PATCH CLAMP STUDIES OF DORSAL ROOT GANGLION CELLS AFTER NERVE TRAUMA

神经创伤后背根神经节细胞的膜片钳研究

• 批准号: 5215033
• 负责人: ROBERT H LAMOTTE
• 金额: --
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/5215033
• 关键词: acidity /alkalinity; action potentials; animal age group; capsaicin; dorsal root; electrophysiology; evoked potentials; hydrogen channel; laboratory rat; membrane activity; membrane permeability; membrane structure; neurophysiology; neurotrophic factors; norepinephrine; pain; paraganglia; peripheral nervous system; sciatic nerve; sodium channel; spinal cord injury; tetrodotoxin; tissue /cell culture; voltage /patch clamp; voltage gated channel

Dorsal root ganglion (DRG) cells can be a source of abnormal neuronal activity transmitted to the spinal cord after nerve injury. We will use whole-cell patch-clamp recordings to study differences in the membrane properties of dissociated DRG cells from adult rats that are normal or that have had a nerve injury consisting of a tight or a loose ligation. We will study the effects of nerve ligation on (1) the distribution and proportion of the macroscopic tetrodotoxin (TTX) sensitive and/or TTX resistant sodium currents to test whether "ligated cells" have a decrease in TTX resistant Na-currents, and (2) the proton and capsaicin evoked currents, in relation to TTX sensitivity, to test whether there is a more pronounced proton activated inward current, and whether protons are less effective in reducing the peak amplitude of the TTX-sensitive current in, ligated cells and (3) the norepinephrine-gated current to determine whether the norepinephrine evoked membrane depolarization is more pronounced in ligated cells and to determine whether alpha 1 or 2 receptors mediate the norepinephrine response. The results obtained are expected to provide new information on the origins of abnormal electrophysiological activity, in traumatized DRG cells, that might contribute to pain, dysesthesias and, in general, long term changes in the functional properties of certain spinal cord neurons.

背根神经节（DRG）细胞可能是异常神经元的来源 神经损伤后，活动传递到脊髓。 我们将使用 全细胞膜片钳记录以研究膜的差异 成年大鼠分离的 DRG 细胞的特性正常或 因结扎过紧或过松而造成神经损伤。 我们将研究神经结扎对（1）分布和 宏观河豚毒素 (TTX) 敏感和/或 TTX 的比例 抵抗钠电流来测试“结扎细胞”是否减少 在 TTX 抗性钠电流中，以及 (2) 诱发的质子和辣椒素 电流，与 TTX 灵敏度相关，以测试是否存在更多 明显的质子激活内向电流，以及质子是否较少 有效降低 TTX 敏感电流的峰值幅度， 连接细胞和（3）去甲肾上腺素门控电流以确定是否 去甲肾上腺素引起的膜去极化在 连接细胞并确定 α1 或 2 受体是否介导 去甲肾上腺素反应。 获得的结果有望提供有关起源的新信息 受损伤的 DRG 细胞中异常的电生理活动 可能会导致疼痛、感觉迟钝以及一般情况下的长期变化 某些脊髓神经元的功能特性。