节过程，在成人逃避中 电路，以及突触功效和终端萌芽的可塑性 幼虫的神经肌肉接头。 烧成特性分析 巨神经元将促进中枢神经元的突触功能 文化体系。