PROTEIN METABOLISM IN CHRONIC RENAL FAILURE PATIENTS

慢性肾衰竭患者的蛋白质代谢

• 批准号: 6336812
• 负责人: VICTORIA S LIM
• 金额: $ 0.6万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-08-01 至 2001-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6336812
• 关键词: Mammalia; aging; biological products; biomedical resource; cardiovascular system; musculoskeletal system; proteins; spectrometry

To study protein metabolism in patients with end-stage renal disease, we measured leucine kinetics in 8 patients. Study was performed twice before and once after (period III)( initiation of maintenance dialysis treatment. Before dialysis, one measurement was done when the patients are acidotic (period I), and the other during sodium bicarbonate supplementation when acidosis was corrected (period II). Sequence of Periods I and II were randomized. Subjects were on identical constant diet during all three periods. Leucine kinetics was measured during primed-constant infusion of [1-13C]leucine, [1-13C]KIC or ( keto-isocaproate and expired breath CO2 enrichment were directly determined. These values were used to calculate leucine appearance rates into the plasma leucine pool and into the total body leucine pool and leucine oxidation rate. The former represents protein degradation rat; protein synthesis rate was indirectly estimated by mass balance equation. The h igher leuc ine oxidation rate without bicarbonate supplementation was not due to higher VCO2 as total CO2 production rate was not different during the three periods. Alternatively, higher proportion of endogenously produced CO2 may be excreted than in the non-acidotic state. Finally, these findings may represent a real enhancement of amino acid oxidation in the presence of metabolic acidosis.

研究末期肾脏患者的蛋白质代谢 疾病，我们测量了8例患者的亮氨酸动力学。 研究是 在（周期III）之前和一次执行两次（启动 维持透析治疗。 透析之前，一个测量是 当患者酸性（i时），另一个在 校正酸中毒时补充碳酸氢钠（周期 ii）。 时期I和II的顺序是随机的。 受试者启动 在所有三个时期，恒定饮食相同。 亮氨酸动力学 在[1-13c]亮氨酸的启动恒定输注期间测量 [1-13C] KIC或（酮置甲甲酸酯和过期的呼吸二氧化碳富集 直接确定。 这些值用于计算亮氨酸 血浆亮氨酸池的外观速率并进入总体 亮氨酸池和亮氨酸氧化速率。 前者代表 蛋白质降解大鼠；蛋白质合成速率间接 通过质量平衡方程估计。 较高的Leuc ine氧化 没有补充碳酸氢盐的速率并不是由于VCO2较高而引起的 在这三个时期，二氧化碳的总生产率没有差异。 或者，内源产生的CO2的比例较高。 排出比非酸化状态。 最后，这些发现可能 代表在存在下的真正增强氨基酸氧化 代谢性酸中毒。