3-DIMENSIONAL STRUCTURE AND FUNCTION OF SYNAPSES

突触的三维结构和功能

• 批准号: 6186173
• 负责人: KRISTEN M HARRIS
• 金额: $ 17.45万
• 依托单位: BOSTON UNIVERSITY (CHARLES RIVER CAMPUS)
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-09-30 至 2002-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6186173
• 关键词: Internet; bioimaging /biomedical imaging; calcium channel; calcium flux; computational neuroscience; computer human interaction; computer program /software; dendrites; developmental neurobiology; electron microscopy; hippocampus; information systems; interactive multimedia; laboratory rat; long term potentiation; mathematical model; neural plasticity; neuroanatomy; neurophysiology; phosphatidylinositols; psychic activity level; second messengers; spine; squirrel; synapses; technology /technique development

DESCRIPTION (Taken from application abstract): This project is directed at understanding the structure and function of synapses in the developing and mature brain, by quantitative and three-dimensional analysis of the structure, connectivity, and plasticity of presynaptic, postsynaptic, and glial elements. Such knowledge subserves understanding of the role of synaptic abnormalities in mental retardation. The informatics objective is to improve the methods of quantitative analysis and availability of anatomical data at the ultrastructural level by: 1. Developing an internet-accessible repository of synaptic and perisynaptic anatomy that will serve as a community resource for connecting the molecular and neuron levels of analyses. 2. Developing software for the public-domain that is internet accessible and third-party extensible to support and facilitate input and output of the database. 3. Improving the speed and accuracy of acquisition, alignment, calibration, segmentation, and 3D reconstruction of serial electron microscope sections from brain. 4. Developing software tools for quantitative comparison of different pre- and postsynaptic geometries and patterns of connectivity. The brain research objective is to test how synaptic structures can regulate synaptic function during development and plasticity by: 1. Investigating how synaptic and perisynaptic anatomy, change during development, during maintenance of slices in vitro, and during long-term potentiation (LTP) and long-term depression (LTD). 2. Examining how synaptic and perisynaptic anatomy differs during different levels of brain activity (hibernation, arousal, and wakeful activity). 3. Investigating the quantitative changes in spine and dendritic calcium due to activation of receptors coupled to the phosphoinositide second messenger system and determining the role this calcium signal plays in different types of synaptic plasticity (LTP, LTD, hibernation) using anatomical models from the database.

描述（取自应用程序摘要）：此项目针对 了解突触在发展中的突触的结构和功能 成熟的大脑，通过定量和三维分析 突触前，突触后和可塑性的结构，连通性和可塑性 神经元素。 这样的知识可以理解对 智力低下的突触异常。 信息学目标是 改善定量分析和可用性的方法 超微结构层面的解剖数据：1。 互联网可访问的突触和周突性解剖学存储库 将作为连接分子和神经元连接的社区资源 分析水平。 2。为公共域开发软件 互联网可访问和第三方可扩展以支持和促进 数据库的输入和输出。 3。提高速度和准确性 采集，对齐，校准，细分和3D重建 来自大脑的串行电子显微镜切片。 4。开发软件 用于定量比较不同前后的工具 几何和连通性模式。 大脑研究目标是测试突触结构如何调节 开发和可塑性期间的突触功能：1。 突触性和突触性解剖结构，发育过程中的变化如何 在体外和长期增强期间维护切片（LTP）和 长期抑郁症（Ltd）。 2。检查突触和闭环 解剖学在不同水平的大脑活动水平（休眠， 唤醒和清醒活动）。 3。调查定量变化 在脊柱和树突状的钙中，由于受体的激活与 磷酸肌醇第二信使系统并确定这一作用 钙信号以不同类型的突触可塑性（LTP，LTD， Hibernation）使用数据库中的解剖模型。