Multiresolution Autofocusing for Automated Cytogenetics

用于自动细胞遗传学的多分辨率自动聚焦

• 批准号: 6443502
• 负责人: QIANG WU
• 金额: $ 9.17万
• 依托单位: ADVANCED DIGITAL IMAGING RESEARCH, LLC
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-05-15 至 2003-04-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6443502
• 关键词: artificial intelligence; bioimaging /biomedical imaging; biomedical automation; clinical research; computer program /software; computer system design /evaluation; cytogenetics; digital imaging; fluorescence microscopy; fluorescent in situ hybridization; human data; image enhancement; image processing; mathematical model

The goal of this project is to develop innovative digital microscope autofocusing techniques for automated cytogenetics applications. We propose a novel multi-resolution image analysis approach to focus measurement and detection, based on the recently developed mathematical theory of wavelet transform. In comparison to currently available single-resolution techniques, the proposed method overcomes their fundamental limitations and promises considerably more accurate, reliable and faster means to compute and determine in-focus image position for image acquisition. This will significantly increase the ability and efficacy of automated scanning microscope instruments for clinical and cancer cytogenetics applications. In Phase 1 we will investigate the feasibility of the proposed method based on its utilization in fluorescence microscopy. We will develop and implement the algorithm and software for multi-resolution focus function computation and in-focus position determination. We will test and evaluate the new method against the current best-performing algorithms by comparing (1) Accuracy; (2) Range; (3) Insensitivity to other parameters; and (4) Speed. If the new approach achieves superior performance, in Phase 2 the technique will be further developed and extended to bright-field microscopy applications. When fully developed, the new technology will be made available to Applied Imaging (AIC) for integration into the PowerGene cytogenetics automation products. PROPOSED COMMERCIAL APPLICATIONS: As soon as the new techniques are developed and qualified for routine application, they will be made available to AIC for incorporation into the PowerGene product line of cytogenetics automation equipment, both in new systems sold and as an upgrade to existing systems already in use in cytogenetics labs, thus commercializing the technology quickly.

该项目的目标是开发用于自动化细胞遗传学应用的创新数字显微镜自动对焦技术。 我们基于最近发展的小波变换数学理论，提出了一种新颖的多分辨率图像分析方法来进行焦点测量和检测。 与当前可用的单分辨率技术相比，所提出的方法克服了它们的基本局限性，并有望以更加准确、可靠和更快的方式来计算和确定图像采集的聚焦图像位置。 这将显着提高自动扫描显微镜仪器在临床和癌症细胞遗传学应用中的能力和功效。在第一阶段，我们将根据所提出的方法在荧光显微镜中的应用来研究其可行性。 我们将开发并实现用于多分辨率聚焦函数计算和对焦位置确定的算法和软件。 我们将通过比较（1）准确性；与当前性能最佳的算法来测试和评估新方法； (2) 范围； (3) 对其他参数不敏感； (4)速度。如果新方法实现卓越的性能，在第二阶段，该技术将得到进一步开发并扩展到明场显微镜应用。 开发完成后，新技术将可供 Applied Imaging (AIC) 集成到 PowerGene 细胞遗传学自动化产品中。拟议的商业应用：一旦新技术开发出来并符合常规应用资格，它们将提供给 AIC，用于将其纳入 PowerGene 细胞遗传学自动化设备产品线，无论是在销售的新系统中还是作为现有系统的升级在细胞遗传学实验室中使用，从而使该技术快速商业化。