Peptide adsorption on metal oxide surfaces. Investigating the biomaterial/biological interface with synchrotron radiation.

金属氧化物表面上的肽吸附。

• 批准号: EP/V002341/1
• 负责人: Andrew THOMAS
• 金额: $ 4.59万
• 依托单位: University of Manchester
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2021
• 资助国家: 英国
• 起止时间: 2021 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=EP%2FV002341%2F1
• 关键词: Peptide; adsorption; metal; oxide; surfaces.

The interaction between biological molecules and inorganic material surfaces is of importance in a number of areas from biomedical devices for implantation, sensors and bioreactors. The required behaviours for each of these applications are slightly different, for example in biomedical implants it is thought that a protein conditioning layer is formed at the surface, prior to cell attachment and growth of new tissue. On the other hand, in pharmaceutical applications where protein therapeutics may be manufactured, interactions with the reactor material surfaces can lead to denaturation or agglomeration of the proteins. This may have the effect of removing the desired action protein, or in the worse case, causing unwanted side effects such as protein aggregation.Studies of molecular adsorption on well-characterised surfaces using synchrotron techniques have led to an improved understanding of protein-metal interactions. However, in most cases the surface of a metal implant or pipeline will be terminated with a native oxide layer. In addition, these studies are often carried out under vacuum conditions, thus the effect of water on the adsorption process is not well understood. Obviously in a real biomedical device or pharmaceutical plant, water will be a major component of the surrounding media. Recent advances in photoelectron spectroscopy now allow measurements to be made in the presence of water and water vapour. Photoelectron spectroscopy is an extremely powerful probe of surface chemistry and the adsorption mechanism of molecules, since it is capable of studying just the top 1 - 10 nm of a material surface.In this proposal we will study the adsorption processes of five different amino acids which exhibit varying degrees of acidity/basicity and two small peptides on idealised titanium dioxide surfaces. Rutile titanium dioxide is the native oxide formed on titanium which is widely used both as a metal and alloy in biomedical implants, and is believed to be a key factor in the success of these devices.

生物分子与无机材料表面之间的相互作用在许多领域从生物医学设备进行的许多区域都很重要。这些应用中每一种的所需行为都略有不同，例如在生物医学植入物中，人们认为在细胞附着和新组织的生长之前，在表面形成蛋白质调节层。另一方面，在可以制造蛋白质疗法的药物应用中，与反应堆材料表面的相互作用可以导致蛋白质的变性或聚集。这可能具有去除所需的作用蛋白的作用，或者在更糟糕的情况下，会引起不必要的副作用，例如蛋白质聚集。使用同步基因技术研究了分子吸附对良好特征表面的研究，从而提高了人们对蛋白质 - 金属相互作用的理解。但是，在大多数情况下，金属植入物或管道的表面将用天然氧化物层终止。此外，这些研究通常是在真空条件下进行的，因此水对吸附过程的影响尚不清楚。显然，在真正的生物医学设备或制药厂中，水将是周围介质的主要组成部分。现在，光电子光谱法的最新进展允许在存在水和水蒸气的情况下进行测量。光电子光谱镜是表面化学和分子的吸附机制的极其强大的探针，因为它能够仅研究材料表面的前1-10 nm的顶部1-10 nm。在该建议中，我们将研究五种不同的氨基酸的吸附过程，这些氨基酸的吸附过程在酸性/碱性和两个小peptium the Inseliment titan curfium of Indortial titan surff the curfium and surfiim curfium curfium curfium curf。金红石二氧化钛是钛在生物医学植入物中被广泛用作金属和合金的天然氧化物，被认为是这些设备成功的关键因素。