PROGRAMS FOR ACQUISITION & ANALYSIS OF 4D MICROSCOPY DATA SETS

收购计划

• 批准号: 2757603
• 金额: $ 0.05万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2757603
• 关键词: Invertebrata; animal tissue; biomedical resource; computers; growth factor; human tissue; microscopy; parasitism; vaccines

The goal of this project is to develop a suit of software programs that will nllow for the capture and analysis of multiple focal-plane, time-lapse movies. The acquisition of 3-dimensional time-lapse (4D) data sets involves the precise orchestration of a variety of hardware components including z-focus control motors, illumination shutters, digitizing video framegrabbers, serial I/O ports, etc. (Thomas et al. (1996) Science, 273, 603-607, Thomas dc White (1996) Sci. Comp. World, 24, 31-32). In the past, the IMR's 4D Live Cell Microscopy program has relied on an elaborate set of control macros for the freeware image processing application "NlH-Image" to perform 4D data acquisition. While it deDmitely provided a usable system, the problem with this strategy was that NIH-Image macros are limited to 32 kb in size. The IMR's 4D acquisition macros quickly reached, and then exceeded this limit. Adding new features to the software or supporting new hardware components meant removing existing features or support. The goal of this subproject was to develop a non-dependent stand-alone Macintosh application for the acquisition of 4D data sets which would be unlimited in the amount of features that could be added and the number of hardware components which could be supported. It was also hoped that by optimizing the software specifically for 4D acquisition, that improvements in acquisition speed and ease-of-use would also be realized. The result of this project, the "4D Grabber" application, is a significant step forward in 4D acquisition technology. Not only does the "4D Grabber" application seamlessly coordinate the various hardware components of a 4D Live Cell microscopy workstation, but does so using easy to-understand graphic user interfaces in order to reduce the learning curve for the researcher wishing to perform these types of experiments in their own labs. The software supports 6 common digitizing framegrabber cards of both the Nubus and PCI slot configuration (including the ability to take advantage of on-card frame averaging, if present), and supports four different stage drive motors. Its modular code design makes adding support for additional hardware systems very straightforward. Significant increases in collection speed were also realized with the software's optimized design. An additional advantage of the "4D Grabber" software is the ability to gather 4D data directly to compressed QuickTime movies. By taking advantage of this feature, it is possible to gather up to 15 times as many data points per given hard disk space than when using previous 4D collection systems. This collection mode also offers a very impressive savings in time since the lengthy data translation stage (which was formerly necessitated with all 4D data sets) can be completely eliminated. This application, like all the others in the IMR's 4D Imaging Suite, will be available free-of-charge to researchers all over the world via downloading from the Internet.

该项目的目的是制定软件程序的诉讼 对于捕获和分析多个焦点平面的捕获和分析， 延时电影。 获得3维延时（4D） 数据集涉及各种硬件的精确编排 包括Z对焦电机，照明百叶窗，包括的组件， 数字化视频框架，串行I/O端口等（Thomas等人。 （1996）Science，273，603-607，Thomas DC White（1996）Sci。 comp。 世界，24，31-32）。 过去，IMR的4D活细胞显微镜 程序依赖于一套精心的控制宏 免费软件图像处理应用程序“ NLH-图像”以执行4D数据 获得。 虽然它专门提供了一个可用的系统，但问题 有了这种策略，NIH-图像宏限制为32 kb 尺寸。 IMR的4D收购宏很快到达，然后 超出了此限制。 向软件添加新功能或 支持新的硬件组件意味着删除现有功能或 支持。 该子项目的目的是发展非依赖性 独立的Macintosh应用程序以获取4D数据集 这将是可以添加的功能的数量无限的 以及可以支持的硬件组件的数量。 它 还希望通过专门为4D优化软件 获取，提高采集速度和易用性 也将实现。 该项目的结果是“ 4D抓手” 应用是4D收购的重要一步 技术。 不仅“ 4D Grabber”应用程序无缝 协调4D活细胞的各种硬件组件 显微镜工作站，但使用易于理解的图形进行 用户界面以减少学习曲线 希望自己执行这些类型的实验的研究人员 实验室。 该软件支持6张常见的数字化FrameGrabber卡 Nubus和PCI插槽配置（包括 如果存在的话，可以利用平均框架平均）并支持 四个不同的舞台驱动器。 它的模块化代码设计使 非常简单地增加对其他硬件系统的支持。 收集速度的显着提高也通过 软件的优化设计。 “ 4D”的另一个优势 grabber“软件是能够将4D数据直接收集到 压缩Quicktime电影。 通过利用此功能， 可以收集到每次给出的数据点的15倍 硬盘空间比使用以前的4D收集系统时。 这 收集模式也可以及时提供非常令人印象深刻的节省 冗长的数据翻译阶段（以前是必要的 使用所有4D数据集）可以完全消除。 这 像IMR 4D成像套件中的所有其他应用一样，应用程序也将 通过向世界各地的研究人员免费提供 从互联网下载。