IVEM TOMOGRAPHY OF CUBIC MEMBRANES IN CHAOS CAROLINSIS MITOCHONDRIA

混沌卡罗林线粒体立方膜的 IVEM 断层扫描

基本信息

批准号：
6119678
负责人：
YURU DENG
金额：
$ 0.57万
依托单位：
WADSWORTH CENTER
依托单位国家：
美国
项目类别：
财政年份：
1999
资助国家：
美国
起止时间：
1999-01-01 至 1999-12-31
项目状态：
已结题

项目摘要

(Supported by NSF DMB 8916315 to C.A. Mannella) Cubic membranes are symmetric, periodic structures that occur in a wide variety of living systems. There are several varieties of crystal structure proposed for the observed membrane arrays in different biological systems. In previous work (Deng, et al. Protozoology, in press), a template matching method was developed to indirectly identify the crystal type, using electron microscopy of amoeba mitochondria. In this work, we determined the crystal type from IVEM tomographic reconstructions, in comparison to a computer-generated model volume of a perfect crystal of the appropriate type. This validated the template-matching method. We made reconstructions of mitochondria representing examples of two different crystal types, the double-diamond and the gyroid. Extensive use of computer visualization and modeling methods enabled us to not only determine the crystal type but also to understand how the cubic membranes are integrated into the architecture of the mitochondrion, giving insights into their function. In the two examples, the spaces in the cubic area were shown to be intracristal space, which was connected to the space between the inner and outer mitochondrial membranes by tubular connecting regions, as seen in the other types of mitochondria we have studied (see CS subproject "Electron tomography of mitochondria: membrane interactions"). The matrix material was narrowly confined between the membranes in the cubic phase, thus reversing the usual situation in which most of the volume inside the mitochondria is matrix material. Dr. Deng completed her Ph.D. in Buffalo, then came to Albany as a postdoc in Dr. Mannella's lab, where she continued her structural studies. Her next priority will be functional studies on the cubic membranes. The work was formally presented at two meetings and also shown in connection with general talks on mitochondrial structure by Dr. Mannella, at several venues. A manuscript is ready for submission. Deng, Y., Mieczkowski, M., Marko, M., Buttle, K., Rath, B.K., Mannella, C.A. (1998) 3-D structure of periodic cubic-phase inner membranes in mitochondria of the amoebae Chaos carolinensis. Microscopy and Microanalysis vol 4, suppl. 2 (Proc. Microscopy and Microanalysis '98) Ed. G. Bailey, et al., Springer , New York, pp. 432-33. Deng, Y., Marko, M., Buttle, K., Leith, A., Mannella, C.A. (1998) Mitochondria in Chaos. Albany Conference on Frontiers of Mitochondrial Research. Deng, Y., Marko, M., Buttle, K., Mannella, C.A. (1998) Cubic mitochondrial membranes of the amoebae Chaos carolinsis studied by electron tomographic reconstruction. J. Structural Biol. (in preparation).

（由NSF DMB 8916315到C.A. Mannella支持）立方膜是对称的周期性结构，发生在多种多样的生活系统。有几种晶体结构建议针对不同生物学的观察到的膜阵列系统。在先前的工作中（Deng等，Protozoology，印刷中）开发了模板匹配方法以间接识别晶体类型，使用反向线粒体的电子显微镜。在这项工作，我们确定了IVEM层析成像的晶体类型与计算机生成的模型量相比，重建适当类型的完美水晶。这验证了模板匹配方法。我们进行了线粒体的重建代表两种不同晶体类型的例子，双钻石和能力。广泛使用计算机可视化和建模方法使我们不仅可以确定晶体类型，但也了解立方膜的状态集成到线粒体的架构中，提供见解进入他们的功能。在这两个示例中，立方体中的空间显示区域是室内空间，该空间连接到内部和外部线粒体膜之间的空间连接区域，如其他类型的线粒体所见研究（请参阅CS子标记“线粒体的电子断层扫描：膜相互作用”）。矩阵材料被狭窄地限制在立方阶段的膜之间，从而逆转了通常的线粒体内部大部分体积的情况是矩阵材料。邓博士完成了她的博士学位在布法罗，然后来了在Mannella博士实验室的后DOC到奥尔巴尼，在那里她继续她结构研究。她的下一个优先级将是关于立方膜。这项工作是在两次会议上正式提出的并且还与线粒体的一般谈判有关 Mannella博士在几个场所的结构。手稿已经准备好提交。 Deng，Y.，Mieczkowski，M.，Marko，M.，Buttle，K.， Rath，B.K.，Mannella，C.A。（1998）周期性的3-D结构在Amoebae混乱的线粒体中的立方相内膜 carolinensis。显微镜和微分析第4卷，补充。 2（Proc。显微镜和微分析'98）Ed。 G. Bailey等人，Springer，纽约，第432-33页。 Deng，M. Marko，M.，Buttle，K.，Leith，A.， Mannella，C.A。（1998）混乱中的线粒体。奥尔巴尼会议线粒体研究的前沿。 Deng，M. Marko，M.，Buttle，K。， Mannella，C.A。（1998）四边形的立方线粒体膜通过电子断层造影重建研究的混乱Carolinsis。 J. 结构生物。（准备）。