LINKAGE ANALYSIS AND MULTIPLE LOCI

连锁分析和多位点

• 批准号: 3446518
• 负责人: DAVID T BISHOP
• 金额: $ 5.4万
• 依托单位: UNIVERSITY OF UTAH
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-01-01 至 1986-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3446518
• 关键词: biological polymorphism; family; genetic counseling; genetic mapping; genetic markers; human population genetics; linkage mapping

The research seeks to investigate the current methods of major gene localization through linkage studies and to develop new methods where appropriate. Particular attention will be paid to localizing disease loci. General methods for estimating the expected amount of information available in a particular pedigree structure will be developed, so that even for complex traits, it is possible to evaluate the utility of performing a linkage study. The classical methodology of LOD scores for two loci will be investigated to examine the power of the method, the estimates of recombination fraction obtained and the sample sizes required for accurate estimation of the recombination fraction. Various family structures will be examined to develop strategies for efficient mapping. These studies will be extended to linkage between three loci. In particular, the problem of determining gene order will be examined. Discussions of power for the three loci analysis will also consider the sample sizes required to infer interference or gene conversion. A simulation study will be undertaken to examine the genetic analysis of common disorders. In particular, the effect of not allowing the sporadic occurrence of the disorder on both pedigree and linkage analysis will be considered.

该研究旨在研究主要基因的当前方法 通过链接研究进行本地化，并开发新方法 合适的。 特别关注本地疾病 基因座。 估计预期信息量的一般方法 将开发在特定的血统结构中，以便 即使对于复杂的特征，也可以评估 进行连锁研究。 将研究两个基因座的LOD分数的经典方法论 为了检查该方法的功能，重组分数的估计值 获得的以及需要准确估计的样本量 重组分数。 各种家庭结构将进行检查 制定有效映射的策略。 这些研究将扩展 与三个基因座之间的联系。 特别是确定的问题 将检查基因顺序。 关于三个基因座的权力的讨论 分析还将考虑推断干扰所需的样本量 或基因转换。 将进行一项模拟研究，以检查 常见疾病。 特别是，不允许零星的效果 该疾病在血统和连锁分析中的发生将是 经过考虑的。