MICROVASCULAR MONITORING USING OPTICAL TECHNOLOGY

使用光学技术进行微血管监测

• 批准号: 2740010
• 负责人: JOHN STUART NELSON
• 金额: $ 25.93万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-02-01 至 2004-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2740010
• 关键词: bioimaging /biomedical imaging; biomedical equipment development; blood flow measurement; chick embryo; clinical research; dogs; heterodyning; human subject; image processing; microcirculation; nephelometry; noninvasive diagnosis; optics; phantom model; rapid diagnosis; tomography

DESCRIPTION (Adapted from Applicant's Abstract): The broad, long term objective of this project is the development of a noninvasive optical technique for imaging in vivo blood flow dynamics and tissue structure with high spatial resolution. Optical Doppler Tomography (ODT) combines Doppler velocimetry with optical coherence tomography (OCI) to measure blood flow velocity at discrete user-specified locations in highly scattering biological tissues. The exceptionally high resolution of ODT allows noninvasive imaging of both in vivo blood microcirculation and tissue structures surrounding the vessel. The rationale for using ODT to characterize the underlying microvasculature is that the technique is able to probe with high spatial resolution at discrete user-specified locations in biological tissues. Such localization is possible because the detected ODT interference fringe intensity gives accurate discrimination of the optical path length of Doppler shifted and backscattered light to within the source coherence length. Furthermore, in contrast to laser Doppler flowmetry, the overall ODT signal due to moving red blood cells (RBC) is almost entirely due to the Doppler shifted light. As a result, signal-to-noise ratios (SNR) are substantially higher. Inasmuch as tomographic images of blood flow and tissue structure can be obtained simultaneously from a single scan, ODT has decided advantages over existing methodologies. In preliminary studies conducted on in vitro and in vivo turbid samples and model vasculatures, respectively, the feasibility and potential application of ODT to characterize and image blood flow with high spatial resolution at discrete user-specified locations in highly scattering biological tissues was reported by the applicants to have been demonstrated. Further study appears warranted. The objective of the studies proposed herein is to extend these initial findings and was projected by the applicants to lead to the development of a new technology for the clinical management of patients in whom microvascular monitoring is essential.

描述（改编自申请人的摘要）：广泛的长期目标 该项目的重点是开发一种非侵入性光学技术 高空间成像体内血流动力学和组织结构 解决。光学多普勒断层扫描 (ODT) 将多普勒测速与 光学相干断层扫描 (OCI) 用于测量离散血流速度 高度分散的生物组织中用户指定的位置。这 ODT 的高分辨率可实现体内和体内的无创成像 血液微循环和血管周围的组织结构。这 使用 ODT 来表征潜在微脉管系统的基本原理是 该技术能够以离散的高空间分辨率进行探测 用户在生物组织中指定的位置。这样的本地化是可能的 因为检测到的 ODT 干涉条纹强度给出了准确的 多普勒频移和后向散射光路长度的判别 光在光源相干长度内。此外，与激光相比 多普勒血流测量，由于红细胞 (RBC) 移动而产生的总体 ODT 信号 几乎完全是由于多普勒频移光造成的。因此， 信噪比 (SNR) 显着提高。由于断层扫描 可以同时获得血流和组织结构的图像 单次扫描，ODT 与现有方法相比具有决定性优势。在 对体外和体内混浊样品和模型进行的初步研究 分别介绍了 ODT 的可行性和潜在应用 以高空间分辨率离散地表征血流并对其进行成像 报告了高度分散的生物组织中用户指定的位置 申请人已证明。似乎有必要进行进一步的研究。 本文提出的研究的目的是扩展这些最初的 申请人预计将导致开发 微血管病患者临床管理新技术 监控至关重要。