High Resolution High Throughput Mass Spectrometry to Characterise Materials, Chemicals, and BioCatalysts

用于表征材料、化学品和生物催化剂的高分辨率高通量质谱

• 批准号: EP/T019328/1
• 负责人: Perdita Barran
• 金额: $ 140.09万
• 依托单位: University of Manchester
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2020
• 资助国家: 英国
• 起止时间: 2020 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=EP%2FT019328%2F1
• 关键词: Resolution; Throughput; Mass; Spectrometry; Characterise; Resolution; Throughput; Mass; Spectrometry; Characterise

This proposal will equip UoM new equipment that will enable high throughput high resolution ion mobility mass spectrometry analysis. This bid will increase capacity for analysis and extend our capacity to measure a wide range of analytes. Specialist equipment will be used to measure the mass and the shape of a large number of newly synthesised chemicals. In the simplest terms possible, mass spectrometry measures the movement of ions in a vacuum to give mass and ion mobility mass spectrometry measures the movement of ions in a gas to give shape. If we measure mass very accurately we can work out what atoms a molecule is made of and if we measure the shape very accurately we can work out how the atoms are arranged. So for a large range of different molecules we will be able to tell what we have made and how much we have made. The PI has been making ion mobility mass spectrometers and using ion mobility mass spectrometry for over 20 years. She has pioneered its use in many areas of chemistry and in this proposal we plan to do it even faster and with more accuracy. This equipment will be located with the Manchester Institute for Biotechnology in the Michael Barber Centre for Collaborative Mass Spectrometry. It will be used by research teams at the University of Manchester as well as by external users from both academia and instrument. We will use this state of the art instrumentation to underpin investments by EPRSC in the Future Biomanufacturing Research hub and the Prosperity Partnership with AstraZeneca as well as other programs in materials and catalysis. This new capability will consist of two mass spectrometers coupled to novel ionisation sources. A State-of-the-art Cyclic IMS QTof MS instrument will provide unique enhanced analytical capabilities for molecular identification and structural characterisation of complex mixtures complete with full ion mobility data. A second "work-horse" DESI interfaced Synapt XS instrument will provide broader research access and wider application, and act as an initial screening platform prior to Cyclic Structural work for rapid mass spectrometry analysis. The ionisation sources will enable analysis with minimal sample preparation both directly from surfaces via Desorption Electrospray ionisation and with microfluidic droplet dispensing to provide a unique ultra-high-throughput (uHTP) screening capability. This will provide mass and structural data for a range of chemical analytes from small molecules to polymers, supramolecular compounds to biopharmaceuticals.

该提案将为UOM新设备配备，该设备将使高吞吐量高分辨率离子迁移率质谱分析。该出价将增加分析能力，并扩展我们测量广泛分析物的能力。专业设备将用于测量大量新合成化学品的质量和形状。从最简单的角度来看，质谱法测量了真空中离子的移动，使质量和离子迁移率质谱法测量了气体中离子的运动以产生形状。如果我们非常准确地测量质量，我们可以弄清分子是由分子制成的原子，如果我们非常准确地测量形状，则可以算出原子的排列方式。因此，对于各种不同的分子，我们将能够说出我们所做的是什么以及所做的。 PI一直在制造离子迁移率质谱仪和使用离子迁移率质谱法，已有20多年的历史了。她率先在许多化学领域中使用了它的使用，在此提案中，我们计划更快，更准确地做到这一点。 该设备将位于Michael Barber合作质谱中心的曼彻斯特生物技术学院。曼彻斯特大学的研究团队以及学术界和仪器的外部用户都将使用它。我们将使用这种最先进的工具来支撑EPRSC的未来生物制造研究中心的投资，以及与阿斯利康的繁荣合作伙伴关系以及其他材料和催化方案。这种新功能将由两个质谱仪组成，这些质谱仪与新型电离源结合。最先进的循环IMS QTOF MS仪器将提供独特的增强分析能力，用于分子鉴定和结构表征，并具有完整的离子迁移率数据。第二个“ Work Horse” Desi接口XS仪器将提供更广泛的研究访问和更广泛的应用，并在循环结构工作之前充当初始筛选平台，用于快速质谱分析。电离源将通过解吸电喷雾电离直接从表面和微流体液滴分配来直接从表面进行分析，以提供独特的超高通量（UHTP）筛选能力。这将为从小分子到聚合物，超分子化合物再到生物药物的一系列化学分析物提供质量和结构数据。