Digital design and fabrication of advanced biopurification materials

先进生物净化材料的数字化设计和制造

• 批准号: MR/W004399/1
• 负责人: Daniel Bracewell
• 金额: $ 32.67万
• 依托单位: University College London
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2021
• 资助国家: 英国
• 起止时间: 2021 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=MR%2FW004399%2F1
• 关键词: Digital; design; fabrication; advanced; biopurification

The global need for complex biological medicines such as cancer treatments and vaccines continues to grow. However the capability to manufacture high quality products at scale remains challenging with existing technology. Improvements to production capabilities and demand for products based on viral vectors and liposomes have resulted in exacerbated purification issues downstream, leading to performance and lifespan compromises of biomanufacturing unit operations such as filtration and chromatography. Solutions are required in order purify ever increasingly concentrated process streams in an efficient and cost-effective manner to meet growing demand for bio-therapeutics.The research proposed here utilises cutting-edge 3D imaging, analysis and digital design approaches in order to produce novel bioseparation materials that have clear advantages over existing purification technology. Applying methodology established at UCL structures can be designed, fabricated through 3D printing, analysed and improved through a design cycle. Pall Biotech, a leading international bioprocess vendor and applications expert, will provide secondment opportunities that will enable access to industrial experts and industrially relevant materials for this research in order to perform commercially applicable case studies.

全球对癌症治疗和疫苗等复杂生物药物的需求不断增长。但是，在现有技术方面，规模生产高质量产品的能力仍然具有挑战性。基于病毒媒介和脂质体的产品的生产能力和对产品需求的改善导致下游的纯化问题加剧，从而导致性能和寿命妥协，例如过滤和色谱等生物制造单元操作。必须以有效且具有成本效益的方式纯化纯净的过程流，以满足对生物治疗药的不断增长的需求。此处提出的研究利用尖端的3D成像，分析和数字设计方法来生产具有清除现有净化技术优势的新型生物份额材料。可以通过3D打印来设计，在UCL结构上建立的方法，并在设计周期中进行分析和改进。 PALL Biotech是一位领先的国际生物处理供应商和应用专家，将提供借调机会，使本研究的工业专家和与工业相关的材料访问，以便进行商业适用的案例研究。

项目成果

High-resolution imaging of depth filter structures using X-ray computed tomography

• DOI: 10.1007/s10853-021-06238-w
• 发表时间: 2021-06-18
• 期刊: JOURNAL OF MATERIALS SCIENCE
• 影响因子: 4.5
• 作者: Johnson, T. F.;Iacoviello, F.;Bracewell, D. G.
• 通讯作者: Bracewell, D. G.

Analysis of fouling and breakthrough of process related impurities during depth filtration using confocal microscopy.

• DOI: 10.1002/btpr.3233
• 发表时间: 2022-03
• 期刊: BIOTECHNOLOGY PROGRESS
• 影响因子: 2.9
• 作者: Parau, Maria;Johnson, Thomas F.;Pullen, James;Bracewell, Daniel G.
• 通讯作者: Bracewell, Daniel G.

Evaluating 3D-printed bioseparation structures using multi-length scale tomography.

• DOI: 10.1007/s00216-023-04866-6
• 发表时间: 2023-10
• 期刊: ANALYTICAL AND BIOANALYTICAL CHEMISTRY
• 影响因子: 4.3
• 作者: Johnson, Thomas F.;Conti, Mariachiara;Iacoviello, Francesco;Shearing, Paul R.;Pullen, James;Dimartino, Simone;Bracewell, Daniel G.
• 通讯作者: Bracewell, Daniel G.

Liposome Sterile Filtration Characterization via X-ray Computed Tomography and Confocal Microscopy.

• DOI: 10.3390/membranes11110905
• 发表时间: 2021-11-22
• 期刊: Membranes
• 影响因子: 4.2
• 作者: Johnson TF;Jones K;Iacoviello F;Turner S;Jackson NB;Zourna K;Welsh JH;Shearing PR;Hoare M;Bracewell DG
• 通讯作者: Bracewell DG

Evaluating 3D printed bioseparation structures using multi-length scale tomography

使用多长度尺度断层扫描评估 3D 打印的生物分离结构

• DOI: 10.22541/au.167587574.46873452/v1
• 发表时间: 2023
• 作者: Johnson T