SCOR IN SUDDEN CARDIAC DEATH

心源性猝死中的 SCOR

• 批准号: 2029131
• 负责人: Jay W. Mason
• 金额: $ 131.38万
• 依托单位: UNIVERSITY OF UTAH
• 依托单位国家: 美国
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-02-01 至 1999-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2029131
• 关键词: arrhythmia; electrophysiology; sudden cardiac death

We propose a Specialized Center of Research in Sudden Death at the University of Utah to investigate the relationship between abnormalities of repolarization and arrhythmic death. The investigative group is composed of 17 investigators and 8 consultants. Four Research Projects and four Core support laboratories are proposed. The first research project, lonic Mechanisms of Repolarization in Human Ventricular Myocytes, directed by MC Sanguinetti, centers on K+ and other repolarizing currents. Voltage clamp techniques are applied to isolated human myocytes to determine which currents are present, to fully characterize them and to determine how they influence normal and abnormal repolarization processes. In Project 2, Molecular and Physiologic Mechanisms of the Long QT Syndrome, MT Keating and colleagues investigate the molecular genetics and the cardiac electrophysiology of this inherited form of arrhythmia in man and in human myocardium. JW Mason and coworkers plan studies of repolarization changes that occur immediately preceding sudden death due to ischemia in the third project, Repolarization Changes Associated with Ventricular Arrhythmias and Sudden Death. In Project 4, Electrocardiographic Measures of Ventricular Repolarization, techniques for non-invasive measurement of regional cardiac repolarization and disparity of repolarization are developed by LS Green, RL Lux and colleagues for diagnosis and prognostication in man. The Administrative Core, led by Mason, will provide assistance to all investigators in budget management and intercommunication. Core 2, the Cell Processing Laboratory directed by WH Barry and GA Peeters, will prepare isolated human ventricular myocytes for Projects 1 and 2, maintain and culture frog oocytes for Project 1, and transform and maintain peripheral blood lymphocytes from patients with LQT for DNA analysis in Project 2. Core 3, the Repolarization Signal Processing Laboratory led by Lux, will provide electrocardiographic signal processing and interpretation capabilities to Projects 2, 3 and 4. TE Moon directs the fourth Core laboratory, Clinical Data Management and Biostatistics, which will assist Project 2, 3 and 4 investigators in collection, statistical analysis and interpretation of clinical data obtained from patients. This SCOR proposal is sharply focussed on a single facet of the problem of sudden death. It combines basic science with whole-organ physiology and complex clinical medicine. It is likely to produce insight into the pathophysiology of sudden death and lead to improvements in therapy while establishing a framework for future fundamental discoveries.

我们提出了一个专业的在突然死亡的研究中心 犹他大学调查异常之间的关系 复极化和心律不齐的死亡。 调查组是 由17名调查员和8位顾问组成。 四个研究项目 并提出了四个核心支持实验室。 第一个研究 项目，人心室中复极的元素机制 由MC Sanguinetti执导的心肌细胞以K+和其他为中心 重复电流。 电压夹技术应用于分离 人体肌细胞确定存在哪些电流，以充分 表征它们并确定它们如何影响正常和异常 复极过程。 在项目2中，分子和生理学 长QT综合征，基地山和同事的机制调查 分子遗传学和心脏电生理学 人类和人体心肌中心律不齐的遗传形式。 JW梅森 和同事计划进行复极化变化的研究 紧接在第三个项目中缺血引起的突然死亡之前， 复极化变化与心室心律不齐和突然相关 死亡。 在项目4中，心心心态测量 复极化，无创测量的技术 心脏复极化和复极的差异是由 LS Green，RL Lux和人类诊断和预后的同事。 由梅森（Mason）领导的行政核心将为所有人提供帮助 预算管理和间交流的研究者。 核心2， 由WH Barry和Ga Peeter指导的细胞处理实验室将 为项目1和2准备孤立的人心肌细胞， 维护和培养项目1的青蛙卵母细胞，然后转化和 维持LQT患者DNA患者的外周血淋巴细胞 项目2中的分析核心3，复极信号处理 由Lux领导的实验室将提供心电图信号 项目2、3和4的处理和解释功能。 月亮指导第四核实验室，临床数据管理和 生物统计学，将协助项目2、3和4的研究人员 收集，统计分析和临床数据的解释 从患者获得。 该SCOR提案将重点放在问题的单个方面 突然死亡。 它结合基础科学与全器生理学 和复杂的临床医学。 它可能会洞悉 猝死的病理生理学并导致治疗的改善 建立一个未来基本发现的框架。