ELASTICITY OF KINESIN UNDER ROTARY AND LINEAR FORCES

旋转力和线性力下驱动蛋白的弹性

• 批准号: 6171791
• 负责人: WILLIAM R. SCHIEF
• 金额: $ 3.24万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-30 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6171791
• 关键词: animal tissue; biomechanics; elasticity; kinesin; mechanical stress; microtubules; nanotechnology; video microscopy

Understanding how any motor works requires specifying the motor's mechanical components and determining how these components move and interact during the force-generating process. The internal workings of motor proteins, which power muscle contraction, propel swimming sperm, and carry organelles along tracks in cells, are not understood. This proposal intends to reveal the mechanical elastic components of the motor protein kinesin. The specific aims are: to measure the elasticity of kinesin under (1) torsional, (2) longitudinal, and (3) extensional forces, to locate the corresponding molecular compliant elements, and to evaluate the impact of these external forces on kinesin force generation. Optical tweezers and calibrated force fibers will be used to measure force and displacement of single-molecules for the characterization of motor elastic properties. Recombinant protein expression techniques will be utilized to identify the elastic components. The information obtained in this proposal can be used to develop a model for operation of the kinesin machine.

了解任何电动机的工作方式都需要指定电动机的机械组件，并确定这些组件在实力生成过程中如何移动和相互作用。尚不清楚运动蛋白的内部起作用，这些蛋白质的肌肉收缩，推动游泳精子并沿细胞中的轨道携带细胞器。该建议旨在揭示运动蛋白驱动蛋白的机械弹性成分。具体目的是：测量（1）扭转，（2）纵向和（3）延伸力下的动力蛋白的弹性，以定位相应的分子符号元件，并评估这些外部力对动能力量产生的影响。光学镊子和校准力纤维将用于测量单分子的力和位移，以表征运动弹性性能。重组蛋白表达技术将用于识别弹性成分。在本提案中获得的信息可用于开发用于操作的模型。