SCOR IN SUDDEN CARDIAC DEATH

心源性猝死中的 SCOR

• 批准号: 2029131
• 负责人: Jay W. Mason
• 金额: $ 131.38万
• 依托单位: UNIVERSITY OF UTAH
• 依托单位国家: 美国
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-02-01 至 1999-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2029131
• 关键词: arrhythmia; electrophysiology; sudden cardiac death

We propose a Specialized Center of Research in Sudden Death at the University of Utah to investigate the relationship between abnormalities of repolarization and arrhythmic death. The investigative group is composed of 17 investigators and 8 consultants. Four Research Projects and four Core support laboratories are proposed. The first research project, lonic Mechanisms of Repolarization in Human Ventricular Myocytes, directed by MC Sanguinetti, centers on K+ and other repolarizing currents. Voltage clamp techniques are applied to isolated human myocytes to determine which currents are present, to fully characterize them and to determine how they influence normal and abnormal repolarization processes. In Project 2, Molecular and Physiologic Mechanisms of the Long QT Syndrome, MT Keating and colleagues investigate the molecular genetics and the cardiac electrophysiology of this inherited form of arrhythmia in man and in human myocardium. JW Mason and coworkers plan studies of repolarization changes that occur immediately preceding sudden death due to ischemia in the third project, Repolarization Changes Associated with Ventricular Arrhythmias and Sudden Death. In Project 4, Electrocardiographic Measures of Ventricular Repolarization, techniques for non-invasive measurement of regional cardiac repolarization and disparity of repolarization are developed by LS Green, RL Lux and colleagues for diagnosis and prognostication in man. The Administrative Core, led by Mason, will provide assistance to all investigators in budget management and intercommunication. Core 2, the Cell Processing Laboratory directed by WH Barry and GA Peeters, will prepare isolated human ventricular myocytes for Projects 1 and 2, maintain and culture frog oocytes for Project 1, and transform and maintain peripheral blood lymphocytes from patients with LQT for DNA analysis in Project 2. Core 3, the Repolarization Signal Processing Laboratory led by Lux, will provide electrocardiographic signal processing and interpretation capabilities to Projects 2, 3 and 4. TE Moon directs the fourth Core laboratory, Clinical Data Management and Biostatistics, which will assist Project 2, 3 and 4 investigators in collection, statistical analysis and interpretation of clinical data obtained from patients. This SCOR proposal is sharply focussed on a single facet of the problem of sudden death. It combines basic science with whole-organ physiology and complex clinical medicine. It is likely to produce insight into the pathophysiology of sudden death and lead to improvements in therapy while establishing a framework for future fundamental discoveries.

我们建议在该中心设立一个专门的猝死研究中心 犹他大学调查异常之间的关系 复极和心律失常死亡。 调查组是 由 17 名调查员和 8 名顾问组成。 四个研究项目 并拟定四个核心支持实验室。 第一项研究 项目，人类心室复极的离子机制 MC Sanguinetti 指导的肌细胞以 K+ 和其他 复极化电流。 电压钳技术应用于隔离 人类肌细胞来确定存在哪些电流，以充分 描述它们的特征并确定它们如何影响正常和异常 再极化过程。 在项目 2 中，分子和生理学 MT Keating 及其同事研究了长 QT 综合征的机制 分子遗传学和心脏电生理学 人类和人类心肌的遗传性心律失常。 JW梅森 和同事计划研究发生的复极变化 在第三个项目中因缺血而猝死之前， 与室性心律失常和突发性心律失常相关的复极变化 死亡。 项目4，心室心电图测量 复极，区域非侵入性测量技术 心脏复极和复极差异是由 LS Green、RL Lux 及其同事对人类进行诊断和预测。 由梅森领导的行政核心将为所有人提供帮助 预算管理和相互沟通方面的调查员。 核心2， 由 WH Barry 和 GA Peeters 领导的细胞处理实验室将 为项目 1 和 2 准备分离的人心室肌细胞， 项目1的青蛙卵母细胞的维护和培养，以及转化和 维持 LQT 患者外周血淋巴细胞的 DNA 项目2.核心3，复极化信号处理中的分析 Lux领导的实验室将提供心电图信号 项目 2、3 和 4 的处理和解释能力。 TE Moon 领导第四个核心实验室：临床数据管理和 生物统计学，将协助项目 2、3 和 4 的研究人员 临床数据的收集、统计分析和解释 从患者身上获得。 该 SCOR 提案主要集中于问题的一个方面 猝死。 它将基础科学与全器官生理学相结合 和复杂的临床医学。 它可能会产生对 猝死的病理生理学并导致治疗的改进 为未来的基本发现建立框架。