PROGRAMS FOR ACQUISITION & ANALYSIS OF 4D MICROSCOPY DATA SETS

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• 批准号: 2757603
• 金额: $ 0.05万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2757603
• 关键词: Invertebrata; animal tissue; biomedical resource; computers; growth factor; human tissue; microscopy; parasitism; vaccines

The goal of this project is to develop a suit of software programs that will nllow for the capture and analysis of multiple focal-plane, time-lapse movies. The acquisition of 3-dimensional time-lapse (4D) data sets involves the precise orchestration of a variety of hardware components including z-focus control motors, illumination shutters, digitizing video framegrabbers, serial I/O ports, etc. (Thomas et al. (1996) Science, 273, 603-607, Thomas dc White (1996) Sci. Comp. World, 24, 31-32). In the past, the IMR's 4D Live Cell Microscopy program has relied on an elaborate set of control macros for the freeware image processing application "NlH-Image" to perform 4D data acquisition. While it deDmitely provided a usable system, the problem with this strategy was that NIH-Image macros are limited to 32 kb in size. The IMR's 4D acquisition macros quickly reached, and then exceeded this limit. Adding new features to the software or supporting new hardware components meant removing existing features or support. The goal of this subproject was to develop a non-dependent stand-alone Macintosh application for the acquisition of 4D data sets which would be unlimited in the amount of features that could be added and the number of hardware components which could be supported. It was also hoped that by optimizing the software specifically for 4D acquisition, that improvements in acquisition speed and ease-of-use would also be realized. The result of this project, the "4D Grabber" application, is a significant step forward in 4D acquisition technology. Not only does the "4D Grabber" application seamlessly coordinate the various hardware components of a 4D Live Cell microscopy workstation, but does so using easy to-understand graphic user interfaces in order to reduce the learning curve for the researcher wishing to perform these types of experiments in their own labs. The software supports 6 common digitizing framegrabber cards of both the Nubus and PCI slot configuration (including the ability to take advantage of on-card frame averaging, if present), and supports four different stage drive motors. Its modular code design makes adding support for additional hardware systems very straightforward. Significant increases in collection speed were also realized with the software's optimized design. An additional advantage of the "4D Grabber" software is the ability to gather 4D data directly to compressed QuickTime movies. By taking advantage of this feature, it is possible to gather up to 15 times as many data points per given hard disk space than when using previous 4D collection systems. This collection mode also offers a very impressive savings in time since the lengthy data translation stage (which was formerly necessitated with all 4D data sets) can be completely eliminated. This application, like all the others in the IMR's 4D Imaging Suite, will be available free-of-charge to researchers all over the world via downloading from the Internet.

该项目的目标是开发一套软件程序 这将允许捕获和分析多个焦平面， 延时电影。 3维延时（4D）的采集 数据集涉及各种硬件的精确编排 组件包括 z 焦点控制电机、照明百叶窗、 数字化视频图像采集卡、串行 I/O 端口等（Thomas et al. (1996) 科学，273, 603-607，托马斯 dc 怀特 (1996) 科学。比较。 世界，24、31-32）。 过去，IMR 的 4D 活细胞显微镜 程序依赖于一组精心设计的控制宏 用于执行 4D 数据的免费图像处理应用程序“NlH-Image” 获得。 虽然它确实提供了一个可用的系统，但问题是 采用这种策略的原因是 NIH-Image 宏的大小被限制为 32 kb 尺寸。 IMR的4D采集宏很快就达到了，然后 超过了这个限制。 向软件添加新功能或 支持新的硬件组件意味着删除现有功能或 支持。 该子项目的目标是开发一个非依赖的 用于采集 4D 数据集的独立 Macintosh 应用程序 可以添加的功能数量是无限的 以及可以支持的硬件组件的数量。 它 还希望通过专门针对 4D 的软件进行优化 采集，提高采集速度和易用性 也将得以实现。 该项目的成果“4D Grabber” 应用程序，是 4D 采集领域向前迈出的重要一步 技术。 “4D Grabber”应用不仅无缝 协调 4D Live Cell 的各种硬件组件 显微镜工作站，但使用易于理解的图形来实现 用户界面，以减少学习曲线 希望自己进行此类实验的研究人员 实验室。 该软件支持6种常见的数字化图像采集卡 Nubus 和 PCI 插槽配置（包括能够 利用卡上帧平均（如果存在），并支持 四个不同的阶段驱动电机。 其模块化的代码设计使得 添加对其他硬件系统的支持非常简单。 收集速度也显着提高 软件的优化设计。 “4D”的另一个优点 Grabber”软件能够直接收集 4D 数据 压缩的 QuickTime 电影。 通过利用这一特性， 每个给定可以收集多达 15 倍的数据点 硬盘空间比使用以前的 4D 采集系统时要少。 这 收集模式还节省了非常可观的时间，因为 冗长的数据转换阶段（以前需要 与所有 4D 数据集）可以完全消除。 这 与 IMR 4D 成像套件中的所有其他应用程序一样，该应用程序将 通过以下方式免费向世界各地的研究人员提供 从互联网上下载。