Unravelling the physiological drivers of species accumulation and sensitivity for metals

揭示物种积累和金属敏感性的生理驱动因素

基本信息

  • 批准号:
    NE/W006200/1
  • 负责人:
  • 金额:
    $ 92.53万
  • 依托单位:
  • 依托单位国家:
    英国
  • 项目类别:
    Research Grant
  • 财政年份:
    2022
  • 资助国家:
    英国
  • 起止时间:
    2022 至 无数据
  • 项目状态:
    未结题

项目摘要

Metals are one of the most common pollutants of our soils. However, whether these potential poisons are taken up by an organism and what the subsequent toxic effects are is only known for a small number of species - usually those easily maintained in the laboratory or very common and widespread in the field. The limited scope of our current understanding of metal "accumulation" and the linked "toxicity" in different species makes it difficult for us to predict and monitor the negative effects that metal pollution has on ecosystems - whether directly due to toxicity or as a result of predators eating contaminated prey. We know from past work that even among the limited species tested, metal accumulation and toxicity can vary greatly. Further, we already know some of the main ways that metals can cause toxicity and also some of the systems active in cells by which such effects can be prevented through "detoxification". For metals this process is often associated with changing the form of the metal to an inert inorganic compound, locking it away into intracellular compartments or binding it to different proteins and/or peptides. What we currently do not know, however, is how these mechanisms and systems contrast between species and how this inter-species variations, in turn, lead to differences in the extent of accumulation and linked toxic effect. In this project, we want to develop a "framework" that unifies understanding of how metals are taken up by different soil animals, distributed between tissues, change their chemical associations and cause damage to cells, organs and the organism as a whole, resulting ultimately in toxicity for a species. Our framework is based on developing understanding in three areas.i) Measuring the rates at which a metal enters into, and is distribute, between the tissue of soil animals. We will quantify this by analysing metal levels in major tissues, using radio-labelled compounds to assess uptake and loss and generating tissue maps to determine where and how much metal is accumulating in the body. For larger invertebrates we will dissect the tissue but for the smaller organisms we will use state-of-the-art laser assisted mapping technologies. ii) Assessing the way that metals change their chemical associations on up taken into an organism, either through chemical reactions, compartmentalisation or by binding. We will measure these processes by analysing the chemical form of the metals within different pools in separated tissue samples and by using X-ray methods that determine the co-localisation of metals with other elements and known metal binding molecules. iii) Evaluating the resultant damage to cells and tissues from exposure to the different metal forms.This will be measured by assessing biochemical responses associated with damage and linking these to the pathways that regulate metal accumulation and the chemical form present within the organism. We will determine how the damage caused translates through various levels of biological organisation to result in toxicity at the level of the whole organism.Studying these three aspects for four metals (manganese, lead, copper and cadmium) that have different dominant chemistries and essentialities in eight common and ecologically important soil invertebrate species, will allow us to develop a new model that describes the processes that lead to metal accumulation and toxicity. This approach will greatly improve on the current approaches used in ecosystem focused toxicology, which have so far focused on external metal chemistry in the soil and how this impacts on exposure. Further, developing this organism-focused model, will allow us to extend our studies to other species beyond those studied here to more easily predict how much of a given metal each may accumulate and just what toxic impacts will result. This capacity will significantly advance on the current approaches used in comparative ecotoxicology.
金属是我们土壤中最常见的污染物之一。然而,这些潜在的毒物是否被生物体吸收以及随后的毒性作用是什么,只有少数物种知道——通常是那些在实验室中容易维持的物种,或者在野外非常常见和广泛存在的物种。我们目前对不同物种中金属“积累”和相关“毒性”的理解范围有限,这使得我们很难预测和监测金属污染对生态系统的负面影响 - 无论是直接由于毒性还是由于捕食者吃受污染的猎物。我们从过去的工作中知道,即使在测试的有限物种中,金属积累和毒性也可能有很大差异。此外,我们已经知道金属引起毒性的一些主要方式,以及细胞中一些活跃的系统,通过“解毒”可以防止这种影响。对于金属来说,这个过程通常与将金属的形式改变为惰性无机化合物、将其锁定在细胞内区室或将其与不同的蛋白质和/或肽结合有关。然而,我们目前不知道的是,这些机制和系统如何在物种之间形成对比,以及这种物种间差异如何反过来导致积累程度和相关毒性效应的差异。在这个项目中,我们希望开发一个“框架”,统一理解金属如何被不同的土壤动物吸收、分布在组织之间、改变它们的化学关联并对细胞、器官和整个生物体造成损害,最终导致对一个物种的毒性。我们的框架基于对三个领域的理解。i) 测量金属进入土壤动物组织并在土壤动物组织之间分布的速率。我们将通过分析主要组织中的金属水平来量化这一点,使用放射性标记的化合物来评估吸收和损失,并生成组织图来确定金属在体内积累的位置和数量。对于较大的无脊椎动物,我们将解剖其组织,但对于较小的生物体,我们将使用最先进的激光辅助绘图技术。 ii) 评估金属在被生物体吸收后改变其化学关联的方式,无论是通过化学反应、区室化还是通过结合。我们将通过分析分离的组织样本中不同池中金属的化学形态,并使用 X 射线方法确定金属与其他元素和已知金属结合分子的共定位来测量这些过程。 iii) 评估因暴露于不同金属形式而对细胞和组织造成的损害。这将通过评估与损害相关的生化反应并将这些反应与调节金属积累和生物体内存在的化学形式的途径联系起来来测量。我们将确定所造成的损害如何通过不同层次的生物组织转化,从而在整个生物体水平上产生毒性。研究四种金属(锰、铅、铜和镉)的这三个方面,它们在化学中具有不同的主要化学性质和本质。八种常见且对生态重要的土壤无脊椎动物物种将使我们能够开发一个新模型来描述导致金属积累和毒性的过程。这种方法将极大地改进目前以生态系统为重点的毒理学方法,这些方法迄今为止主要关注土壤中的外部金属化学及其对暴露的影响。此外,开发这种以生物体为中心的模型将使我们能够将我们的研究扩展到此处研究之外的其他物种,以便更容易地预测每种特定金属可能积累多少以及将产生什么毒性影响。这种能力将显着推进当前比较生态毒理学中使用的方法。

项目成果

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David Spurgeon其他文献

US doctors sue over “unfair” scheme for finding jobs for medical graduates
美国医生起诉医学毕业生寻找工作的“不公平”计划
  • DOI:
  • 发表时间:
    2002
  • 期刊:
  • 影响因子:
    0
  • 作者:
    David Spurgeon
  • 通讯作者:
    David Spurgeon
Canada reports more than 300 suspected cases of SARS
加拿大报告300多例SARS疑似病例
  • DOI:
    10.1136/bmj.326.7395.897/a
  • 发表时间:
    2003-04-26
  • 期刊:
  • 影响因子:
    0
  • 作者:
    David Spurgeon
  • 通讯作者:
    David Spurgeon
Outpatient thyroidectomy is safe in selected patients
门诊甲状腺切除术对于特定患者是安全的
  • DOI:
  • 发表时间:
    2006
  • 期刊:
  • 影响因子:
    0
  • 作者:
    David Spurgeon
  • 通讯作者:
    David Spurgeon
Canadians launch class action over E coli outbreak
加拿大人就大肠杆菌爆发发起集体诉讼
  • DOI:
    10.1136/bmj.321.7252.11/b
  • 发表时间:
    2000-07-01
  • 期刊:
  • 影响因子:
    0
  • 作者:
    David Spurgeon
  • 通讯作者:
    David Spurgeon
Strategies for robust and accurate experimental approaches to quantify nanomaterial bioaccumulation across a broad range of organisms
  • DOI:
    10.1039/c8en01378k
  • 发表时间:
    2019-04
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Elijah J. Petersen;Monika Mortimer;Robert M. Burgess;Richard Handy;Shannon Hanna;Kay T. Ho;Monique Johnson;Susana Loureiro;Henriette Selck;Janeck J. Scott-Fordsmand;David Spurgeon;Jason Unrine;Nico W. van den Brink;Ying Wang;Jason White;Patricia Holden
  • 通讯作者:
    Patricia Holden

David Spurgeon的其他文献

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{{ truncateString('David Spurgeon', 18)}}的其他基金

Simply forever: Tackling PFAS complexity through mode of action assignment
永远简单:通过行动模式分配解决 PFAS 复杂性
  • 批准号:
    NE/Z000084/1
  • 财政年份:
    2024
  • 资助金额:
    $ 92.53万
  • 项目类别:
    Research Grant
Classic and temporal mixture synergism in terrestrial ecosystems: Prevalence, mechanisms and impacts
陆地生态系统中的经典和时间混合协同作用:普遍性、机制和影响
  • 批准号:
    NE/S000224/2
  • 财政年份:
    2019
  • 资助金额:
    $ 92.53万
  • 项目类别:
    Research Grant
Classic and temporal mixture synergism in terrestrial ecosystems: Prevalence, mechanisms and impacts
陆地生态系统中的经典和时间混合协同作用:普遍性、机制和影响
  • 批准号:
    NE/S000224/1
  • 财政年份:
    2018
  • 资助金额:
    $ 92.53万
  • 项目类别:
    Research Grant
Tracking relevant nanomaterial transformations, exposure, uptake and effects in freshwater and soil systems
跟踪淡水和土壤系统中相关纳米材料的转化、暴露、吸收和影响
  • 批准号:
    NE/N006224/1
  • 财政年份:
    2015
  • 资助金额:
    $ 92.53万
  • 项目类别:
    Research Grant
Leveraging comparative physiology and genomics to predict species sensitivity: A novel framework for interspecies extrapolation in ecotoxicology
利用比较生理学和基因组学来预测物种敏感性:生态毒理学中种间外推的新框架
  • 批准号:
    NE/M01438X/1
  • 财政年份:
    2015
  • 资助金额:
    $ 92.53万
  • 项目类别:
    Research Grant
Developing biomarkers to characterise the impact of emerging environmental pollutants in freshwater Chironomids (Insecta, Diptera)
开发生物标志物来表征新兴环境污染物对淡水摇蚊(昆虫纲、双翅目)的影响
  • 批准号:
    NE/N00065X/1
  • 财政年份:
    2015
  • 资助金额:
    $ 92.53万
  • 项目类别:
    Research Grant
Distinguishing pollutant-induced stresses from spatial and temporal environmental heterogeneity - a metabolomic approach to stress ecology
区分污染物引起的应激与时空环境异质性——应激生态学的代谢组学方法
  • 批准号:
    NE/H005382/1
  • 财政年份:
    2010
  • 资助金额:
    $ 92.53万
  • 项目类别:
    Research Grant
Modelling and measurement of Cd exposure and pathology in human volunteers living in proximity to a smelter source
对居住在冶炼厂附近的人类志愿者的镉暴露和病理学进行建模和测量
  • 批准号:
    NE/E008585/1
  • 财政年份:
    2008
  • 资助金额:
    $ 92.53万
  • 项目类别:
    Research Grant
Modelling and measurement of Cd exposure and pathology in human volunteers living in proximity to a smelter source
对居住在冶炼厂附近的人类志愿者的镉暴露和病理学进行建模和测量
  • 批准号:
    NE/E00895X/1
  • 财政年份:
    2007
  • 资助金额:
    $ 92.53万
  • 项目类别:
    Research Grant

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Unravelling the cellular mechanisms underpinning within- and trans-generational physiological and life history responses of marine invertebrates exposed to multiple global change drivers using a multi-layer approach
使用多层方法揭示暴露于多种全球变化驱动因素的海洋无脊椎动物的代内和跨代生理和生活史反应的细胞机制
  • 批准号:
    RGPIN-2020-05627
  • 财政年份:
    2022
  • 资助金额:
    $ 92.53万
  • 项目类别:
    Discovery Grants Program - Individual
Unravelling the cellular mechanisms underpinning within- and trans-generational physiological and life history responses of marine invertebrates exposed to multiple global change drivers using a multi-layer approach
使用多层方法揭示暴露于多种全球变化驱动因素的海洋无脊椎动物的代内和跨代生理和生活史反应的细胞机制
  • 批准号:
    RGPIN-2020-05627
  • 财政年份:
    2022
  • 资助金额:
    $ 92.53万
  • 项目类别:
    Discovery Grants Program - Individual
Unravelling the cellular mechanisms underpinning within- and trans-generational physiological and life history responses of marine invertebrates exposed to multiple global change drivers using a multi-layer approach
使用多层方法揭示暴露于多种全球变化驱动因素的海洋无脊椎动物的代内和跨代生理和生活史反应的细胞机制
  • 批准号:
    RGPIN-2020-05627
  • 财政年份:
    2021
  • 资助金额:
    $ 92.53万
  • 项目类别:
    Discovery Grants Program - Individual
Unravelling the cellular mechanisms underpinning within- and trans-generational physiological and life history responses of marine invertebrates exposed to multiple global change drivers using a multi-layer approach
使用多层方法揭示暴露于多种全球变化驱动因素的海洋无脊椎动物的代内和跨代生理和生活史反应的细胞机制
  • 批准号:
    RGPIN-2020-05627
  • 财政年份:
    2021
  • 资助金额:
    $ 92.53万
  • 项目类别:
    Discovery Grants Program - Individual
Unravelling the cellular mechanisms underpinning within- and trans-generational physiological and life history responses of marine invertebrates exposed to multiple global change drivers using a multi-layer approach
使用多层方法揭示暴露于多种全球变化驱动因素的海洋无脊椎动物的代内和跨代生理和生活史反应的细胞机制
  • 批准号:
    RGPIN-2020-05627
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    2020
  • 资助金额:
    $ 92.53万
  • 项目类别:
    Discovery Grants Program - Individual
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