Peptide adsorption on metal oxide surfaces. Investigating the biomaterial/biological interface with synchrotron radiation.

金属氧化物表面上的肽吸附。

• 批准号: EP/V002341/1
• 负责人: Andrew THOMAS
• 金额: $ 4.59万
• 依托单位: University of Manchester
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2021
• 资助国家: 英国
• 起止时间: 2021 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=EP%2FV002341%2F1
• 关键词: Peptide; adsorption; metal; oxide; surfaces.

The interaction between biological molecules and inorganic material surfaces is of importance in a number of areas from biomedical devices for implantation, sensors and bioreactors. The required behaviours for each of these applications are slightly different, for example in biomedical implants it is thought that a protein conditioning layer is formed at the surface, prior to cell attachment and growth of new tissue. On the other hand, in pharmaceutical applications where protein therapeutics may be manufactured, interactions with the reactor material surfaces can lead to denaturation or agglomeration of the proteins. This may have the effect of removing the desired action protein, or in the worse case, causing unwanted side effects such as protein aggregation.Studies of molecular adsorption on well-characterised surfaces using synchrotron techniques have led to an improved understanding of protein-metal interactions. However, in most cases the surface of a metal implant or pipeline will be terminated with a native oxide layer. In addition, these studies are often carried out under vacuum conditions, thus the effect of water on the adsorption process is not well understood. Obviously in a real biomedical device or pharmaceutical plant, water will be a major component of the surrounding media. Recent advances in photoelectron spectroscopy now allow measurements to be made in the presence of water and water vapour. Photoelectron spectroscopy is an extremely powerful probe of surface chemistry and the adsorption mechanism of molecules, since it is capable of studying just the top 1 - 10 nm of a material surface.In this proposal we will study the adsorption processes of five different amino acids which exhibit varying degrees of acidity/basicity and two small peptides on idealised titanium dioxide surfaces. Rutile titanium dioxide is the native oxide formed on titanium which is widely used both as a metal and alloy in biomedical implants, and is believed to be a key factor in the success of these devices.

生物分子和无机材料表面之间的相互作用在植入生物医学设备、传感器和生物反应器等许多领域中都非常重要。这些应用中的每一个所需的行为都略有不同，例如在生物医学植入物中，人们认为在细胞附着和新组织生长之前在表面形成蛋白质调理层。另一方面，在可以制造蛋白质治疗剂的制药应用中，与反应器材料表面的相互作用可能导致蛋白质变性或聚集。这可能会去除所需的作用蛋白，或者在更糟糕的情况下，导致不必要的副作用，例如蛋白质聚集。使用同步加速器技术对充分表征的表面上的分子吸附进行的研究提高了对蛋白质-金属相互作用的理解。然而，在大多数情况下，金属植入物或管道的表面将以自然氧化层终止。此外，这些研究通常在真空条件下进行，因此水对吸附过程的影响尚不清楚。显然，在真实的生物医学设备或制药厂中，水将是周围介质的主要组成部分。光电子能谱的最新进展现在允许在水和水蒸气存在的情况下进行测量。光电子能谱是表面化学和分子吸附机制的极其强大的探针，因为它能够仅研究材料表面的顶部 1 - 10 nm。在本提案中，我们将研究五种不同氨基酸的吸附过程，在理想化的二氧化钛表面上表现出不同程度的酸度/碱度和两个小肽。金红石二氧化钛是钛上形成的天然氧化物，广泛用作生物医学植入物中的金属和合金，并被认为是这些设备成功的关键因素。