ANTIGEN I/II INTERACTIONS WITH SALIVARY GLYCOPROTEINS

抗原 I/II 与唾液糖蛋白的相互作用

基本信息

批准号：
6379855
负责人：
DONALD R DEMUTH
金额：
$ 23.25万
依托单位：
UNIVERSITY OF PENNSYLVANIA
依托单位国家：
美国
项目类别：
财政年份：
1998
资助国家：
美国
起止时间：
1998-09-30 至 2003-07-31
项目状态：
已结题

项目摘要

Streptococci are important integral components of the microbial biofilm that is present on the surface of oral tissues and are intimately involved in the dynamic processes that govern the establishment, growth and control of this biofilm. Their interactions with salivary proteins are important for initiating bacterial colonization of oral tissues and their subsequent interactions with other oral microbes contribute to the maturation of plaque. Many of these reactions are mediated by a family of related streptococcal proteins collectively known as the antigen I/II polypeptides. However, the mechanisms defining antigen I/II interactions with human and bacterial components in the oral cavity and their relationships and contributions to oral diseases are not fully understood. The goals of this proposal are to understand how the function of antigen I/II proteins differ among species of oral streptococci, how the structure of the antigen I/II polypeptide influences its function, and how the structure and activities of antigen I/II proteins are affected by the oral environment. To accomplish these goals, the Principal Investigator will determine if the expression of multiple antigen I/II proteins by streptococci results in a tighter association of these organisms with oral tissues. He will correlate specific structural characteristics of different antigen I/II proteins with their functions. In addition, studies are proposed to determine if antigen I/II functions as a dimer and if the environment within the oral cavity affects antigen I/II function by influencing the stability of the dimer interface. An understanding of how antigen I/II contributes to the initiation and growth of the oral microbial biofilm will lead to a better understanding of how pathogenic organisms become established in the oral cavity and how minor structural differences that occur within related groups of proteins can dramatically influence their function. Finally, understanding the higher order structure of the antigen I/II proteins may lead to the development of broad spectrum anti-microbial agents that function by disrupting specific protein- protein interactions.

链球菌是微生物生物膜的重要积分成分它存在于口腔组织的表面，并且是紧密的参与管理建立，增长的动态过程并控制该生物膜。它们与唾液蛋白的相互作用对于启动口腔组织的细菌定殖和他们随后与其他口腔微生物的相互作用有助于斑块的成熟。这些反应中的许多是由家庭介导的相关的链球菌蛋白统称为抗原I/II 多肽。但是，定义抗原I/II的机制与口腔中的人类和细菌成分相互作用，他们对口腔疾病的关系和贡献尚未完全理解。该提议的目标是了解抗原I/II蛋白质的功能在口服的物种中不同链球菌，抗原I/II多肽的结构如何影响其功能以及抗原的结构和活动如何 I/II蛋白质受口腔环境的影响。完成这些目标，首席调查员将确定是否表达链球菌的多种抗原I/II蛋白会导致更紧密这些生物与口腔组织的关联。他会关联不同抗原I/II蛋白的特定结构特征与他们的功能。此外，提出了研究以确定如果抗原I/II用作二聚体，以及口腔通过影响稳定性影响抗原I/II功能二聚体接口的。对抗原I/II的理解有助于口服微生物生物膜的启动和生长将使人们更好地了解病原体如何变为在口腔中建立，以及如何较小的结构差异发生在相关的蛋白质组中可以极大地影响其功能。最后，了解抗原I/II蛋白可能导致广谱的发展通过破坏特定蛋白质的作用的抗微生物剂蛋白质相互作用。