QUANTITATIVE MICROTOMOGRAPHY OF BONE TRABECULAE

骨小梁的定量显微成像

• 批准号: 6399095
• 负责人: Michael J Flynn
• 金额: $ 28.05万
• 依托单位: HENRY FORD HEALTH SYSTEM
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-09-25 至 2004-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6399095
• 关键词: X ray; bioengineering /biomedical engineering; bioimaging /biomedical imaging; biomedical equipment development; bone imaging /visualization /scanning; bone regeneration; computed axial tomography; dogs; electromagnetic radiation; genetically modified animals; hormone therapy; laboratory mouse; method development; normal ossification; osteocytes; osteogenesis imperfecta; osteoporosis; ovariectomy; postmenopause; somatotropin; substantia spongiosa; synchrotrons; tomography

DESCRIPTION (provided by applicant): X-ray computed tomography of the spine and hip is an established method for quantitative bone mineral measurement that resolves elements with 1-millimeter size. For bone specimens, high-resolution laboratory computed tomography systems can describe cancellous bone architecture with resolved elements of about 50 micrometers. This is sufficient to describe bone volume, trabecular thickness and orientation, and connectivity. We have recently developed a new innovative method to perform quantitative computed tomography on an individual bone trabecula with resolved elements of 1 micrometer. Whole trabeculae are separated from cancellous bone specimens and scanned in a 250 micrometer diameter saline filled tube. A beam of x-rays from a synchrotron source is focused using a zone plate and used to make numerous measurements of radiation transmission. The measured data is then reconstructed to a 600 x 600 x 540 array of values having a spacing of 1/3 micrometers in the X, Y, and Z direction. High speed scanning devices will be used on this project to enable examination of 16 specimens per day. This method should be extremely valuable for evaluating the microscopic properties of bone tissue with respect to the mineralization of the matrix and porosity associated with osteocyte lacunae. To establish this value, four biomedical experiments are proposed for which genetic, pharmacologic, and biomechanic variables will be used to alter the bone tissue in animals. Prior experience indicates that these models should alter the mineralization and porosity at a microscopic scale.

描述（由申请人提供）：脊柱的X射线计算机断层扫描和 髋关节是一种定量骨矿物质测量的既定方法 解决具有1毫米大小的元素。对于骨标本，高分辨率 实验室计算机断层扫描系统可以描述取消骨头 结构已解决约50微米的元素。这足够了 描述骨骼体积，小梁厚度和方向，以及 连接性。我们最近开发了一种新的创新方法来执行 分辨的单个骨头小梁上的定量计算机断层扫描 1微米的元素。 整个小梁与取消骨标本分开，并在A中进行扫描 250微米直径盐水填充管。一束来自同步器的X射线 源使用区域板进行聚焦，并用于进行大量测量 辐射传输。然后将测量数据重建为600 x 600 x 540值阵列在x，y和z中具有1/3微米的间距 方向。该项目将使用高速扫描设备来启用 每天检查16个标本。 该方法对于评估微观应该非常有价值 骨组织的特性相对于基质的矿化和 孔隙率与骨细胞间隙相关。为了建立这个价值，四个 提出了生物医学实验的遗传，药理学和 生物力学变量将用于改变动物的骨组织。事先的 经验表明，这些模型应改变矿化化和 微观尺度上的孔隙率。