SOL XRAY SCATTER STUDY OF BACTERIOPHAGE P22 CAPSID & ITS SCAFFOLDING PROTEIN

噬菌体 P22 衣壳的 SOL X 射线散射研究

• 批准号: 2788409
• 金额: $ 0.37万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2788409
• 关键词: biomaterials; biomedical resource; proteins

We continued solution scattering measurements of the wild-type and mutant P22 capsid. Instrument calibration was done more carefully this time than last year, and we observed scattering peaks whose Bragg spacing match very well with those calculated from the EM structures that the Baylor group had obtained. In the higher angle region, we have a few characteristic peaks that may arise from b-sheet structure of the capsid protein and/or from RNA packing inside. We measured solution x-ray scattering from the scaffolding protein in the temperature range 15 - 60 !C. Clear changes in the assembly state of the scaffolding protein were seen in subsidiary scattering peaks. The studies were continued on a separate experimental proposal by W. Chiu et al.

我们继续解决野生型和的散射测量 突变体P22衣壳。 仪器校准进行了更仔细地进行 这次比去年，我们观察到散射峰的bragg 间距与从EM结构计算的那些非常匹配 贝勒集团获得了。 在更高角度的区域中，我们 有一些特征峰可能是由B片结构引起的 内部的capsid蛋白和/或从RNA堆积中。 我们测量了 溶液X射线散射从脚手架蛋白 温度范围15-60！c。 大会状态的明显变化 在子公司散射峰中可见脚手架蛋白。 这 W. chiu对单独的实验建议进行了研究 等。