OMEGA 500 UPGRADE WITH ARRAY PROCESSOR

OMEGA 500 升级配备阵列处理器

• 批准号: 3520600
• 负责人: THOMAS L JAMES
• 金额: $ 19.1万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-03-05 至 1991-03-04
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3520600
• 关键词: nuclear magnetic resonance spectroscopy; nucleic acid structure; protein structure

We propose the acquisition of an upgraded console for a 500 MHz NMR spectrometer. The research proposed for the new instrument will improve our capability for determining high-resolution protein and nucleic acid structures in solution from two-dimensional NMR experiments. The most effective means of deriving nucleic acid and protein solution structure by using the complete relaxation matrix analysis of 2D NOE spectra in conjunction with the computational techniques of distance geometry, molecular mechanics and restrained molecular dynamics will be explored. Reverse-detection heteronuclear 2D NMR experiments will enable resonance assignments and structural insights. Some studies necessitate the use of H2O solutions, thus requiring efforts to overcome the dynamic range problem due to water protons. The distance information obtained via 2D NOE spectra will be augmented by dihedral angle constraints derived from scaler coupling-based 2D NMR experiments. The improved capabilities should also aid in our studies of ligand binding to biopolymers, thus leading to greater mechanistic insights.

我们建议购买 500 MHz NMR 的升级控制台 光谱仪。 为新仪器提出的研究将得到改进 我们测定高分辨率蛋白质和核酸的能力 二维核磁共振实验中溶液中的结构。 最 衍生核酸和蛋白质溶液结构的有效方法 使用 2D NOE 谱的完整弛豫矩阵分析 结合距离几何的计算技术， 将探索分子力学和约束分子动力学。 反向检测异核二维核磁共振实验将实现共振 任务和结构见解。 一些研究需要使用 H2O 解决方案，因此需要努力克服动态范围问题 由于水质子。 通过二维NOE光谱获得的距离信息 将通过从缩放器导出的二面角约束来增强 基于耦合的二维核磁共振实验。 改进的能力还应该 帮助我们研究配体与生物聚合物的结合，从而导致 更深刻的机械见解。