PROTEIN METABOLISM IN CHRONIC RENAL FAILURE PATIENTS

慢性肾衰竭患者的蛋白质代谢

• 批准号: 6336812
• 负责人: VICTORIA S LIM
• 金额: $ 0.6万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-08-01 至 2001-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6336812
• 关键词: Mammalia; aging; biological products; biomedical resource; cardiovascular system; musculoskeletal system; proteins; spectrometry

To study protein metabolism in patients with end-stage renal disease, we measured leucine kinetics in 8 patients. Study was performed twice before and once after (period III)( initiation of maintenance dialysis treatment. Before dialysis, one measurement was done when the patients are acidotic (period I), and the other during sodium bicarbonate supplementation when acidosis was corrected (period II). Sequence of Periods I and II were randomized. Subjects were on identical constant diet during all three periods. Leucine kinetics was measured during primed-constant infusion of [1-13C]leucine, [1-13C]KIC or ( keto-isocaproate and expired breath CO2 enrichment were directly determined. These values were used to calculate leucine appearance rates into the plasma leucine pool and into the total body leucine pool and leucine oxidation rate. The former represents protein degradation rat; protein synthesis rate was indirectly estimated by mass balance equation. The h igher leuc ine oxidation rate without bicarbonate supplementation was not due to higher VCO2 as total CO2 production rate was not different during the three periods. Alternatively, higher proportion of endogenously produced CO2 may be excreted than in the non-acidotic state. Finally, these findings may represent a real enhancement of amino acid oxidation in the presence of metabolic acidosis.

终末期肾病患者蛋白质代谢研究 疾病，我们测量了 8 名患者的亮氨酸动力学。 研究是 在（第三期）之前和之后执行两次（启动 维持性透析治疗。 透析前，一项测量是 当患者出现酸中毒时（第一期）进行，另一次则在 纠正酸中毒后补充碳酸氢钠（期间 二）。 第一期和第二期的顺序是随机的。 主题为 在所有三个时期都保持相同的恒定饮食。 亮氨酸动力学 在[1-13C]亮氨酸的引物持续输注期间测量， [1-13C]KIC 或（酮异己酸酯和呼出气体 CO2 富集 被直接确定。 这些值用于计算亮氨酸 血浆亮氨酸池和全身的出现率 亮氨酸库和亮氨酸氧化率。 前者代表 蛋白质降解大鼠；蛋白质合成率间接 通过质量平衡方程估计。 较高的亮氨酸氧化 没有补充碳酸氢盐的情况下的比率并不是由于 VCO2 较高，因为 三个时期的总二氧化碳产生率没有差异。 或者，更高比例的内源产生的二氧化碳可能是 排泄量高于非酸中毒状态。 最后，这些发现可能 代表存在下氨基酸氧化的真正增强 代谢性酸中毒。