WAVELET ENHANCEMENT OF CHROMOSOME BANDING PATTERNS

染色体带型的小波增强

• 批准号: 6181715
• 负责人: QIANG WU
• 金额: $ 26.06万
• 依托单位: ADVANCED DIGITAL IMAGING RESEARCH, LLC
• 依托单位国家: 美国
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-09-20 至 2003-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6181715
• 关键词: artificial intelligence; bioengineering /biomedical engineering; bioimaging /biomedical imaging; chromosome aberrations; chromosomes; computer data analysis; computer program /software; computer simulation; cytogenetics; digital imaging; image enhancement; image processing; molecular dynamics

This project aims to develop and commercialize significantly improved software for digital enhancement of the detail of chromosome banding patterns in microscopic images. These investigators have developed an innovative technique for this application, based upon wavelet transforms and multiresolution image analysis. Used with modern computerized chromosome analysis the proposed technique promises significantly improved enhancement of chromosome banding patterns and more effective visual detection of subtle rearrangements. This will help clinicians and researchers detect previously invisible or sub-visible band pattern alterations in conventional and high resolution banding. It will significantly increase the ability of automated instruments to assist the evaluation of chromosome alterations in clinical samples and in normal and neoplastic mammalian cells. During Phase I we implemented and tested three wavelet transforms with desirable mathematical properties. We developed a prototype multiresolution image processing system for chromosome enhancement. We obtained extremely encouraging results, strongly suggesting that these techniques offer considerably improved enhancement capability over conventional methods. and clearly demonstrating the feasibility of this approach. In Phase II we will complete the implementation and refinement of the software. We will implement several wavelet design approaches and evaluate many wavelet transform basis function sets that potentially can bring out relevant detail in chromosome banding patterns. PROPOSED COMMERCIAL APPLICATIONS: As soon as the new enhancement techniques are developed and qualified for routine application, they will be incorporated into PSII's PowerGene products, both in new systems sold and as an upgrade to existing systems.

该项目旨在开发和商业化显着改进的软件，以增强微观图像中染色体带模式的细节。这些研究者基于小波变换和多分辨率图像分析开发了针对此应用的创新技术。与现代计算机化染色体分析一起使用，提出的技术有望显着改善染色体带模式的增强，并更有效地对微妙的重排的视觉检测。这将有助于临床医生和研究人员在常规和高分辨率频段中检测到以前看不见或可见的带模式改变。它将显着提高自动化仪器的能力，以帮助评估临床样品以及正常和肿瘤哺乳动物细胞中的染色体改变。在第一阶段，我们用理想的数学属性实施并测试了三个小波变换。我们开发了用于染色体增强的原型多分辨率图像处理系统。我们获得了极其令人鼓舞的结果，强烈表明这些技术比常规方法具有大大提高的增强能力。并清楚地证明了这种方法的可行性。在第二阶段，我们将完成软件的实现和完善。我们将实施几种小波设计方法，并评估许多小波变换基函数集，这些函数可能会带来染色体带模式中相关的细节。拟议的商业应用程序：一旦开发新的增强技术并有资格用于常规应用程序，它们将被纳入PSII的Powergene产品中，无论是在出售的新系统和升级到现有系统中。