Workshop for 'omics methodology development: use of secretome enriched meta-transcriptome sequencing for understanding interactions in diseased corals

“组学方法开发研讨会：利用分泌蛋白组富集的元转录组测序来了解患病珊瑚的相互作用”

基本信息

批准号：
NE/S013415/1
负责人：
Thomas Richards
金额：
$ 5.87万
依托单位：
UNIVERSITY OF EXETER
依托单位国家：
英国
项目类别：
Research Grant
财政年份：
2019
资助国家：
英国
起止时间：
2019 至无数据
项目状态：
已结题

来源：
https://gtr.ukri.org/projects?ref=NE%2FS013415%2F1
关键词：
Workshop omics methodology development use

项目摘要

Coral reefs are beautiful, unique and important ecosystems. Coral ecosystems are home to a huge range of biodiversity, conduct important ecosystem services and support many tropical fisheries and their associated human societies.Coral reefs are dying at an unprecedented rate leading to catastrophic ecosystem collapse, which is threatening biodiversity conservation and reducing the sustainability of many tropical fisheries around the globe. Climate change, over-fishing and pollution are recognised as the major contributing factors to coral reef decline. Furthermore, the significant increase in coral infectious disease load and diversity are also recognised as critical threats to coral reef ecosystems. In many cases climate change, over-fishing and pollution are recognised as the drivers of increased coral disease load and diversity.Corals rely on complex interactions with the microbes that live on and inside their bodies. The nature of these interactions, both beneficial and negative, are therefore dependant on numerous environmental factors and ecological interactions. These interactions are incredibly difficult to study in natural coral reef ecosystems and very difficult to reconstitute accurately in laboratory experiments. As such we drastically need new methods that make use of cutting edge large-scale DNA sequencing platforms so we can study these interactions directly in natural environments. Many of the complex interactions that corals undergo, with the microbes that live on and inside their bodies, are mediated through genes that encode proteins that exit out of the cell into the external environment. These proteins are functional in the external environment, interacting with other microbes and the host coral cells. In some cases, these proteins function to cause disease, for example by killing or perturbing the coral cells. The aim of this project is to bring together an international community of scientists to develop a new methodology for studying how microbes interact with coral (and vice versa) through specifically sampling and sequencing the genes that encode the specialised proteins that exit the cell and function in the external environment. By developing this protocol, we will have a new powerful and direct method that will enable us to study how microbes interact with coral to cause disease. Having established this method we will build public access resources and detailed protocols so that other scientists can then use this methodological approach to study a range of coral reef diseases and further adapt the approach for the study of additional infectious disease interactions in natural environments. This is important because it will unlock the door to studying a whole range of complex interactions in new ways directly from the environment, interactions which cannot be realistically recapitulated in a laboratory setting.

珊瑚礁是美丽，独特和重要的生态系统。珊瑚生态系统是众多生物多样性的所在地，进行重要的生态系统服务并支持许多热带渔业及其相关的人类社会。珊瑚礁正以前所未有的速度垂死，导致灾难性的生态系统崩溃，这是威胁生物多样性保护的威胁性生物多样性保护和降低许多Trop fermecer of tropical fisheries the tropical fishere is的可持续性。气候变化，过度捕获和污染被认为是珊瑚礁下降的主要因素。此外，珊瑚传染病负荷和多样性的显着增加也被认为是对珊瑚礁生态系统的关键威胁。在许多情况下，气候变化，过度捕获和污染被认为是珊瑚疾病负荷增加和多样性的驱动因素。核心依赖于与生活在其体内和体内的微生物的复杂相互作用。因此，这些相互作用的性质，无论是有益还是负面，都取决于许多环境因素和生态相互作用。这些相互作用在自然的珊瑚礁生态系统中很难研究，并且在实验室实验中很难准确重建。因此，我们急需使用新的方法来利用尖端的大规模DNA测序平台，以便我们可以直接在自然环境中研究这些相互作用。珊瑚经历的许多复杂相互作用，与生活在其体内和体内的微生物的许多复杂相互作用都是通过编码从细胞出口进入外部环境的蛋白质的基因介导的。这些蛋白质在外部环境中起作用，与其他微生物和宿主珊瑚细胞相互作用。在某些情况下，这些蛋白质起作用会引起疾病，例如通过杀死或扰动珊瑚细胞。该项目的目的是汇集一个国际科学家社区，以开发一种新方法，用于研究微生物如何通过专门采样和测序编码在外部环境中解释细胞和功能的专门蛋白质的基因，以研究微生物如何与珊瑚（反之亦然）相互作用。通过制定该协议，我们将拥有一种新的强大而直接的方法，使我们能够研究微生物如何与珊瑚相互作用以引起疾病。建立了这种方法后，我们将建立公共访问资源和详细的方案，以便其他科学家可以使用这种方法学方法来研究一系列珊瑚礁疾病，并进一步适应自然环境中其他传染病相互作用的方法。这很重要，因为它将直接从环境中以新的方式研究整个复杂相互作用的大门，这些相互作用在实验室环境中无法现实地概括。