KINETICS OF MICROBIAL DEGRADATION OF ORGANIC POLLUTANT MATERIALS

有机污染物的微生物降解动力学

• 批准号: 3777386
• 负责人: KENNETH F REARDON
• 金额: --
• 依托单位: COLORADO STATE UNIVERSITY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3777386
• 关键词: Pseudomonas; benzene; bioreactors; biotransformation; chloroform; environmental toxicology; fermentation; gas chromatography mass spectrometry; gram negative bacteria; microorganism culture; microorganism growth; microorganism metabolism; phenols; pollutant interaction; toxin metabolism; trichloroethylene; waste disposal; water pollution; water pollution control

The overall goals of this project are to investigate the kinetics of microbial degradation of pollutant mixtures and to apply this information toward the development of a novel bioreactor capable of removing a mixture of pollutants from water. Specifically, multiple substrate kinetics will be studies : benzene, phenol, and trichloroethylene consumption by Pseudomonas putida F1(and the inhibitory effects of chloroform) trichloroethylene and chloroform consumption by N. europaea benzene, phenol, trichloroethylene, and chloroform consumption by a mixed methanogenic culture Kinetic model parameters will be obtained by evaluating data from batch cultivations (yielding information on inhibition and high growth rate kinetics) and continuous cultivations (providing information on degradation kinetics at low growth rates). The case of trichloroethylene and phenol cometabolism by P. putida F1 will be examined in greater detail: in addition to the batch and steady-state continuous experiments, unsteady- state studies on both free and immobilized cells will be performed, and all of these data will be combined to develop an immobilized cell bioreactor capable of removing trichloroethylene (and phenol) from water. Currently, very little quantitative information is available on the rates and patterns of microbial degradation of multiple substrates. Many different types of interactions between substrates are possible, including cometabolism, inhibition, or even neutrality. Knowledge of these interactions and their kinetics is vital, not only for the design of bioreactors that can be used to remediate contaminated water but also for the prediction of the fate of pollutants in the environment, which is vital for the assessment of ecological and human health risks.

该项目的总体目标是研究动力学 污染物混合物的微生物降解并应用该信息 致力于开发能够去除混合物的新型生物反应器 来自水中的污染物。 具体来说，多底物动力学将 进行研究： 恶臭假单胞菌消耗苯、苯酚和三氯乙烯 F1(及氯仿的抑制作用) 欧洲猪笼草消耗的三氯乙烯和氯仿 苯、苯酚、三氯乙烯和氯仿的混合消耗量 产甲烷培养 动力学模型参数将通过评估批次数据获得 栽培（产生有关抑制和高生长率的信息 动力学）和连续培养（提供降解信息 低增长率时的动力学）。 三氯乙烯和苯酚案例 将更详细地检查恶臭假单胞菌 F1 的共同代谢： 除了批量和稳态连续实验外，非稳态 将对游离细胞和固定细胞进行国家研究，并且所有 这些数据将被结合起来开发固定细胞生物反应器 能够去除水中的三氯乙烯（和苯酚）。 目前，有关费率的定量信息非常少 以及多种基质的微生物降解模式。 许多 基材之间可能发生不同类型的相互作用，包括 共代谢、抑制甚至中性。 这些知识 相互作用及其动力学至关重要，不仅对于设计 生物反应器可用于修复受污染的水，也可用于 预测环境中污染物的去向，这一点至关重要 用于评估生态和人类健康风险。