DYNAMIC PROCESSES IN MYOGLOBIN

肌红蛋白的动态过程

基本信息

批准号：
6205982
负责人：
ETTORE BISMUTO
金额：
$ 3.19万
依托单位：
UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN
依托单位国家：
美国
项目类别：
财政年份：
1999
资助国家：
美国
起止时间：
1999-08-01 至 2000-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/6205982
关键词：
animal tissue biological products biomedical resource drug /agent

项目摘要

Tumor-directed drug delivery systems are being tested in mice. The use of fluorescent anti-tumor drugs has permitted evaluation of the effectiveness drug tissue targeting through the use of scanning techniques. Intensity scanning of tissue slices indicated targeting the drugs to tumor tissue but drugs were also found throughout the mouse tissue and in mouse organs. Activation of the drug induces a fluorescence lifetime change which suggested lifetime scanning as a potential method to discriminate between localization of inactive and active drug. In conjunction with ISS (Champaign, IL) a prototype scanning device was made to work with a standard ISS phase and modulation fluorometer. Preserved rat tissue slices were then scanned for intensity and fluorescence lifetime information. Phase contrast clearly indicated differences in tissues and provided preliminary evidence that lifetime imaging would be useful in discriminating drug activation from drug accumulation.

肿瘤定向药物输送系统正在小鼠身上进行测试。荧光抗肿瘤药物的使用已允许评估通过使用扫描来确定药物组织靶向的有效性技术。组织切片的强度扫描表明靶向这些药物作用于肿瘤组织，但在整个过程中也发现了药物小鼠组织和小鼠器官中。药物的激活会引起荧光寿命变化表明寿命扫描作为区分不活动和不活动定位的潜在方法活性药物。与国际空间站（伊利诺伊州尚佩恩）联合制作原型扫描装置可与标准国际空间站相位配合使用调制荧光计。然后扫描保存的大鼠组织切片获取强度和荧光寿命信息。相差清楚地表明了组织的差异并提供了初步的有证据表明终生成像可用于区分药物药物积累的激活。