ENZYMOLOGICAL ASPECTS OF NEURAL FUNCTION

神经功能的酶学方面

• 批准号: 3922450
• 负责人: R WAYNE ALBERS
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3922450
• 关键词: adenosinetriphosphatase; antibody; bioenergetics; catalyst; chemical structure function; conformation; enzyme mechanism; enzyme structure; gel electrophoresis; histochemistry /cytochemistry; ion transport; isozymes; monoclonal antibody; neural information processing; neurochemistry; phosphorylation; potassium; sodium; synthetic peptide; adenosinetriphosphatase; antibody; bioenergetics; catalyst; chemical structure function; conformation; enzyme mechanism; enzyme structure; gel electrophoresis; histochemistry /cytochemistry; ion transport; isozymes; monoclonal antibody; neural information processing; neurochemistry; phosphorylation; potassium; sodium; synthetic peptide

This project is comprised of research into the structure and functioning of ion transport systems. There are currently 3 subprojects. 1) Research into the topology of the catalytic subunit of the Na,K-ATPase. 2) A study of the regulation and expression of the Na,K-ATPase isoforms. These two subjects utilize site-directed antibodies raised against synthetic peptides as structural probes. 3) A study of the transient kinetics of the sodium pump The conformational changes that accompany the phosphorylation and dephosphorylation of the catalytic site of are under investigation. This subproject utilizes a rapid-quenching device for the study of phosphorylation reactions in the millisecond time range.

该项目包括对结构和 离子传输系统的功能。 目前有3个 子标题。 1）研究催化的拓扑 Na，K-ATPase的亚基。 2）对监管的研究和 Na，K-ATPase同工型的表达。 这两个主题利用 针对合成肽的位置定向抗体作为 结构探针。 3）对瞬时动力学的研究 钠泵伴随的构象变化 催化位点的磷酸化和去磷酸化 正在调查。 该子项目利用了快速衡量 用于研究磷酸化反应的装置 毫秒时间范围。