LINKAGE ANALYSIS AND MULTIPLE LOCI

连锁分析和多位点

• 批准号: 3446518
• 负责人: DAVID T BISHOP
• 金额: $ 5.4万
• 依托单位: UNIVERSITY OF UTAH
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-01-01 至 1986-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3446518
• 关键词: biological polymorphism; family; genetic counseling; genetic mapping; genetic markers; human population genetics; linkage mapping

The research seeks to investigate the current methods of major gene localization through linkage studies and to develop new methods where appropriate. Particular attention will be paid to localizing disease loci. General methods for estimating the expected amount of information available in a particular pedigree structure will be developed, so that even for complex traits, it is possible to evaluate the utility of performing a linkage study. The classical methodology of LOD scores for two loci will be investigated to examine the power of the method, the estimates of recombination fraction obtained and the sample sizes required for accurate estimation of the recombination fraction. Various family structures will be examined to develop strategies for efficient mapping. These studies will be extended to linkage between three loci. In particular, the problem of determining gene order will be examined. Discussions of power for the three loci analysis will also consider the sample sizes required to infer interference or gene conversion. A simulation study will be undertaken to examine the genetic analysis of common disorders. In particular, the effect of not allowing the sporadic occurrence of the disorder on both pedigree and linkage analysis will be considered.

该研究旨在探讨主要基因的当前方法 通过连锁研究进行本地化并开发新方法 合适的。 将特别注意局部疾病 基因座。 估计预期信息量的一般方法 将开发特定谱系结构中可用的内容，以便 即使对于复杂的特征，也可以评估其效用 进行连锁研究。 将研究两个基因座的 LOD 评分的经典方法 为了检查该方法的功效，重组分数的估计值 获得的数据以及准确估计所需的样本量 重组分数。 将审查各种家庭结构，以 制定有效绘图的策略。 这些研究将被扩展 三个基因座之间的连锁。 特别是确定问题 将检查基因顺序。 三个位点功效的讨论 分析还将考虑推断干扰所需的样本量 或基因转换。 将进行模拟研究来检查遗传分析 常见疾病。 特别是不允许零星的影响 系谱和连锁分析中该疾病的发生将是 经过考虑的。