DISTORTION PRODUCT OTOACOUSTIC EMISSIONS--COCHLEAR FUNCT

失真产物耳声发射--耳蜗功能

基本信息

批准号：
6073695
负责人：
GLEN K. MARTIN
金额：
$ 17.69万
依托单位：
UNIVERSITY OF MIAMI SCHOOL OF MEDICINE
依托单位国家：
美国
项目类别：
财政年份：
1999
资助国家：
美国
起止时间：
1999-09-27 至 2002-08-31
项目状态：
已结题

项目摘要

The planned studies are designed to develop a rapid means of phenotyping mice according to the functional status of their peripheral ears. The initial experiments in Years 1-1.5 focus on developing a protocol using distortion-product otoacoustic emissions (DPOAEs) that is suitable for screening large numbers of mice for cochlear abnormalities. Toward this goal, 2f1-f2 DPOAEs will be obtained at three levels in the form of DPgrams that will test at 10 points per octave over a frequency extent that ranges from a geometric mean of 5.6 to 48.5 kHz (f2=60 kHz). These measures will be recorded from both ears of mice representing 40 unique strains, with n=12 mice from each strain. In addition, such DPOAE-screening data will be obtained at two ages in the same mice, ie, at 2 and 5 mo of age. The database created by these data will be analyzed in order to determine the 5th percentile of DPOAE levels in normal CBA-type mice and the 95th percentile in mice, such as the C57, with impaired cochlear function. The percentile data will be used to devise a standardized chart upon which data for individual mice undergoing mutagenesis can be plotted in order to determine if cochlear function is normal or impaired. In association with these experiments, a commercial instrument will be developed with a leading manufacturer of auditory-research equipment, which can be made available in the marketplace so that the scientific community will have access to the ability to determine the status of cochlear function in unknown mice. The second stage of the project, to be conducted in another group of mice from Years 1.5 to 3 of the study, is aimed at establishing a similar database for these same 40 mouse strains based on their susceptibility to noise-induced hearing loss. To accomplish this goal, following the acquisition of baseline DP-grams, at 3 mo mice will be exposed to a standard octave band noise known to cause permanent decrements in DPOAE levels, and then re-tested 2 wks later in order to document the effects of noise overexposure on cochlear function. Percentiles from these exposed mouse groups will form the basis of another standardized chart for determining the susceptibility of individual mice to acoustic overstimulation. Together, both projects will establish the usefulness of DPOAE testing for the purpose of developing simple and rapid screening methods of determining the status of cochlear function in individual mice. The resulting data will be made widely available on the internet in the form of databases and the relevant training manuals.

计划的研究旨在根据其周围耳朵的功能状态开发出一种快速的表型小鼠的手段。 1 - 1.5年的最初实验专注于使用变形产量的耳声排放（DPOAE）制定方案，适用于筛查大量小鼠的人工耳蜗异常。为了实现这一目标，将以DPGRAM的形式以三个级别获得2F1-F2 DPOAE，该级别将以每个八度的10分在一个频率范围内测试，范围从几何平均值为5.6到48.5 kHz（F2 = 60 kHz）。这些措施将记录在代表40个独特菌株的小鼠的两只耳朵中，每个菌株的n = 12只小鼠。此外，将在同一小鼠的两个年龄段（即2和5 mo）中获得此类DPOAE筛查数据。这些数据创建的数据库将进行分析，以确定正常CBA型小鼠中DPOAE水平的第5个百分点，而小鼠（例如C57）中的第95个百分位数（例如C57）具有受损的耳蜗功能。百分位数据将用于设计标准化图表，在该图表上，可以在该图表上绘制正在进行诱变的单个小鼠的数据，以确定耳蜗功能是正常还是受损。与这些实验相关，将使用一家领先的听觉研究设备制造商开发商业仪器，该制造商可以在市场中提供，以便科学界有能力确定未知小鼠中耳蜗功能的状态。该项目的第二阶段将在研究的另一组小鼠中进行，旨在基于它们对噪声引起的听力损失的敏感性，为这些40个小鼠菌株建立相似的数据库。为了实现这一目标，在获得基线DP-Grams之后，将在3个MO小鼠处接触到已知导致DPOAE水平永久降低的标准八度带噪声，然后稍后进行2 wks进行重新测试，以记录噪声过度暴露对手achlear功能的影响。这些暴露的小鼠组的百分位数将构成另一个标准化图表的基础，以确定单个小鼠对声学过度刺激的敏感性。这两个项目一起将建立DPOAE测试的有用性，目的是开发简单而快速的筛选方法来确定单个小鼠中耳蜗功能的状态。所得数据将以数据库和相关培训手册的形式在Internet上广泛使用。