DYnamic control in hybrid plasmonic NAnopores: road to next generation multiplexed single MOlecule detection

混合等离子体纳米孔的动态控制：下一代多重单分子检测之路

• 批准号: EP/X038009/1
• 负责人: Ulrich Keyser
• 金额: $ 33.8万
• 依托单位: University of Cambridge
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2023
• 资助国家: 英国
• 起止时间: 2023 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=EP%2FX038009%2F1
• 关键词: DYnamic; control; hybrid; plasmonic; NAnopores

In the quest to develop platforms to decipher the information in biomolecules there is an increasingly demand for techniques that are capable of identifying specific biomolecules at the smallest possible concentration, controlling their capturing and movement at the nanoscale. Nanopore sequencing is a key technology to drive this progress, and solid-state nanopores can be a major technological player. In the field of solid-state nanopores, many challenges remain in particular toward i) design and fabricate nanopore with size comparable to biological pores, ii) dynamic control of single molecule translocation, and iii) multiplexed read-out. Progress toward integrated platforms where both solid-state and biological nanostructures are implemented has been brought by the development of functional nanomaterials and DNA nanotechnology, able to address the importance of nanoscale precision in nanopore platforms with the potential to surpass the performance of biological nanopores. Yet, the integration of biological and synthetic nanomaterials is technically challenging, and their use to fully control and measure single molecule is far from real-world applications. DYNAMO is designed as an innovative research and training network, where we will recruit 10 Researchers to work toward the ambitious goal of developing the next-generation single molecule technologies exploiting hybrid DNA and metallic nanostructures. The network brings together a unique team of 8 world-leading academics and 1 high tech innovative company at the forefront of optical spectroscopy, DNA nanostructures, nanopore technology developments, single molecule detection and control, and biotech real-world applications. DYNAMO will establish an intersectoral training and research programme at the physics/chemistry/biotech interface with partners from 6 EU countries, aimed at creating the next generation of skilled well-connected scientists that will pioneer the 'single molecule science of tomorrow'.

为了开发平台以解读生物分子中的信息，对技术的需求越来越多，能够以最小的可能浓度识别特定的生物分子，从而控制其在纳米级的捕获和移动。纳米孔测序是推动这一进度的关键技术，固态纳米孔可能是主要的技术参与者。在固态纳米孔领域，尤其是针对i）设计和制造纳米孔，其大小与生物孔相当，ii）单分子易位的动态控制，iii）多路复用读出。在实施固态和生物纳米结构的集成平台方面，通过功能性纳米材料和DNA纳米技术的开发实现了固态和生物纳米结构，能够解决纳米级精度在纳米孔平台中的重要性，从而有可能超越生物纳米磁场的性能。然而，生物学和合成纳米材料的整合在技术上具有挑战性，并且它们用于完全控制和测量单分子远非现实应用。 Dynamo被设计为创新的研究和培训网络，我们将招募10位研究人员朝着开发利用混合DNA和金属纳米结构的下一代单分子技术的雄心勃勃的目标。该网络汇集了一支由8个世界领先的学者组成的独特团队，以及一家高科技创新公司，位于光谱，DNA纳米结构，纳米技术发展，单分子检测和控制以及生物技术现实世界中的最前沿。 Dynamo将与来自6个欧盟国家的合作伙伴在物理/化学/生物技术界面上建立部门培训和研究计划，旨在创建下一代熟练的熟练良好连接的科学家，这些科学家将开拓“明天的单个分子科学”。