CALCIUM REGULATION OF SECRETION IN NEUROENDOCRINE CELLS

神经内分泌细胞钙分泌的调节

• 批准号: 6177062
• 负责人: THOMAS F. J. MARTIN
• 金额: $ 21.18万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-06-10 至 2002-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6177062
• 关键词: PC12 cells; binding proteins; calcium ion; cell membrane; intermolecular interaction; membrane fusion; microinjections; molecular cloning; neuroendocrine system; phosphatidylinositols; protein structure function; restriction mapping; secretion; site directed mutagenesis; western blottings; PC12 cells; binding proteins; calcium ion; cell membrane; intermolecular interaction; membrane fusion; microinjections; molecular cloning; neuroendocrine system; phosphatidylinositols; protein structure function; restriction mapping; secretion; site directed mutagenesis; western blottings

DESCRIPTION: Based on preliminary results the P.I. has proposed an interesting and reasonable central hypothesis, that CAPS acts on membranes to facilitate membrane interactions or fusion by interacting with PIP2. The P.I. proposes to test this hypothesis by addressing four specific aims. The goal of specific aim 1 is to characterize the mechanism of CAPS action. The goal of specific aim 2 is to begin to analyze the structure of CAPS and to investigate conformational changes in CAPS upon binding to PIP2. The goal of specific aim 3 is to characterize the CAPS null phenotype in neural cells. The goal of specific aim 4 is to characterize a new cytosolic factor that may be involved in triggering fusion.

描述：基于初步结果P.I.提出了一个 有趣且合理的中心假设，帽子作用于膜 通过与PIP2相互作用来促进膜相互作用或融合。 P.I.提议通过解决四个特定的特定来检验这一假设 目标。特定目标1的目的是表征帽的机理 行动。特定目标2的目的是开始分析 盖子并研究瓶盖的构象变化在结合到与 PIP2。特定目标3的目的是表征盖子无效 神经细胞中的表型。特定目标4的目标是表征 可能涉及触发融合的新的胞质因子。