ELASTICITY OF KINESIN UNDER ROTARY AND LINEAR FORCES

旋转力和线性力下驱动蛋白的弹性

• 批准号: 6171791
• 负责人: WILLIAM R. SCHIEF
• 金额: $ 3.24万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-30 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6171791
• 关键词: animal tissue; biomechanics; elasticity; kinesin; mechanical stress; microtubules; nanotechnology; video microscopy

Understanding how any motor works requires specifying the motor's mechanical components and determining how these components move and interact during the force-generating process. The internal workings of motor proteins, which power muscle contraction, propel swimming sperm, and carry organelles along tracks in cells, are not understood. This proposal intends to reveal the mechanical elastic components of the motor protein kinesin. The specific aims are: to measure the elasticity of kinesin under (1) torsional, (2) longitudinal, and (3) extensional forces, to locate the corresponding molecular compliant elements, and to evaluate the impact of these external forces on kinesin force generation. Optical tweezers and calibrated force fibers will be used to measure force and displacement of single-molecules for the characterization of motor elastic properties. Recombinant protein expression techniques will be utilized to identify the elastic components. The information obtained in this proposal can be used to develop a model for operation of the kinesin machine.

了解电机的工作原理需要指定电机的机械部件，并确定这些部件在力产生过程中如何移动和相互作用。运动蛋白为肌肉收缩提供动力，推动精子游动，并沿着细胞内的轨道携带细胞器，但其内部工作原理尚不清楚。该提案旨在揭示运动蛋白驱动蛋白的机械弹性成分。具体目标是：测量驱动蛋白在（1）扭转力、（2）纵向力和（3）拉伸力下的弹性，定位相应的分子顺应元件，并评估这些外力对驱动蛋白力产生的影响。光镊和校准力纤维将用于测量单分子的力和位移，以表征运动弹性特性。将利用重组蛋白表达技术来鉴定弹性成分。该提案中获得的信息可用于开发驱动蛋白机器的操作模型。