MICROBIAL GENOME PROJECT STREPTOCOCCUS MUTANS

微生物基因组计划变异链球菌

• 批准号: 6176048
• 负责人: Joseph J Ferretti
• 金额: $ 37.22万
• 依托单位: UNIVERSITY OF OKLAHOMA HLTH SCIENCES CTR
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-05-01 至 2002-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6176048
• 关键词: Streptococcus mutans; fungal genetics; genetic mapping; genome; nucleic acid sequence; Streptococcus mutans; fungal genetics; genetic mapping; genome; nucleic acid sequence

DESCRIPTION (Adapted from the Investigator's Abstract): The investigators propose to determine the complete genome sequence of Streptococcus mutans in this application. Decades of epidemiological, biochemical and animal studies have demonstrated that S. mutans is the principal causative agent of human dental caries (tooth decay). Dental caries is one of the most common infectious diseases afflicting humans, and in the United States, 84 percent of persons aged 17 years have evidence of present or past tooth decay. The cost of treating dental infections in the US is billions of dollars annually, and S. mutans is responsible for about 90 percent of these infections. Indeed, treatment of tooth decay caused by S. mutans accounts for about 50 percent of all US dental costs. Globally, the World Health Organization considers dental caries to be the most widespread oral disease in the world and reports that these infections are often untreated in many areas due to lack of available dental care. Additionally, S. mutans is occasionally associated with nonoral infections, principally subacute bacterial endocarditis. Considerable research effort and National Institute of Dental Research financial support has been expended in an effort to develop a vaccine to protect against S. mutans caused tooth decay, however, no truly effective strategy that prevents caries in humans has emerged. The principal investigator's laboratory was one of the first to investigate this organism at the molecular level and has been responsible for cloning, sequencing, and characterizing a number of genes including those encoding cell wall associated proteins, extracellular proteins involved with aggregation and production of adherent extracellular dextrans, and operons involved with the metabolism of sugars. Currently, the principal investigator's laboratory, in collaboration with Bruce A. Roe of the University of Oklahoma, Norman, is completing the sequence of the genome of Streptococcus pyogenes and assisting the laboratory of David W. Dyer of his department in the sequencing of the genome of Neisseria gonorrhoeae; both projects are funded by the NIH. The determination of the sequence of S. mutans will potentially lead to discovery of new and heretofore unknown genes and factors involved in virulence and pathogenicity, to new applications in molecular epidemiology and clinical diagnostics, to the elucidation of physiological factors essential for growth, and to rational approaches to new avenues to drug intervention and vaccine development.

描述（改编自调查员的摘要）：调查人员 提议确定链球菌突变体的完整基因组序列 此应用程序。 数十年的流行病学，生化和动物 研究表明，链球菌是 人牙龋（蛀牙）。 龋齿是最常见的 传染病困扰着人类，在美国，有84％ 17岁的人有现在或过去的蛀牙的证据。 这 美国治疗牙科感染的成本是数十亿美元 每年，S。Mutans负责其中约90％ 感染。 实际上，由S. mutans造成的蛀牙的治疗 大约有50％的美国牙科成本。 全球卫生 组织认为龋齿是最普遍的口腔疾病 在世界上，报道说这些感染在许多人中常常没有治疗 由于缺乏可用的牙科护理而导致的区域。 另外，S。Mutans是 偶尔与非口腔感染有关，主要是亚急性 细菌性心内膜炎。 大量的研究工作和国家研究所 为了努力 但是，开发一种疫苗以防止链球菌引起蛀牙，但是， 没有真正有效的策略来阻止人类龋齿的出现。 这 首席调查员的实验室是最早的调查的实验室之一 分子水平的有机体，一直造成克隆， 测序和表征许多基因，包括编码的基因 细胞壁相关蛋白，细胞外蛋白与 粘附的细胞外右旋和操纵子的聚集和生产 涉及糖的代谢。 目前，校长 调查员的实验室与Bruce A. Roe合作 俄克拉荷马大学，诺曼，正在完成基因组的顺序 链球菌为链球菌，并协助他的David W. Dyer实验室 在淋病奈瑟氏菌基因组的测序中；两个都 项目由NIH资助。 确定S的序列。 Mutans可能会导致发现新的和迄今未知的 毒力和致病性涉及的基因和因素， 分子流行病学和临床诊断中的应用 阐明生长至关重要的生理因素和理性 采用新途径的药物干预和疫苗开发方法。