CORE--ELECTRON MICROSCOPY

核心--电子显微镜

• 批准号: 6302766
• 负责人: HENRY J RALSTON
• 金额: $ 18.85万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-01-15 至 2000-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6302766
• 关键词: bioimaging /biomedical imaging; biomedical facility; confocal scanning microscopy; electron microscopy; immunofluorescence technique; nociceptors; tissue /cell preparation

Core A is an essential component of this Program Project, as it serves the research needs of four of the five Projects proposed by the Principal and Co-Investigators. The major research instrument in the Core is a JEOL 100 CX II electron microscope, originally purchased by funds primarily provided by this Program Project in 1986. The electron microscope permits analysis of neurons and their synaptic connections. Many of the studies proposed in this application deal with changes in neural networks that are believed to underlie pain syndromes, and the work could not be carried out without the instrument. The funds requested in the Core A budget support part of the salary of a senior electron microscopy technician, who oversees the unit, trains new users of the microscope, and keeps it in optimal running order. Funds are also requested to pay 50% of the annual cost of a service contract. An additional instrument, a BioRad Scanning Confocal microscope, is housed in the Keck Foundation Center for Integrative Neuroscience; three of the Co-Investigators are members of the Center. The confocal microscope is used to examine populations of neurons stained by immunofluorescence to identify various synaptic transmitters and receptors. Several of the research projects in this proposal make use of this instrument as a research tool. Fifty percent of the annual cost of a service contract of the confocal microscope is requested in this proposal.

核心 A 是该计划项目的重要组成部分，因为它满足了首席研究员和联合研究员提出的五个项目中四个项目的研究需求。核心中的主要研究仪器是 JEOL 100 CX II 电子显微镜，最初由该计划项目于 1986 年提供的资金购买。电子显微镜可以分析神经元及其突触连接。本申请中提出的许多研究涉及神经网络的变化，这些变化被认为是疼痛综合征的基础，如果没有仪器，这项工作就无法进行。核心 A 预算中要求的资金支持高级电子显微镜技术员工资的一部分，该技术员负责监督该装置、培训显微镜的新用户并使其保持最佳运行状态。还要求资金支付服务合同年度费用的 50%。另外一台仪器是 BioRad 扫描共聚焦显微镜，位于凯克基金会综合神经科学中心；三名联合调查员是该中心的成员。共聚焦显微镜用于检查免疫荧光染色的神经元群，以识别各种突触递质和受体。本提案中的几个研究项目利用该仪器作为研究工具。该提案要求共焦显微镜服务合同年度费用的百分之五十。