PATHOBIOLOGICAL DETERMINANTS OF ATHEROSCLEROSIS IN YOUTH

青年动脉粥样硬化的病理学决定因素

• 批准号: 3433063
• 负责人: FREDERICK J CORNHILL
• 金额: $ 13.47万
• 依托单位: OHIO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1985
• 资助国家: 美国
• 起止时间: 1985-06-01 至 1992-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3433063
• 关键词: adolescence (12-20); atherosclerosis; biomedical facility; cardiovascular disorder epidemiology; cooperative study; disease /disorder proneness /risk; human subject; image processing; microscopy; morphology; pathology; postmortem; tissue /cell preparation; young adult human (21-34); adolescence (12-20); atherosclerosis; biomedical facility; cardiovascular disorder epidemiology; cooperative study; disease /disorder proneness /risk; human subject; image processing; microscopy; morphology; pathology; postmortem; tissue /cell preparation; young adult human (21-34)

This is a 4-phase, investigator initiated, multicenter study. Its major purpose is to study important unanswered questions concerning the pathogenesis of human atherosclerosis using modern pathobiological approaches. To accomplish this, autopsies on persons (15-35 yr) killed by trauma will be done under rigorously controlled protocols to supply specified tissue samples for standardized biochemical and morphologic studies. The morphometric and biochemical analysis will be performed at designated Central Laboratories; these are: histotechnique (Univ of Chicago), morphometry (Ohio State Univ), and lipid biochemistry (Louisiana State Univ, New Orleans). The study design and all of the statistical aspects of the common protocol sections of the study, including the analysis of a major portion of the data, will be accomplished in the Central Statistical and Data Analysis Laboratory in San Antonio. The Central Laboratory at LSU will organize the data on risk factors from each case and will do most of the analyses on the post-mortem samples of blood. In this application the role of the Ohio State Univ in specimen collection and the activity of the Morphometry Central Laboratory (MCL) are presented. The Ohio State Univ will contribute 125 sets of specimens per year to the multicenter study. The MCL will analyze over 100,000 microscopic and macroscopic images generated in the study. The MCL will produce tabular data (e.g., lesion thickness) and spatial data (e.g., probability-of-occurrence maps). Among the major questions being addressed using data from the morphometry center are: 1) Do some fatty streaks progress to fibrous plaques and, if so, can the morphological characteristics of a transitional lesion be defined? 2) What is the evolutionary patter of different lesion types with increasing age? 3) What are the morphometric changes associated with progression? 4) What are the incidence and morphologic features of insudative (gelatinous) lesions and does the probability-of-occurrence map for these correlate with that of subsequent proliferative disease? 5) What structural features of lesions can be correlated with specific risk factors? 6) Are there morphometric differences in lesion structure between sexes that can account for the well-known differences in clinical course? 7) Do atherosclerotic structural features or risk factors data for young people provide any insight into the decline of coronary heart disease in the United States?

这是一项四相研究者启动的多中心研究。 它的专业 目的是研究有关该问题的重要问题 使用现代病理学的人动脉粥样硬化的发病机理 方法。 为此，对被人（15 - 35年）的人尸检 创伤将在严格控制的协议下进行以供应 标准化生化和形态学的指定组织样品 研究。 形态计量学和生化分析将在 指定的中央实验室；这些是：Histotechnique（Univ 芝加哥），形态计量学（俄亥俄州立大学）和脂质生物化学（路易斯安那州 国家大学，新奥尔良）。 研究设计和所有统计 研究的共同协议部分的各个方面，包括 对数据的一部分分析将在 圣安东尼奥市的中央统计和数据分析实验室。 这 LSU的中央实验室将组织有关每个风险因素的数据 病例并将对血液验尸样本进行大多数分析。 在此应用中，俄亥俄州立大学在标本集合中的作用 形态计量学中央实验室（MCL）的活性是 提出。 俄亥俄州立大学将贡献125套标本 多中心研究的一年。 MCL将分析超过100,000 研究中产生的显微镜和宏观图像。 MCL会 产生表格数据（例如，病变厚度）和空间数据（例如， 出现图的概率）。 在解决的主要问题中 使用形态计量学中心的数据是：1）做一些脂肪条纹 进展到纤维斑块，如果是的，则可以形态学 定义过渡病变的特征？ 2）什么是 年龄增加的不同病变类型的进化模式？ 3）什么 形态计量计是否与进展有关？ 4）什么是 急诊（凝胶状）病变的发病率和形态特征和形态特征 这些概率的概率图映射是否与 随后的增生性疾病？ 5）病变的结构特征 可以与特定风险因素相关吗？ 6）是否存在形态计量学 性别之间的病变结构的差异可以解释 临床过程中众所周知的差异？ 7）进行动脉粥样硬化 年轻人的结构特征或风险因素数据提供任何 深入了解美国冠心病的衰落？